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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2397 [new locus tag: SAUSA300_RS13275 ]
  • pan locus tag?: SAUPAN006008000
  • symbol: SAUSA300_2397
  • pan gene symbol?:
  • synonym:
  • product: putative transport protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2397 [new locus tag: SAUSA300_RS13275 ]
  • symbol: SAUSA300_2397
  • product: putative transport protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2582237..2583424
  • length: 1188
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    TTGTCAATCATGCGACTATTTACATTCATTTTAAGTATTTTTATCGTAGGAATGGTTGAA
    ATGATGGTTGCAGGAATTATGAACTTGATGAGTCAGGACTTACATGTATCAGAAGCTGTC
    GTTGGTCAATTAGTGACAATGTACGCTTTAACATTTGCGATATGTGGACCTATTCTGGTT
    AAATTAACGAACCGTTTTTCATCAAGGCCTGTATTATTATGGACATTACTTATATTTATC
    ATTGGTAATGGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATT
    ATCTCATCTGCAGCAGCAGCACTAATTATCGTAAAAGTATTAGCTATTACAGCGATGTTA
    TCAGCACCTAAAAATCGTGGTAAAATGATTGGACTTGTCTATACAGGGTTTAGTGGTGCT
    AATGTTTTTGGTGTACCAATTGGAACGGTTATCGGCGATTTAGTAGGTTGGCGCTATACA
    TTTCTATTCTTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGATGATCTATTTACCG
    AAGGATCAGGAAATACAACGAGGCCCTGTGAATCATGAGACACCATCTCATGAAAATCAT
    GTTACTTCGAAAATATTAAGACCTGCTGAAGTAGCAAAATATTTAATTATTACGTTTTTA
    GTATTGATTGCAAACTCAGTGACATTCGTCTTTATAAATCCACTTATTTTATCTAATGGT
    CATGATATGTCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGA
    ACATCATTAGGTGGTATATTCTCCGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCT
    GTTTCTATTTTTATCGTCATGATGTTACTTATGAATTTAATCTTACCTGGTTCAGGTCTA
    TTGTTAGCAGGACTATTTATTTGGAATATCATGCAATGGAGTACTAATCCAGCAGTGCAA
    AGCGGTGTGATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCT
    AGTTTAAACGCTGGTATTGGTGTTGGAGGCATTATTGGAGGCTTGGTCATGACACATGTT
    TCTGTTCAAGCTATCACATATACGAGTGCCATCATTGGCGCATTAGGATTAATCGTTGTT
    TTCACATTGAAAAATAATCATTATGCTAAAACATTTAAATCATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2397 [new locus tag: SAUSA300_RS13275 ]
  • symbol: SAUSA300_2397
  • description: putative transport protein
  • length: 395
  • theoretical pI: 10.1636
  • theoretical MW: 42574.9
  • GRAVY: 0.966076

Function[edit | edit source]

  • TIGRFAM:
    multidrug resistance protein (TIGR00880; HMM-score: 96.1)
    and 10 more
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 71.8)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.1)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 46.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 41.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 36)
    Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 25.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 24.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 22.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 9.2)
  • TheSEED  :
    • Chloramphenicol resistance protein
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 199)
    and 2 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 27.1)
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 12.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.146458
    • TAT(Tat/SPI): 0.001097
    • LIPO(Sec/SPII): 0.03271
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]