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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2294 [new locus tag: SAUSA300_RS12675 ]
  • pan locus tag?: SAUPAN005847000
  • symbol: SAUSA300_2294
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2294 [new locus tag: SAUSA300_RS12675 ]
  • symbol: SAUSA300_2294
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2466726..2467217
  • length: 492
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGTTATTATCAAACAACTTTTTACACATACTCAAACCGTAACATCTGAATTCATTGAC
    CATAACAATCATATGCATGATGCAAATTATAATATCATTTTTAGTGACGTCGTGAATCGT
    TTTAATTACAGCCACGGTCTTTCTTTAAAAGAACGCGAAAATTTAGCATATACGCTATTT
    ACACTAGAAGAACATACGACATACCTCTCAGAATTGTCTCTTGGCGATGTATTTACTGTT
    ACTTTATATATTTATGATTACGATTATAAGCGGTTGCATTTATTTTTAACATTAACTAAA
    GAAGATGGTACACTAGCATCAACAAATGAAGTAATGATGATGGGAATTAATCAGCACACA
    CGTCGTTCTGATGCTTTTCCTGAATCATTTTCAACACAAATAGCACACTATTATAAAAAT
    CAATCAACTATCACTTGGCCTGAACAATTAGGACATAAAATAGCAATTCCACACAAAGGA
    GCATTAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2294 [new locus tag: SAUSA300_RS12675 ]
  • symbol: SAUSA300_2294
  • description: hypothetical protein
  • length: 163
  • theoretical pI: 6.58504
  • theoretical MW: 19024.3
  • GRAVY: -0.365644

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General acyl-CoA thioester hydrolase, YbgC/YbaW family (TIGR00051; HMM-score: 17.2)
    and 1 more
    tol-pal system-associated acyl-CoA thioesterase (TIGR02799; EC 3.1.2.-; HMM-score: 13.7)
  • TheSEED  :
    • 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
    Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
    and 3 more
    Amino Acids and Derivatives Branched-chain amino acids Valine degradation  3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
    Carbohydrates Fermentation Acetyl-CoA fermentation to Butyrate  3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
    Fatty Acids, Lipids, and Isoprenoids Fatty Acids, Lipids, and Isoprenoids - no subcategory Polyhydroxybutyrate metabolism  3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
  • PFAM:
    HotDog (CL0050) 4HBT_2; Thioesterase-like superfamily (PF13279; HMM-score: 57.3)
    and 2 more
    4HBT; Thioesterase superfamily (PF03061; HMM-score: 15.4)
    no clan defined Tmpp129; Putative transmembrane protein precursor (PF10272; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002901
    • TAT(Tat/SPI): 0.000141
    • LIPO(Sec/SPII): 0.000476
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYSHGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKEDGTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKGALK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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