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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2346 [new locus tag: SAUSA300_RS12950 ]
  • pan locus tag?: SAUPAN005932000
  • symbol: nirB
  • pan gene symbol?: nasD
  • synonym:
  • product: nitrite reductase [NAD(P)H], large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2346 [new locus tag: SAUSA300_RS12950 ]
  • symbol: nirB
  • product: nitrite reductase [NAD(P)H], large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2522076..2524481
  • length: 2406
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAAAGCAAAAACTAGTAATGATTGGTAACGGTATGGCGGGCATTCGAACAATCGAA
    GAAATATTAGAGCGCGCCAACGATTTATATGATATTACGGTAATAGGTAAAGAACCTTAT
    CCAAACTATAACCGCATCATGCTTTCAAATATTTTACAAAATAAAATGACAGTTGAAGAA
    ACAATTATGAATCCATATGAGTGGTATGAGGAACATGGTATCGAGTTAATTACAAATGAT
    CCTGTAATTGAAGTAGATAGAGCAAATCAATCAGTTACTACTGCAAATGGTATTGAAGTA
    TCATACGATAAATTGATTTTTGCGACAGGTTCTAAAGCATTCGTCATTCCAGTTCCAGGT
    TCGACATTACCGAGTGTAATTGGTTGGAGAACGCTTGATGATACTGAACAAATGATGAAC
    ATTGCTAAGACTAAAAAGAAAGCAATTGTAATTGGTGGAGGATTACTAGGTTTAGAGTGT
    GCACGTGGTTTATTAGATCAAGGTATGGAAGTAACGGTGTTACATTTAGCTGAATGGTTG
    ATGGAAATGCAACTAGACCGTAAAGCTGGAAATATGCTTAAAGCAGATCTAGAAAAGCAA
    GGTATGAAGTTTGAAATGCAAGCCAACACAACTGAAATCTTAGGAGAAGATGATGTTGAG
    GGTGTTAAATTGGCTGATGGACGCGAGATTCCGGCAGACTTAGTTGTTATGGCAGTAGGT
    ATACGACCATACACAGAAGTAGCCAAAGAATCAGGTCTAGATGTTAATCGTGGTATTGTT
    GTCAATGATGTGATGCAAACAAGTGATAGCAATGTATATGCAGTTGGTGAATGTGCAGAA
    CATAACGGCAAAGTTTATGGACTCGTTGCACCACTATATGAACAAGGTAAAGTATTAGCT
    GATCATTTAACAAATAAAGAAACGAACGGATACAAGGGATCAACAACATTTACGTCATTA
    AAAGTTTCTGGGTGTGACTTGTATAGTGCTGGTCAAATTGTAGAAAATGCAGAAATTAAA
    GGTATTGAAATATTTAATAGTGTTGATAATAACTATAAAAAAATCTTTTTAAAAGACGGT
    AATGTAGTTGGTGCAGTACTATATGGTGATATCGATGATGGTTCACGCTTTTATAACATG
    ATGAAAAAAGGTGAATCCACTGAAGATTACACACTTGTATCATTGCTTACTAAAGGTGGA
    GAAGAGGCATCGCTATCAATTGCTGATATGGCTGATGATGAAACAATTTGTGGTTGTAAT
    GGTGTTGATAAAGGTACTATAGTAAATGCGATTACGGAAAATGGCTTTACAACAGTTGAA
    GAAGTAACGGCTAAAACAAAAGCGGGGAATTCATGTGGTAAATGTAAACCGCAAATTGCT
    CAAATATTGCAGCACACCTTAGGAGATGACTTTGTTGCCGCAAAACCTGCTGGTATATGT
    GGTTGTACTGATTTGACACGCGATCAAATAGTAACGCAAATAAGAGCGAAAGGTTTAAAA
    ACATCTAAAGAAGTTCGACATGTTTTAAACTTTAAAAATAAAGGTGGATGTCCAAAATGT
    CGACCAGCAATCAACTATTATTTAAACATGGTTTATCCACATGATCATGAAGATGAAAGA
    GAATCAAGATTTGCTAACGAACGTTACCATGCGAATATTCAAAATGATGGTACATTTTCT
    GTTATACCTCAAATGCGTGGGGGTGTTACAGATGCAGACCAACTGATTCGTCTAGGAGAA
    GTGGCTAAGAAATATCATGTGCCACTAGTTAAAGTGACAGGTTCACAACGTGTTGGTTTG
    TATGGAGTTAAAAAAGAAGAATTACCAAATATATGGGAAGACCTAGGTATGCGTTCAGCA
    TCAGCTTATGGTAAGAAAACACGCTCAGTTAAAAGCTGTGTTGGTAAAGAGTTTTGTCGA
    TTTGGTACGCAATACACGACACGACTTGGCATTCGTTTAGAAAAAACATTTGAATACATC
    GATACACCTCATAAATTCAAAATGGGTGTATCAGGCTGCCCAAGAAGTTGTGTTGAATCA
    GGTGTTAAAGATTTTGGTATTATCTCAGTTGAAAATGGATTCCAAATCTATATCGGTGGT
    AATGGTGGTACAGAAGTTGAAAAAGCTGAATTTTTAACAACTGTAGAAACAGAAGATGAA
    GTAATCAAATTATGTGGTGCTTTGATGCAATATTATCGCGAAACAGGTATATATGCTGAA
    AGAACAGCGCCATGGTTAAGAAGACTTGGATTTGAAAATGTAAAAGAAGTCTTACTTGAC
    CCAGAAAGACAAAATGAACTATTTGAACGCATTATGGATGCTAAAAAAGCAGTCGAAGCT
    GAACCTTGGGAAGCAATAACTAGCAATGCGCAAGCACGTAGAATTTTTGAAGTGGAGAAG
    GTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2406

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2346 [new locus tag: SAUSA300_RS12950 ]
  • symbol: NirB
  • description: nitrite reductase [NAD(P)H], large subunit
  • length: 801
  • theoretical pI: 4.99932
  • theoretical MW: 88656.5
  • GRAVY: -0.314107

⊟Function[edit | edit source]

  • reaction:
    EC 1.7.1.4?  ExPASy
    Nitrite reductase (NAD(P)H) Ammonia + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H
  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 929.9)
    and 34 more
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 144.2)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 99.3)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 80.1)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 68.3)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 57.3)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 56)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other Fe-S cluster assembly protein NifU (TIGR02000; HMM-score: 53.1)
    Metabolism Central intermediary metabolism Nitrogen fixation Fe-S cluster assembly protein NifU (TIGR02000; HMM-score: 53.1)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 49.8)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, subunit C (TIGR02912; EC 1.8.-.-; HMM-score: 48.2)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, dissimilatory-type alpha subunit (TIGR02064; EC 1.8.99.3; HMM-score: 48)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 47.2)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 34.3)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, ferredoxin dependent (TIGR02042; EC 1.8.7.1; HMM-score: 32.9)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 31.7)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 26.4)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase (NADPH) hemoprotein, beta-component (TIGR02041; EC 1.8.1.2; HMM-score: 25)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B synthase (TIGR02435; EC 1.14.13.83; HMM-score: 23.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 22.9)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 21.4)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 20.1)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 19.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 18.7)
    oxidoreductase/SelD-related fusion protein (TIGR04369; HMM-score: 18.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 17.3)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 16.9)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 15.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 14.7)
    lycopene cyclase family protein (TIGR01790; HMM-score: 14.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other carotene isomerase (TIGR02730; EC 5.-.-.-; HMM-score: 13.8)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, A subunit (TIGR03377; EC 1.1.5.3; HMM-score: 13.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 13.3)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 13)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 11.7)
  • TheSEED  :
    • Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 193.8)
    and 15 more
    no clan defined Fer2_BFD; BFD-like [2Fe-2S] binding domain (PF04324; HMM-score: 109.8)
    NIR_SIR; Nitrite and sulphite reductase 4Fe-4S domain (PF01077; HMM-score: 89.8)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 66.1)
    no clan defined NIR_SIR_ferr; Nitrite/Sulfite reductase ferredoxin-like half domain (PF03460; HMM-score: 43.4)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 40.5)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 27.9)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 25.5)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 24.5)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 22.5)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 16.2)
    Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 15.7)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 14.9)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 13.9)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 12.4)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007572
    • TAT(Tat/SPI): 0.000186
    • LIPO(Sec/SPII): 0.001205
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLECARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEVEKV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NreC* (activation) regulon, Rex (repression) regulon
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    Rex(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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