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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2264 [new locus tag: SAUSA300_RS12510 ]
  • pan locus tag?: SAUPAN005795000
  • symbol: SAUSA300_2264
  • pan gene symbol?: rpiRc
  • synonym:
  • product: RpiR family phosphosugar-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2264 [new locus tag: SAUSA300_RS12510 ]
  • symbol: SAUSA300_2264
  • product: RpiR family phosphosugar-binding transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 2433701..2434582
  • length: 882
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGATTAATATGTCAAACGTACTAACAGAAATAGATAGTCAATATCCATATATGACTAAA
    AATGAAAAAAAGATAGCTAAATTTATACTAAATTCACCTCAAAAGGTGATTAAAATGAGG
    TCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAG
    AAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAA
    GCATCATCAATTTACAATGTTGAATTAATGAATAACGAAAGCACAGAATCTTTAAGAACT
    AAACTCCATACACGTACTACACGCGCACTTAATCATGCAAATAACGAATTAAATGATAAA
    ACTATTGATCAAATATGTCATTGTTTAAAACGTTCTGAGACGATTTTTATATATGGTTTT
    GGCGCATCTTTTGTAGTTGCTACCGACTTATACCAAAAGTTATCAAGAATAGGTCTTAAT
    ATTCAACTTGTTCAAGAAACACATATTTTTGCTACATTATTAGCAACTCACAATTCGAAC
    GACAGTGTTATTCTTATTACTAACAATGGTACGCAAAGTGAAATGCAATCAATGGTTAAA
    GTTATTGATGACTACCATATACCTATAATTACAATTACTAGTACAAGGGATAATCCCGTA
    GCACAGGCATCAAATATTGTTTTAACTTATGGGAAAACTGATGAAAATGAGATGCATATG
    GGAGCAACAACTTCATTATTTGCTCAAATGTTCACTATTGATATTTTATATTATCGTTAT
    GTTGCATTAAATTATCACGCATCATTAGATTTCATAACACAATCTAAAATGGCTTTAGAT
    AACTATCGTAAACATTTATCAAATATTAACTTTAAACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2264 [new locus tag: SAUSA300_RS12510 ]
  • symbol: SAUSA300_2264
  • description: RpiR family phosphosugar-binding transcriptional regulator
  • length: 293
  • theoretical pI: 8.87992
  • theoretical MW: 33363
  • GRAVY: -0.233788

⊟Function[edit | edit source]

  • TIGRFAM:
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 40.9)
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 35.5)
    and 2 more
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 16.4)
    phosphoglycerol geranylgeranyltransferase (TIGR04146; EC 2.5.1.41; HMM-score: 13.7)
  • TheSEED  :
    • Sialic acid utilization regulator, RpiR family
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Sialic acid utilization regulator, RpiR family
  • PFAM:
    HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 70.4)
    SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 63.6)
    and 4 more
    DUF2529; Domain of unknown function (DUF2529) (PF10740; HMM-score: 15.5)
    SIS_2; SIS domain (PF13580; HMM-score: 14.8)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 14.1)
    CheY (CL0304) FleQ; Flagellar regulatory protein FleQ (PF06490; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001725
    • TAT(Tat/SPI): 0.00011
    • LIPO(Sec/SPII): 0.000179
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINMSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASLLDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTRTTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQLVQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVAQASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMALDNYRKHLSNINFKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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