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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2433 [new locus tag: SAUSA300_RS13475 ]
  • pan locus tag?: SAUPAN006091000
  • symbol: SAUSA300_2433
  • pan gene symbol?: pgcA
  • synonym:
  • product: phosphoglucomutase/phosphomannomutase family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2433 [new locus tag: SAUSA300_RS13475 ]
  • symbol: SAUSA300_2433
  • product: phosphoglucomutase/phosphomannomutase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2618195..2620003
  • length: 1809
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCATTTTCATACACATCTATTATATAATACTTGTGAAAAGTATTGTCTTGGGGCTGTG
    TTTTTTTACTTTTGGGGCGTATTTCTTTATAATTCATTACATAAATGTAAGGGCTTTAGT
    TTTCATGTTTTATTAAGTCTAACTGAGATTTTGAAAGGATGTTTAGCAACAATGGATAAA
    GAATTATGGATAGAACGAGCTAATGATAGTTTAGTTAAACATTTTTATGAGCAGCAATCT
    GACATTGAACAGCGAGAAGGTTTTGAAAGTAAATTAACATTTGGTACTGCGGGTATACGC
    GGAAAATTCGGTCTTGGTGAAGGTCGACTTAATAAGTTTACTATTGAAAAATTGGCATTA
    GGTTTAGCGCGTTATTTAAATGCCCAAACAAACAGTCCAACAATAGTCATTCATTATGAT
    ATTAGACATCTTTCAACTGAATTCGCCCAAATAATTGCTAATGTATTAGCAAACCATCAA
    ATAACAGTTTATTTACCTGATACATATAAAACGACACCGGAATTATCGTTCGCGGTGCGC
    AATCTTAATACTACTGCTGGCATTATGATTACAGCAAGTCATAATCCGAAAGACTATAAC
    GGCATCAAAGTATATGGTTCTGATGGTGCGCAATTATCGACTGATGCATCTGAGCTTGCA
    AGTCGTTATATCGAAGAAGTTGGCGATCCATTACAGATTGATATACCTATTTCTAAACAA
    AATACATCTTATATCAAGCCTTTTCCGAAATCTGTAACTGATGACTATATGAAACACATT
    CAAAATATGATTGGCTATATTCCTAAATCTGATTTACAAGTTGTGTTCACTAGTTTGCAT
    GGTACAAGTGTTCCGATTGTACCTGAGTTATTACAATCTTTGAATTTCAATCAATTTAAT
    CTTGTAGAAGCACAATGTAAACCTGATCCGAATTTCAGCTCAGTTCAAAGTGCTAATCCT
    GAAGATCATCGCGCTTTTGATCAAGCAGTCGAACTTGCAAATAAAAGTCATGCAGACTTA
    TTAATATCAACTGACCCTGATGCAGATCGACTTGGTATTGCTGAACGTGATGCACACGGT
    CATATCACATATTTTAATGGCAACCAAATTGGTGCGCTGTTACTTAATTATCGTATTCAA
    CAAACGTCTCAATTACGTCACCGCTTAATGATTCAATCTATTGTAAGTAGTGAGTTAACA
    AAGTCATTAGCACGCTATAATAATGTTGAATACAAAGAGGTGCTGACGGGCTTTAAATTT
    ATTGCTCAGGAAATAAGACAACTGGATGATCATCAAAATATGATTTTCGCATTTGAAGAG
    AGTTACGGTTTTCTATCAGAGCCTTTTGTACGTGATAAAGATGCCGTACAAATTGTGCCA
    CTCATTATAAAATACGCTTCTGAGTTAAAATTATATGGCAAAACATTGAAAGATGAATTA
    GAACAGATTTATCAAACTGTTGGTAGACATGAGGATACATTATTCTCGCATACGCTAGAA
    GGATTTGAAGGGAAAAAGAAAATTAATGCAATAATGACCAAATTCCGTTCAAATCCACCG
    CAAGAGATTCAAGGACTCAAAGTGAAAGCAATTGAAGATTATTTAACTAGCGAGGTTTAT
    CATTTGGATAAAGATACAACGTCGCAGATTAATTCTCCTAAATCAAATGTTATTCGTGTC
    TTATTTGACGAAGGATTTATCGCTTTACGTCCTTCTGGTACTGAACCTAAAATTAAATTA
    TATGTGTCGTTAAAATGTCCGAATTTTGATGATGTAGCTCAAAAAATTAATGCGATGATT
    TTTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1809

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2433 [new locus tag: SAUSA300_RS13475 ]
  • symbol: SAUSA300_2433
  • description: phosphoglucomutase/phosphomannomutase family protein
  • length: 602
  • theoretical pI: 6.55157
  • theoretical MW: 68347.3
  • GRAVY: -0.275581

Function[edit | edit source]

  • reaction:
    EC 5.4.2.2?  ExPASy
    Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
  • TIGRFAM:
    Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR03990; EC 5.4.2.10; HMM-score: 171.6)
    and 3 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 97.5)
    Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 97.5)
    Metabolism Energy metabolism Sugars phosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR01132; EC 5.4.2.2; HMM-score: 74.1)
  • TheSEED  :
    • Phosphoglucosamine mutase (EC 5.4.2.10)
    • Phosphomannomutase (EC 5.4.2.8)
    Carbohydrates Monosaccharides Mannose Metabolism  Phosphomannomutase (EC 5.4.2.8)
    and 2 more
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Phosphoglucosamine mutase (EC 5.4.2.10)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Phosphoglucosamine mutase (EC 5.4.2.10)
  • PFAM:
    no clan defined PGM_PMM_I; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I (PF02878; HMM-score: 142.1)
    and 4 more
    PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 93.7)
    PGM_PMM_III; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III (PF02880; HMM-score: 56.7)
    PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 32.3)
    Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02324
    • TAT(Tat/SPI): 0.000711
    • LIPO(Sec/SPII): 0.005581
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVLLSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGKFGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASELASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVFTSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELANKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQSIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFVRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKINAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGFIALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]