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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2490 [new locus tag: SAUSA300_RS13830 ]
  • pan locus tag?: SAUPAN006215000
  • symbol: SAUSA300_2490
  • pan gene symbol?: farR
  • synonym:
  • product: TetR family regulatory protein

farR: unsaturated fatty acid resistance transcriptional activator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2490 [new locus tag: SAUSA300_RS13830 ]
  • symbol: SAUSA300_2490
  • product: TetR family regulatory protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2692635..2693183
  • length: 549
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAAGAGACTGATTTACGAGTTATAAAGACAAAAAAAGCATTGTCGAGTAGCTTGCTA
    CAATTGTTAGAACAGCAATTATTCCAAACGATTACTGTCAATCAAATTTGCGACAACGCA
    CTCGTACACCGTACAACATTTTATAAACATTTTTATGATAAATATGATCTTCTAGAGTAC
    TTGTTCAATCAATTGACTAAAGACTACTTTGCTAGAGATATCAGTGACCGTCTTAATCAT
    CCATTCCAAACGATGAGTGATACGATTAATAATAAAGAGGATTTGAGAGAAATCGCAGAA
    TTCCAAGAAGAAGACGCTGAATTTAATAAAGTATTAAAAAATGTCTGCATTAAAATTATG
    CATAACGATATCAAAAATAATAGAGACCGTATCGATATTGACAGCGACATCCCAGATAAT
    CTCATATTTTATATTTATGACTCGTTGATTGAAGGTTTTATACATTGGATAAAAGATGAA
    AAAATTGATTGGCCTGGCGAAGATATTGATAACATTTTCCATAGATTAATCAATATTAAG
    ATTAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    549

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2490 [new locus tag: SAUSA300_RS13830 ]
  • symbol: SAUSA300_2490
  • description: TetR family regulatory protein
  • length: 182
  • theoretical pI: 4.96153
  • theoretical MW: 21878.7
  • GRAVY: -0.522527

⊟Function[edit | edit source]

  • TIGRFAM:
    probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 50.1)
    and 2 more
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 20.9)
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 13.7)
  • TheSEED  :
    • Transcriptional regulator, AcrR family
    CBSS-196620.1.peg.2477  TetR family regulatory protein
  • PFAM:
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 22.7)
    and 2 more
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 17.2)
    HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021872
    • TAT(Tat/SPI): 0.000407
    • LIPO(Sec/SPII): 0.003798
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHRTTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQEEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDEKIDWPGEDIDNIFHRLINIKIK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Heba Alnaseri, Robert C Kuiack, Katherine A Ferguson, James E T Schneider, David E Heinrichs, Martin J McGavin
    DNA Binding and Sensor Specificity of FarR, a Novel TetR Family Regulator Required for Induction of the Fatty Acid Efflux Pump FarE in Staphylococcus aureus.
    J Bacteriol: 2019, 201(3);
    [PubMed:30455282] [WorldCat.org] [DOI] (I e)
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