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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2500 [new locus tag: SAUSA300_RS13885 ]
  • pan locus tag?: SAUPAN006227000
  • symbol: SAUSA300_2500
  • pan gene symbol?: crtQ
  • synonym:
  • product: glycosyl transferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2500 [new locus tag: SAUSA300_RS13885 ]
  • symbol: SAUSA300_2500
  • product: glycosyl transferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2704559..2705686
  • length: 1128
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAATGGTTATCACGAATATTAACAGTAATAGTGACCATGTCTATGGCGTGTGGTGCA
    TTGATATTTAATCGTAGACATCAGCTAAAGGCGAAAACGCTGAACTTCAATCATAAAGCA
    TTAACAATTATTATTCCGGCTAGAAACGAAGAAAAAAGAATAGGTCATTTACTACATTCG
    ATAATACAACAGCAAGTTCCAGTAGATGTCATTGTTATGAATGACGGATCGACAGATGAA
    ACAGCACGTGTAGCACGTTCATATGGTGCGACTGTAGTAGATGTTGTTGATGATACTGAC
    GGCAAATGGTATGGGAAATCACATGCTTGTTATCAAGGTGTGACGCATGCATGTACGAAT
    CGCATTGCCTTTGTAGATGCTGATGTAACTTTCTTAAGGAAAGATGCTGTTGAAACGTTG
    ATTAATCAGTATCAATTACAAGGTGAAAAAGGATTGTTAAGCGTACAGCCTTATCATATA
    ACAAAGCGTTTCTACGAAGGGTTTTCAGCGATATTTAATTTAATGACAGTCGTTGGTATG
    AATGTATTTTCTACCTTAGACGACGGTCGGACTAACCAGCATGCATTTGGACCGGTGACA
    TTAACAAATAAAGAAGATTATTATGCAACTGGAGGTCATAAAAGTGCAAACCGTCATATT
    ATTGAAGGATTTGCTTTAGGAAGTGCATATACTTCACAATCATTGCCCGTAACAGTTTAT
    GAAGGGTTTCCATTTGTTGCATTTCGCATGTATCAAGAAGGATTTCAGTCATTACAAGAA
    GGATGGACAAAGCATTTGTCAACTGGGGCAGGTGGCACAAAGCCTAAGATCATGACAGCA
    ATTGTGTTGTGGTTGTTTGGTTCTATAGCGAGTATTTTAGGGCTATGTCTTAGTTTAAAA
    TATCGCCAAATGTCTGTAAGAAAAATGGTAGCACTTTACTTGAGCTATACTACACAATTT
    ATTTATCTGCATCGAAGGGTCGGCCAATTTTCTAATTTATTAATGGTATGTCATCCATTG
    TTATTTATGTTTTTTACTAAAATTTTCATCCAATCTTGGAAACAAACGCATCGTTATGGT
    GTAGTTGAATGGAAAGGTCGTCAATATTCTATATCTAAAGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1128

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2500 [new locus tag: SAUSA300_RS13885 ]
  • symbol: SAUSA300_2500
  • description: glycosyl transferase
  • length: 375
  • theoretical pI: 9.84953
  • theoretical MW: 42543.9
  • GRAVY: -0.044

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.-?  ExPASy
  • TIGRFAM:
    hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 60.4)
    and 8 more
    Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 47.4)
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 45.9)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 42.4)
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 42.2)
    putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 29.9)
    hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 15.1)
    Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 13.1)
    glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 12.8)
  • TheSEED  :
    • 4,4'-diaponeurosporenoate glycosyltransferase (EC 2.4.1.-)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  4,4'-diaponeurosporenoate glycosyltransferase (EC 2.4.1.-)
  • PFAM:
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 61.2)
    and 6 more
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 44.5)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 37.3)
    NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 20.2)
    Glyco_transf_21; Glycosyl transferase family 21 (PF13506; HMM-score: 17.7)
    Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 16.9)
    2heme_cytochrom (CL0328) Ferric_reduct; Ferric reductase like transmembrane component (PF01794; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.019225
    • TAT(Tat/SPI): 0.003522
    • LIPO(Sec/SPII): 0.936878
    • Cleavage Site: CS pos: 17-18. SMA-CG. Pr: 0.9397
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTIIIPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGKWYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHITKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANRHIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPKIMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLLMVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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