PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2149 [new locus tag: SAUSA300_RS11840 ]
• pan locus tag^?: SAUPAN005585000
• symbol: lacG
• pan gene symbol^?: lacG
• synonym:
• product: 6-phospho-beta-galactosidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2149 [new locus tag: SAUSA300_RS11840 ]
• symbol: lacG
• product: 6-phospho-beta-galactosidase
• replicon: chromosome
• strand: -
• coordinates: 2326371..2327783
• length: 1413
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915253 NCBI
• RefSeq: YP_494785 NCBI
• BioCyc: see SAUSA300_RS11840
• MicrobesOnline: 1293664 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTAAAACATTACCTGAAGATTTTATTTTTGGTGGAGCAACAGCAGCATATCAAGCA
  GAAGGTGCGACAAATACGGATGGTAAAGGTCGCGTAGCATGGGATACGTATTTAGAAGAA
  AACTATTGGTACACTGCAGAACCAGCGAGTGATTTTTATAACAGATATCCAGTGGATTTA
  GAATTAAGTGAAAAATTCGGTGTTAATGGTATCCGAATTTCAATTGCATGGTCTCGTATC
  TTCCCAAATGGATATGGCGAAGTTAACCCTAAAGGTGTTGAGTATTACCATAAGTTATTT
  GCAGAGTGTCATAAACGTCATGTTGAACCATTTGTAACATTGCATCATTTTGATACACCA
  GAAGTGTTACACAAAGATGGCGATTTTCTAAATCGTAAAACGATTGACTACTTTGTAGAT
  TATGCTGAATATTGTTTTAAAGAATTCCCAGAAGTAAAATATTGGACTACATTCAATGAA
  ATTGGACCTATTGGTGATGGTCAATATTTAGTAGGTAAATTCCCTCCAGGTATTAAATAT
  GATTTTGAAAAAGTATTCCAATCACATCATAACATGATGGTGGCTCATGCTAGAGCAGTA
  AAATTATTTAAAGATGGCGGATACAAAGGAGAAATTGGTGTTGTACACGCATTGCCAACA
  AAATATCCGTTCGATCCATCAAATCCTGAAGATGTTCGTGCAGCAGAATTAGAAGATATC
  ATCCATAATAAATTCATCTTAGATGCAACATATTTAGGTAAGTATTCTCGTGAAACGATG
  GAAGGTGTGCAACATATCTTATCTGTTAATGGCGGTAAATTAAACATTACAGATGAAGAT
  TATGCCATTTTAGATGCAGCCAAAGATTTAAACGACTTCTTAGGTATCAATTACTACATG
  AGTGATTGGATGAGAGGTTACGATGGAGAATCTGAAATCACGCATAATGCGACAGGTGAT
  AAAGGCGGTTCTAAATACCAACTTAAAGGTGTCGGACAACGTGAATTTGATGTCGACGTA
  CCTCGCACAGACTGGGACTGGATGATCTATCCTCAAGGTTTATATGATCAAATCATGCGT
  GTTGTTAAAGATTATCCTAACTATCATAAGATTTATATCACTGAAAATGGTTTAGGATAT
  AAAGATGAATTTATTGAATCTGAAAAAACAGTTCATGATGATGCACGTATTGATTATGTA
  AGACAACATTTGAACGTTATTGCAGATGCTATTATAGATGGTGCAAATGTTAAAGGTTAC
  TTTATTTGGTCATTAATGGATGTGTTCTCTTGGTCAAATGGTTATGAAAAACGATATGGT
  TTATTCTATGTCGATTTTGAGACTCAAGAACGTTATCCTAAGAAAAGTGCTTATTGGTAC
  AAAGAGTTAGCCGAAACAAAAGAAATTAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2149 [new locus tag: SAUSA300_RS11840 ]
• symbol: LacG
• description: 6-phospho-beta-galactosidase
• length: 470
• theoretical pI: 4.91432
• theoretical MW: 54549.6
• GRAVY: -0.577447

⊟Function

• reaction:
  EC 3.2.1.85?  ExPASy
  6-phospho-beta-galactosidase A 6-phospho-beta-D-galactoside + H₂O = 6-phospho-D-galactose + an alcohol
• TIGRFAM:
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 6-phospho-beta-galactosidase (TIGR01233; EC 3.2.1.85; HMM-score: 865.7)
  and 1 more
  beta-galactosidase (TIGR03356; HMM-score: 389.6)
• TheSEED  :
  • 6-phospho-beta-galactosidase (EC 3.2.1.85)
  Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  6-phospho-beta-galactosidase (EC 3.2.1.85)
• PFAM:
  Glyco_hydro_tim (CL0058) Glyco_hydro_1; Glycosyl hydrolase family 1 (PF00232; HMM-score: 585)
  and 1 more
  C2 (CL0154) IcmF_C; Type VI secretion protein IcmF C-terminal (PF06744; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.093604
  • TAT(Tat/SPI): 0.025265
  • LIPO(Sec/SPII): 0.018078
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161282 NCBI
• RefSeq: YP_494785 NCBI
• UniProt: Q2FEU3 UniProt

⊟Protein sequence

• MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEIGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: lacG < lacE < lacF < lacD < lacC < lacB < lacA

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications