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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2476 [new locus tag: SAUSA300_RS13740 ]
  • pan locus tag?: SAUPAN006195000
  • symbol: ptsG
  • pan gene symbol?: glcB
  • synonym:
  • product: phosphotransferase system, glucose-specific IIABC component

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2476 [new locus tag: SAUSA300_RS13740 ]
  • symbol: ptsG
  • product: phosphotransferase system, glucose-specific IIABC component
  • replicon: chromosome
  • strand: -
  • coordinates: 2672276..2674342
  • length: 2067
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTTTAAGAAATTGTTTGGACAATTGCAACGTATCGGTAAAGCATTAATGTTACCTGTT
    GCGATTTTACCAGCAGCTGGTATTTTATTAGCGTTTGGTAACGCAATGCACAACGAACAA
    TTAGTAGAAATTGCACCATGGTTAAAAAACGATATCATTGTAATGATTTCGTCGGTCATG
    GAAGCAGCAGGACAAGTTGTATTTGATAACTTGCCATTATTATTTGCAGTTGGTACAGCA
    CTTGGATTAGCAGGAGGAGACGGTGTTGCAGCATTAGCAGCGCTAGTAGGTTACTTAATT
    ATGAATGCAACAATGGGGAAAGTGTTGCACATTACAATTGATGACATTTTCTCATATGCC
    AAAGGGGCAAAAGAATTAAGTCAAGCAGCGAAAGAACCAGCACATGCTTTAGTATTAGGT
    ATTCCAACGTTACAAACGGGTGTGTTTGGTGGTATTATCATGGGTGCTTTAGCCGCATGG
    TGTTACAACAAATTTTATAATATTACACTACCACCATTTTTAGGATTCTTTGCAGGTAAA
    CGATTTGTACCGATTGTGACATCGGTCGTAGCAATCGCAACAGGTGTGCTTTTAAGCTTT
    GCGTGGCCACCAATTCAAGATGGATTAAATAGTTTATCGAATTTCTTATTAAATAAAAAT
    TTAACATTAACAACGTTTATATTCGGTATTATTGAACGCTCATTAATTCCATTTGGTTTA
    CATCATATTTTCTATTCACCGTTCTGGTTTGAATTCGGAAGTTATACAAATCACGCAGGT
    GAATTGGTTCGTGGTGACCAACGTATTTGGATGGCACAATTGAAAGATGGCGTACCATTT
    ACTGCTGGTGCATTTACTACTGGTAAATATCCATTTATGATGTTTGGTTTACCAGCGGCG
    GCATTTGCTATTTATAAAAATGCACGACCAGAACGTAAAAAAGTCGTGGGTGGTTTAATG
    TTATCAGCAGGATTAACTGCATTTTTAACTGGTATCACTGAGCCATTAGAATTTTCATTC
    TTATTTGTAGCACCAGTACTTTATGGAATTCACGTATTATTAGCTGGTACATCATTCTTA
    GTAATGCATTTATTAGGCGTTAAAATTGGTATGACATTCTCAGGTGGTTTCATAGATTAT
    ATTTTATATGGTTTATTAAACTGGGATCGTTCACACGCATTATTAGTTATTCCAGTCGGT
    ATTGTATATGCTATCGTGTATTACTTCTTATTCGACTTTGCAATTCGTAAGTTTAAATTG
    AAAACACCAGGTCGTGAAGATGAAGAAACTGAAATTCGTAACTCTAGTGTCGCAAAATTA
    CCATTTGATGTCTTAGATGCAATGGGTGGAAAAGAAAACATTAAACATTTAGATGCATGT
    ATTACACGTCTACGCGTAGAAGTGGTTGATAAATCAAAAGTAGATGTAGCAGGTATTAAA
    GCTTTAGGCGCATCAGGTGTATTAGAAGTTGGAAACAATATGCAAGCTATCTTTGGTCCA
    AAATCAGATCAAATTAAACATGATATGGCCAAGATTATGAGTGGTGAAATTACGAAACCA
    AGTGAAACGACAGTGACTGAAGAAATGTCAGATGAACCAGTTCACGTAGAAGCACTTGGA
    ACAACAGACATCTATGCACCAGGTATCGGTCAAATCATTCCATTATCAGAAGTACCTGAT
    CAAGTGTTCGCTGGTAAAATGATGGGTGATGGTGTTGGCTTTATCCCTGAAAAAGGTGAA
    ATTGTAGCACCGTTTGATGGTACAGTGAAAACAATCTTCCCTACGAAACATGCGATAGGA
    TTAGAATCTGAAAGTGGCGTCGAAGTACTTATTCATATTGGTATCGATACAGTGAAACTG
    AATGGTGAAGGATTCGAAAGTCTGATTAACGTTGATGAAAAAGTAACACAAGGTCAACCA
    TTAATGAAAGTGAATTTAGCATACTTGAAAGCACACGCACCAAGCATCGTTACACCAATG
    ATTATTACAAATCTTGAAAATAAAGAACTTGTCATTGAAGATGTACAAGATGCTGATCCA
    GGTAAGCTAATTATGACAGTCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2067

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2476 [new locus tag: SAUSA300_RS13740 ]
  • symbol: PtsG
  • description: phosphotransferase system, glucose-specific IIABC component
  • length: 688
  • theoretical pI: 6.13064
  • theoretical MW: 74415.8
  • GRAVY: 0.418895

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.69?  ExPASy
    Transferred entry: 2.7.1.191, 2.7.1.192, 2.7.1.193, 2.7.1.194, 2.7.1.195, 2.7.1.196, 2.7.1.197, 2.7.1.198, 2.7.1.199, 2.7.1.200, 2.7.1.201, 2.7.1.202, 2.7.1.203, 2.7.1.204, 2.7.1.205, 2.7.1.206, 2.7.1.207 and 2.7.1.208
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 849.4)
    and 13 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 451.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 380.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 303.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 277)
    Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 277)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 256.4)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 189.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 145.2)
    Signal transduction Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 145.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 70.9)
    Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 70.9)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 53.6)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.6)
  • TheSEED  :
    • PTS system, glucose-specific IIA component
    • PTS system, glucose-specific IIB component (EC 2.7.1.69)
    • PTS system, glucose-specific IIC component
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIA component
    and 2 more
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIB component (EC 2.7.1.69)
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIC component
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 320)
    and 3 more
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 168.3)
    no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 54.3)
    Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 18.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.412118
    • TAT(Tat/SPI): 0.014658
    • LIPO(Sec/SPII): 0.041457
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEIAPWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMNATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAWCYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLNKNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKDGVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITEPLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSHALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGGKENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHDMAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGKMMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEGFESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGKLIMTVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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