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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2113 [new locus tag: SAUSA300_RS11640 ]
  • pan locus tag?: SAUPAN005508000
  • symbol: SAUSA300_2113
  • pan gene symbol?: dacA
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2113 [new locus tag: SAUSA300_RS11640 ]
  • symbol: SAUSA300_2113
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2288286..2289095
  • length: 810
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTTTTCCAACTTTTTTCAAAACCTCAGTACGTTAAAAATTGTAACGAGTATCCTT
    GATTTACTGATAGTTTGGTATGTACTTTATCTTCTCATCACGGTCTTTAAGGGAACTAAA
    GCGATACAATTACTTAAAGGGATATTAGTAATTGTTATTGGTCAGCAGATAAGTATGATA
    TTGAACTTGACTGCAACATCTAAATTATTCGATATCGTTATTCAATGGGGGGTATTAGCT
    TTAATAGTAATATTCCAACCAGAAATTAGACGTGCGTTAGAACAACTTGGTAGAGGTAGC
    TTTTTAAAACGCTATACTTCTAATACGTATAGTAAAGATGAAGAGAAATTGATTCAATCG
    GTTTCAAAGGCTGTGCAATATATGGCTAAAAGACGTATAGGTGCATTAATTGTCTTTGAA
    AAAGAAACAGGTCTTCAAGATTATATTGAAACAGGTATTGCAATGGATTCAAATATTTCG
    CAAGAACTTTTAATTAATGTCTTTATACCTAACACACCTTTACATGATGGTGCAATGATT
    ATTCAAGGCACGAAGATTGCAGCAGCAGCAAGTTATTTGCCATTGTCTGATAGTCCTAAG
    ATATCTAAAAGTTTGGGTACAAGACATAGAGCTGCGGTTGGTATTTCAGAAGTATCTGAT
    GCATTTACCGTTATTGTATCTGAAGAAACTGGTGATATTTCGGTAACATTTGATGGAAAA
    TTACGACGAGACATTTCAAACGAAATTTTTGAAGAGTTGCTTGCTGAACATTGGTTTGGC
    ACACGCTTTCAAAAGAAAGGTGTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    810

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2113 [new locus tag: SAUSA300_RS11640 ]
  • symbol: SAUSA300_2113
  • description: hypothetical protein
  • length: 269
  • theoretical pI: 9.19244
  • theoretical MW: 30078.9
  • GRAVY: 0.250929

Function[edit | edit source]

  • reaction:
    EC 2.7.7.85?  ExPASy
    Diadenylate cyclase 2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00159 family protein (TIGR00159; HMM-score: 295.4)
  • TheSEED  :
    • Hypothetical protein YbbP, contains nucleotide-binding domain of DisA bacterial checkpoint controller
  • PFAM:
    no clan defined DisA_N; DisA bacterial checkpoint controller nucleotide-binding (PF02457; HMM-score: 150.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00094
    • TAT(Tat/SPI): 0.000102
    • LIPO(Sec/SPII): 0.000511
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQLLKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFLKRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNISQELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEVSDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]