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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2487 [new locus tag: SAUSA300_RS13815 ]
  • pan locus tag?: SAUPAN006212000
  • symbol: feoB
  • pan gene symbol?: feoB
  • synonym:
  • product: ferrous iron transport protein B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2487 [new locus tag: SAUSA300_RS13815 ]
  • symbol: feoB
  • product: ferrous iron transport protein B
  • replicon: chromosome
  • strand: -
  • coordinates: 2687552..2689546
  • length: 1995
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAAATTATTGTATTTTAGGAAATCCTAATGTTGGTAAAACATCTTTGTTTAACGCT
    TTAACTGGTTCGTATGAATATATAGGCAACTGGAGTGGCGTAACGGTAGAGAAAAAAGTA
    GGGAAACTTAAAGAAAATGTAGGACAATTAATTGATTTACCAGGTACATATGATTTATCG
    CCGATTTCTAAAGATGAAACTGTAGTGACAGACTATTTATTAAACGATTCATTTTCAGGA
    ATCATTAACATTGTTGATGCGAGTCAACTAAAAAGAAATATGCAATTAACAGTACAATTG
    TTAGAACTTAATCAACCAATATACATCGGATTAAATATGATTGATGTAGCTACAAAGCGC
    GGGATAAAAATCGATTATCATAAATTGATGAAAAAGTTAAAAACACCTATTTTTCCAGTT
    GTGGCACGTACAGGCAAAGGAACAAAATATTTACTTGGCGAAATAAAGCATCTAGGGGAA
    GGGTATCAACCGCATTTTAAAATCAATTATGGTGAGAAGATTGAAGAGACAATTAAAAAT
    ATGTGTCAAATAATAATGACAGAGACATCTCATGATAAGTATCAAGCTAGGTTTATTGCG
    ATTCAATTCTTATTAAATAACATGCAAATTGCAAACGAACTTAATTCAGAGGTTGTAAAT
    AAATTGTCGAGTTTACGTGATCAAGTTGCTGAACAAGTAGGGGCTGTATCTGTTCGTCGA
    GAGATGGAGCGTATACGCAATCATTATATTGAGACGTTATTGCAAGACGTGGTAACGTAT
    CCAGATGAAGATAAGCAATATTTCAGCTCTAGAATAGACAAGATATTAACACATAAATAT
    ATAGGGATGCCTATCTTTCTAGCAATTATGTGGTTGATCTTTCAAACTACATTTACATGG
    ATTGGCACACCTTTGTCGGATCAACTCGATGCATTTATAGGTGGTACATTTACAGATAGT
    GTGAAGACGATTATGAATTATCTTGGTGTTATACCATTTTTACAAGATTTAATTACAGAT
    GGCATTATTGCTGGTGTAGGATCAGTATTAGTCTTTGTACCACAAATTGTTGTGCTCTTT
    TTCTTTATATCTTTATTAGAAGATTCGGGTTATATGGCACGTATTGCGGTTTTAATGGAT
    CGTATTATGGAATCGTTCGGTTTAAGCGGGAAGTCTTTTATACCAATGATTATCGGATTT
    GGTTGTAATGTACCGAGTATTATGGCTGCGCGCAGTATTGAAAATGAAAAAGAGCGTTTA
    ACAACGATATTAATTGCACCATTCATGTCATGCTCTGCTAGACTGCCAGTGTATGCGTTG
    TTCGTAGGTATCTTTTTCAAAGAGAATCAATCTTTAGTAGTATTAAGCCTGTATGTTTTA
    GGTATTATTATGGCATTTTTAGTCAGTACAGTACTTACTAAGACGATTTTAAAAAATGAT
    AATGCAATCTTTATTGTGGAATTGCCAACATACCGTGTGCCTTCCATTAAAACGTTGTGG
    CGTAGTACTTGGGAAAAGGCTAAGGGCTTTGTTCGTAAAGCAGGTACATTTATTTTTGGC
    GGCTCAGTAGTCATTTGGCTATTAAGTTATGTAGGACCACATGGTATCAATGTGAATATC
    AATCAAAGTTTTTTACATATGGTTGGTAGTTTCTTTGGTATGTTAGTGCAACCGCTCGGA
    TTTGGAACATGGCAAGCAGGTGCTACGTTAGTGCCAGGATTTTTAGCAAAAGAAGTTATC
    GTAAGTTCGATGGCGATTATCTATTCATCAGGTGATGCAGGCCTTGTTAATGTGATTCAA
    AATCAATTCACACCGCTATCTGCGTATGCATTTATGATATTTATACTATTGTATATTCCT
    TGTGTTTCTACAGTAGCAGCAATCCGTAAAGAGACTTATTCATGGAAATGGACGGCACTT
    GCAGTTGCCTATCCTCTAGTCACTGCATATGTCTTAACATTCATTTTTTATCAAGTCGGA
    CATTTATTTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1995

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2487 [new locus tag: SAUSA300_RS13815 ]
  • symbol: FeoB
  • description: ferrous iron transport protein B
  • length: 664
  • theoretical pI: 8.54816
  • theoretical MW: 74384.8
  • GRAVY: 0.367319

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 624.7)
    and 10 more
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 42.2)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 35.4)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 27.6)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 23.5)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 21.3)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 21.1)
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 17.8)
    Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 15.4)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 11.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 10.3)
  • TheSEED  :
    • Ferrous iron transport protein B
    CBSS-196620.1.peg.2477  Ferrous iron transport protein B
    and 1 more
    RCJ pfr  Ferrous iron transport protein B
  • PFAM:
    P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 191.9)
    and 6 more
    no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 152.3)
    FeoB_C; Ferrous iron transport protein B C terminus (PF07664; HMM-score: 67.9)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 56.1)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 21.7)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 13.2)
    Peptidase_CA (CL0125) Transglut_core2; Transglutaminase-like superfamily (PF13369; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.023195
    • TAT(Tat/SPI): 0.000284
    • LIPO(Sec/SPII): 0.003767
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLKENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLELNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGEGYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEVVNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKILTHKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFLQDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKSFIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQSLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAKGFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQAGATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVSTVAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

Can also transport cupric ions

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Valentyn Dzyhovskyi, Kamila Stokowa-Sołtys
    Divalent metal ion binding to Staphylococcus aureus FeoB transporter regions.
    J Inorg Biochem: 2023, 244;112203
    [PubMed:37018851] [WorldCat.org] [DOI] (I p)    Minhye Shin, Yerin Jin, Jinsub Park, Daye Mun, Soo Rin Kim, Shelley M Payne, Kyoung Heon Kim, Younghoon Kim
    Characterization of an Antibacterial Agent Targeting Ferrous Iron Transport Protein FeoB against Staphylococcus aureus and Gram-Positive Bacteria.
    ACS Chem Biol: 2021, 16(1);136-149
    [PubMed:33378170] [WorldCat.org] [DOI] (I p)
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