PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2115 [new locus tag: SAUSA300_RS11650 ]
• pan locus tag^?: SAUPAN005511000
• symbol: tnp
• pan gene symbol^?: tnp4
• synonym:
• product: IS1181, transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2115 [new locus tag: SAUSA300_RS11650 ]
• symbol: tnp
• product: IS1181, transposase
• replicon: chromosome
• strand: +
• coordinates: 2290382..2291620
• length: 1239
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914403 NCBI
• RefSeq: YP_494761 NCBI
• BioCyc: see SAUSA300_RS11650
• MicrobesOnline: 1293630 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  GTGACCAAACCCAAACTAAAGGAGACAAGTGCGCCTATGTGTAATGATACCTTAGAATTA
  CTAAGAATAAAAGATGAAAATATAAAATATATAAACCAAGAAATTGACGTCATTATCAAA
  GGAAAAAAAGCAACAGTGGTTAATGCTGTACTAACGTATAAGCCTTCGGCCTGTTATTGT
  TGTGGAGTTAAAAATGAAGGACAAATTCATAAACATGGTAAGCGTGTTTCTCGTATTACT
  TTACTTAAAACTCAAGGGTATAACACATACCTCAACTTAGCTAAACAACGTTTTAAATGC
  TTAGAATGCAATGGCACTTTTACTGCTAAAACGTCAATTGTTGATGAGTCGTGTTTTATC
  TCAAGATGTGTTACTCAAAAAGTTATAGAAGAAGCTACTAAAGTTAAAACAGAGATTGAT
  ACTGCAGAAGATAACTGTATCTCTCCATCTACTGTAAGTCGTATTAGAACTAAAGCGGCT
  AATTCATTACGAATTAAACCCTTTAATTGTTTGCCAGAACACATCGCTATGGATGAATTT
  AAAAGCGTTAAAAATGTAACTGGATCAATGAGTTTCATTTTTATAGATAATGATACTCAT
  GATGTTATAGATATTTTAGAAAATAGAACTACAAGATTCTTGCGTGCCTATTTCGAGCGA
  TTCGATTTAAAAAATCGACAACAAGTTAAGACGGTTACTATTGACATGTATGAACCCTAT
  GTCCGATTATTTCGCGACCTATTTCCTAATGCAGCTATTATTTTTGACAGATTCCATATC
  GTTCAACATTTAAATAGAGAACTTAATAAGTATCGTGTACAAGTTATGAATGAATACCGT
  AATAAAAAAGGACCTGATTATACAATTTTTAAGAATAACTGGAAAGTCCTATTGATGGAT
  ACTAGTAAAACCATATTTAGTAAATACAGATGGAATAAATCTTTTAAGGCTTATAAACGC
  TCATCTGACATTGTAGAATTCATGCTTTCAAAAGACGATATACTACGACACTCCTACGAA
  CTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGT
  TTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTAT
  AGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCT
  ATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_2115 [new locus tag: SAUSA300_RS11650 ]
• symbol: Tnp
• description: IS1181, transposase
• length: 412
• theoretical pI: 10.2563
• theoretical MW: 48326.9
• GRAVY: -0.486893

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.8)
  Metabolism Central intermediary metabolism One-carbon metabolism S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.8)
• TheSEED  :
  • Mobile element protein
• PFAM:
  RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 144.9)
  and 4 more
  Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 42.5)
  HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.7)
  no clan defined DUF3795; Protein of unknown function (DUF3795) (PF12675; HMM-score: 10.4)
  DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.048614
  • TAT(Tat/SPI): 0.001594
  • LIPO(Sec/SPII): 0.005897
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161892 NCBI
• RefSeq: YP_494761 NCBI
• UniProt: A0A0H2XI05 UniProt

⊟Protein sequence

• MTKPKLKETSAPMCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPSACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIVDESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLPEHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVKTVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYTIFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLRKDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications