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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2151 [new locus tag: SAUSA300_RS11850 ]
  • pan locus tag?: SAUPAN005587000
  • symbol: lacF
  • pan gene symbol?: lacF
  • synonym:
  • product: PTS system, lactose-specific IIA component

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2151 [new locus tag: SAUSA300_RS11850 ]
  • symbol: lacF
  • product: PTS system, lactose-specific IIA component
  • replicon: chromosome
  • strand: -
  • coordinates: 2329519..2329830
  • length: 312
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGAATAGAGAAGAAGTCCAATTATTAGGTTTTGAAATTGTTGCATTTGCAGGGGATGCA
    CGTTCTAAGTTTTTAGAAGCATTAACAGCAGCTCAAGCTGGAGATTTTGCAAAAGCAGAT
    GCACTGATTGAAGAAGGAAACAATTGCATTGCGGAAGCGCATAGAGCACAAACAAGCCTG
    TTGGCTAAAGAAGCGCAAGGTGATGATATTGCATACAGTGTAACGATGATGCATGGTCAA
    GATCATTTAATGACAACAATTTTACTGAAAGATTTAATGAAGCATTTATTAGAATTTTAT
    AAAAGAGGGTGA
    60
    120
    180
    240
    300
    312

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2151 [new locus tag: SAUSA300_RS11850 ]
  • symbol: LacF
  • description: PTS system, lactose-specific IIA component
  • length: 103
  • theoretical pI: 4.85685
  • theoretical MW: 11369.9
  • GRAVY: -0.0990291

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.69?  ExPASy
    Transferred entry: 2.7.1.191, 2.7.1.192, 2.7.1.193, 2.7.1.194, 2.7.1.195, 2.7.1.196, 2.7.1.197, 2.7.1.198, 2.7.1.199, 2.7.1.200, 2.7.1.201, 2.7.1.202, 2.7.1.203, 2.7.1.204, 2.7.1.205, 2.7.1.206, 2.7.1.207 and 2.7.1.208
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose-specific IIa component (TIGR00823; EC 2.7.1.69; HMM-score: 157.8)
    and 1 more
    antimicrobial peptide, SdpC family (TIGR04032; HMM-score: 13.1)
  • TheSEED  :
    • PTS system, lactose-specific IIA component (EC 2.7.1.207)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  PTS system, lactose-specific IIA component (EC 2.7.1.69)
  • PFAM:
    no clan defined PTS_IIA; PTS system, Lactose/Cellobiose specific IIA subunit (PF02255; HMM-score: 111.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.047697
    • TAT(Tat/SPI): 0.120458
    • LIPO(Sec/SPII): 0.004871
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIEEGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKRG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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