PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2146 [new locus tag: SAUSA300_RS11825 ]
• pan locus tag^?: SAUPAN005582000
• symbol: SAUSA300_2146
• pan gene symbol^?: —
• synonym:
• product: alcohol dehydrogenase, zinc-containing

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2146 [new locus tag: SAUSA300_RS11825 ]
• symbol: SAUSA300_2146
• product: alcohol dehydrogenase, zinc-containing
• replicon: chromosome
• strand: -
• coordinates: 2322717..2323718
• length: 1002
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914517 NCBI
• RefSeq: YP_494782 NCBI
• BioCyc: see SAUSA300_RS11825
• MicrobesOnline: 1293661 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAATGATAGGATTTGAGAAGCCCTTTAAATTGGAAGAAGGAAATTTATTTAAAGTA
  TACGAACAAAGAAAGCCAACGCCAGAAAACGATGATATCTTAGTCAAAGTAAATAGCATT
  AGTGTAAATCCTGTAGATACAAAGCAACGACAAATGGAAGTCACTCAAGCACCTCGTGTA
  CTTGGGTTTGATGCTATTGGTACTGTTGAAGCTATCGGACCAGACGTTACATTATTTAGT
  CCGGGTGACGTTGTATTTTATGCTGGATCGCCAAATAGACAAGGATCAAATGCGACATAT
  CAATTGGTGTCAGAAGCTATAGTTGCAAAAGCACCACACAACATAAGTGCTAATGAAGCG
  GTTAGTCTACCGCTAACTGGTATTACGGCATATGAAACGTTTTTTGATACATTTAAAATT
  TCTACGAATCCTGCTGAAAACGAAGGGAAGTCTGTCTTAATCATTAATGGTGCCGGTGGT
  GTAGGCAGTATAGCCACTCAAATTGCTAAACGGTATGGTTTAACTGTCATTACAACAGCA
  TCACGACAAGAAACAACAGAGTGGTGTGAAAAGATGGGCGCAGATATCGTATTGAATCAT
  AAAGAGGATTTAGTGCGTCAATTTAAAGAAATTCCATTAGTAGATTATATATTTTGCACC
  TATAATACAGACTTGTATTACAATACTATGATTGAACTGATAAAACCATTAGGGCATATT
  ACGACTATCGTAGCTTTTAATGAAGATCAAGATTTAAATGCATTAAAATTAAAAAGTATA
  ACATTTACACATGAATTTATGTTTGCGCGTCCAATTCATCGAACGCCTGATATGATTAAA
  CAACATGAGTATTTAGAAGATATTACGAAAAATATAGAATCAGGGCATTACCAACCAACA
  ACGACACAAGTTTTTGAAGGTTTATCACCTGAAAACTTATATCAAGCACATCAATTGTTG
  GAAAAACAATCAATGATTGGAAAATTAGTAATTAATATATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_2146 [new locus tag: SAUSA300_RS11825 ]
• symbol: SAUSA300_2146
• description: alcohol dehydrogenase, zinc-containing
• length: 333
• theoretical pI: 5.09697
• theoretical MW: 37269.2
• GRAVY: -0.221922

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 326.8)
  and 14 more
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 138.3)
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 70.4)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 68.9)
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 39.1)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)mycothiol dehydrogenase (TIGR03451; EC 1.1.1.306; HMM-score: 38.1)
  leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 36.9)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 32.6)
  Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 32.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 28.5)
  putative phosphonate catabolism associated alcohol dehydrogenase (TIGR03366; HMM-score: 20)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 19.7)
  Metabolism Energy metabolism Fermentation S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 19.7)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02822; EC 1.-.-.-; HMM-score: 18.9)
  acetyl coenzyme A synthetase (ADP forming), alpha domain (TIGR02717; EC 6.2.1.13; HMM-score: 14.8)
• TheSEED  :
  • Bifunctional protein: zinc-containing alcohol dehydrogenase
  • quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-)
  • Similar to arginate lyase
• PFAM:
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 54.2)
  ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 49.2)
  and 4 more
  GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 29.4)
  NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 17.7)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 13.1)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006295
  • TAT(Tat/SPI): 0.000259
  • LIPO(Sec/SPII): 0.000768
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160165 NCBI
• RefSeq: YP_494782 NCBI
• UniProt: A0A0H2XEE2 UniProt

⊟Protein sequence

• MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNPVDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGGVGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications