PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2433 [new locus tag: SAUSA300_RS13475 ]
• pan locus tag^?: SAUPAN006091000
• symbol: SAUSA300_2433
• pan gene symbol^?: pgcA
• synonym:
• product: phosphoglucomutase/phosphomannomutase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2433 [new locus tag: SAUSA300_RS13475 ]
• symbol: SAUSA300_2433
• product: phosphoglucomutase/phosphomannomutase family protein
• replicon: chromosome
• strand: +
• coordinates: 2618195..2620003
• length: 1809
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912907 NCBI
• RefSeq: YP_495067 NCBI
• BioCyc: see SAUSA300_RS13475
• MicrobesOnline: 1293948 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCATTTTCATACACATCTATTATATAATACTTGTGAAAAGTATTGTCTTGGGGCTGTG
  TTTTTTTACTTTTGGGGCGTATTTCTTTATAATTCATTACATAAATGTAAGGGCTTTAGT
  TTTCATGTTTTATTAAGTCTAACTGAGATTTTGAAAGGATGTTTAGCAACAATGGATAAA
  GAATTATGGATAGAACGAGCTAATGATAGTTTAGTTAAACATTTTTATGAGCAGCAATCT
  GACATTGAACAGCGAGAAGGTTTTGAAAGTAAATTAACATTTGGTACTGCGGGTATACGC
  GGAAAATTCGGTCTTGGTGAAGGTCGACTTAATAAGTTTACTATTGAAAAATTGGCATTA
  GGTTTAGCGCGTTATTTAAATGCCCAAACAAACAGTCCAACAATAGTCATTCATTATGAT
  ATTAGACATCTTTCAACTGAATTCGCCCAAATAATTGCTAATGTATTAGCAAACCATCAA
  ATAACAGTTTATTTACCTGATACATATAAAACGACACCGGAATTATCGTTCGCGGTGCGC
  AATCTTAATACTACTGCTGGCATTATGATTACAGCAAGTCATAATCCGAAAGACTATAAC
  GGCATCAAAGTATATGGTTCTGATGGTGCGCAATTATCGACTGATGCATCTGAGCTTGCA
  AGTCGTTATATCGAAGAAGTTGGCGATCCATTACAGATTGATATACCTATTTCTAAACAA
  AATACATCTTATATCAAGCCTTTTCCGAAATCTGTAACTGATGACTATATGAAACACATT
  CAAAATATGATTGGCTATATTCCTAAATCTGATTTACAAGTTGTGTTCACTAGTTTGCAT
  GGTACAAGTGTTCCGATTGTACCTGAGTTATTACAATCTTTGAATTTCAATCAATTTAAT
  CTTGTAGAAGCACAATGTAAACCTGATCCGAATTTCAGCTCAGTTCAAAGTGCTAATCCT
  GAAGATCATCGCGCTTTTGATCAAGCAGTCGAACTTGCAAATAAAAGTCATGCAGACTTA
  TTAATATCAACTGACCCTGATGCAGATCGACTTGGTATTGCTGAACGTGATGCACACGGT
  CATATCACATATTTTAATGGCAACCAAATTGGTGCGCTGTTACTTAATTATCGTATTCAA
  CAAACGTCTCAATTACGTCACCGCTTAATGATTCAATCTATTGTAAGTAGTGAGTTAACA
  AAGTCATTAGCACGCTATAATAATGTTGAATACAAAGAGGTGCTGACGGGCTTTAAATTT
  ATTGCTCAGGAAATAAGACAACTGGATGATCATCAAAATATGATTTTCGCATTTGAAGAG
  AGTTACGGTTTTCTATCAGAGCCTTTTGTACGTGATAAAGATGCCGTACAAATTGTGCCA
  CTCATTATAAAATACGCTTCTGAGTTAAAATTATATGGCAAAACATTGAAAGATGAATTA
  GAACAGATTTATCAAACTGTTGGTAGACATGAGGATACATTATTCTCGCATACGCTAGAA
  GGATTTGAAGGGAAAAAGAAAATTAATGCAATAATGACCAAATTCCGTTCAAATCCACCG
  CAAGAGATTCAAGGACTCAAAGTGAAAGCAATTGAAGATTATTTAACTAGCGAGGTTTAT
  CATTTGGATAAAGATACAACGTCGCAGATTAATTCTCCTAAATCAAATGTTATTCGTGTC
  TTATTTGACGAAGGATTTATCGCTTTACGTCCTTCTGGTACTGAACCTAAAATTAAATTA
  TATGTGTCGTTAAAATGTCCGAATTTTGATGATGTAGCTCAAAAAATTAATGCGATGATT
  TTTAGCTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2433 [new locus tag: SAUSA300_RS13475 ]
• symbol: SAUSA300_2433
• description: phosphoglucomutase/phosphomannomutase family protein
• length: 602
• theoretical pI: 6.55157
• theoretical MW: 68347.3
• GRAVY: -0.275581

⊟Function

• reaction:
  EC 5.4.2.2?  ExPASy
  Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
• TIGRFAM:
  Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR03990; EC 5.4.2.10; HMM-score: 171.6)
  and 3 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 97.5)
  Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 97.5)
  Metabolism Energy metabolism Sugars phosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR01132; EC 5.4.2.2; HMM-score: 74.1)
• TheSEED  :
  • Phosphoglucosamine mutase (EC 5.4.2.10)
  • Phosphomannomutase (EC 5.4.2.8)
  Carbohydrates Monosaccharides Mannose Metabolism  Phosphomannomutase (EC 5.4.2.8)
  and 2 more
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Phosphoglucosamine mutase (EC 5.4.2.10)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Phosphoglucosamine mutase (EC 5.4.2.10)
• PFAM:
  no clan defined PGM_PMM_I; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I (PF02878; HMM-score: 142.1)
  and 4 more
  PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 93.7)
  PGM_PMM_III; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III (PF02880; HMM-score: 56.7)
  PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 32.3)
  Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02324
  • TAT(Tat/SPI): 0.000711
  • LIPO(Sec/SPII): 0.005581
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87160054 NCBI
• RefSeq: YP_495067 NCBI
• UniProt: Q2FE11 UniProt

⊟Protein sequence

• MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVLLSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGKFGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASELASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVFTSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELANKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQSIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFVRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKINAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGFIALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications