PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2397 [new locus tag: SAUSA300_RS13275 ]
• pan locus tag^?: SAUPAN006008000
• symbol: SAUSA300_2397
• pan gene symbol^?: —
• synonym:
• product: putative transport protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2397 [new locus tag: SAUSA300_RS13275 ]
• symbol: SAUSA300_2397
• product: putative transport protein
• replicon: chromosome
• strand: -
• coordinates: 2582237..2583424
• length: 1188
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914084 NCBI
• RefSeq: YP_495032 NCBI
• BioCyc: see SAUSA300_RS13275
• MicrobesOnline: 1293912 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  TTGTCAATCATGCGACTATTTACATTCATTTTAAGTATTTTTATCGTAGGAATGGTTGAA
  ATGATGGTTGCAGGAATTATGAACTTGATGAGTCAGGACTTACATGTATCAGAAGCTGTC
  GTTGGTCAATTAGTGACAATGTACGCTTTAACATTTGCGATATGTGGACCTATTCTGGTT
  AAATTAACGAACCGTTTTTCATCAAGGCCTGTATTATTATGGACATTACTTATATTTATC
  ATTGGTAATGGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATT
  ATCTCATCTGCAGCAGCAGCACTAATTATCGTAAAAGTATTAGCTATTACAGCGATGTTA
  TCAGCACCTAAAAATCGTGGTAAAATGATTGGACTTGTCTATACAGGGTTTAGTGGTGCT
  AATGTTTTTGGTGTACCAATTGGAACGGTTATCGGCGATTTAGTAGGTTGGCGCTATACA
  TTTCTATTCTTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGATGATCTATTTACCG
  AAGGATCAGGAAATACAACGAGGCCCTGTGAATCATGAGACACCATCTCATGAAAATCAT
  GTTACTTCGAAAATATTAAGACCTGCTGAAGTAGCAAAATATTTAATTATTACGTTTTTA
  GTATTGATTGCAAACTCAGTGACATTCGTCTTTATAAATCCACTTATTTTATCTAATGGT
  CATGATATGTCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGA
  ACATCATTAGGTGGTATATTCTCCGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCT
  GTTTCTATTTTTATCGTCATGATGTTACTTATGAATTTAATCTTACCTGGTTCAGGTCTA
  TTGTTAGCAGGACTATTTATTTGGAATATCATGCAATGGAGTACTAATCCAGCAGTGCAA
  AGCGGTGTGATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCT
  AGTTTAAACGCTGGTATTGGTGTTGGAGGCATTATTGGAGGCTTGGTCATGACACATGTT
  TCTGTTCAAGCTATCACATATACGAGTGCCATCATTGGCGCATTAGGATTAATCGTTGTT
  TTCACATTGAAAAATAATCATTATGCTAAAACATTTAAATCATCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2397 [new locus tag: SAUSA300_RS13275 ]
• symbol: SAUSA300_2397
• description: putative transport protein
• length: 395
• theoretical pI: 10.1636
• theoretical MW: 42574.9
• GRAVY: 0.966076

⊟Function

• TIGRFAM:
  multidrug resistance protein (TIGR00880; HMM-score: 96.1)
  and 10 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 71.8)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.1)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 46.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 41.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 36)
  Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 25.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 24.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 22.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 9.2)
• TheSEED  :
  • Chloramphenicol resistance protein
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 199)
  and 2 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 27.1)
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.146458
  • TAT(Tat/SPI): 0.001097
  • LIPO(Sec/SPII): 0.03271
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 87161435 NCBI
• RefSeq: YP_495032 NCBI
• UniProt: A0A0H2XH93 UniProt

⊟Protein sequence

• MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications