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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2578 [new locus tag: SACOL_RS13505 ]
  • pan locus tag?: SAUPAN006227000
  • symbol: SACOL2578
  • pan gene symbol?: crtQ
  • synonym:
  • product: glycosyl transferase, group 2 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2578 [new locus tag: SACOL_RS13505 ]
  • symbol: SACOL2578
  • product: glycosyl transferase, group 2 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2641254..2642390
  • length: 1137
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    GTGATATCTATGAAATGGTTATCACGAATATTAACAGTAATAGTGACCATGTCTATGGCG
    TGTGGTGCATTGATATTTAATCGTAGACATCAGCTAAAGGCGAAAACGCTGAACTTCAAT
    CATAAAGCATTAACAATTATTATTCCGGCTAGAAACGAAGAAAAAAGAATAGGTCATTTA
    CTACATTCGATAATACAACAGCAAGTTCCAGTAGATGTCATTGTTATGAATGACGGATCG
    ACAGATGAAACAGCACGTGTAGCACGTTCATATGGTGCGACTGTAGTAGATGTTGTTGAT
    GATACTGACGGCAAATGGTATGGGAAATCACATGCTTGTTATCAAGGTGTGACGCATGCA
    TGTACGAATCGCATTGCCTTTGTAGATGCTGATGTAACTTTCTTAAGGAAAGATGCTGTT
    GAAACGTTGATTAATCAGTATCAATTACAAGGTGAAAAAGGATTGTTAAGCGTACAGCCT
    TATCATATAACAAAGCGTTTCTACGAAGGGTTTTCAGCGATATTTAATTTAATGACAGTC
    GTTGGTATGAATGTATTTTCTACCTTAGACGACGGTCGGACTAACCAGCATGCATTTGGA
    CCGGTGACATTAACAAATAAAGAAGATTATTATGCAACTGGAGGTCATAAAAGTGCAAAC
    CGTCATATTATTGAAGGATTTGCTTTAGGAAGTGCATATACTTCACAATCATTGCCCGTA
    ACAGTTTATGAAGGGTTTCCATTTGTTGCATTTCGCATGTATCAAGAAGGATTTCAGTCA
    TTACAAGAAGGATGGACAAAGCATTTGTCAACTGGGGCAGGTGGCACAAAGCCTAAGATC
    ATGACAGCAATTGTGTTGTGGTTGTTTGGTTCTATAGCGAGTATTTTAGGGCTATGTCTT
    AGTTTAAAATATCGCCAAATGTCTGTAAGAAAAATGGTAGCACTTTACTTGAGCTATACT
    ACACAATTTATTTATCTGCATCGAAGGGTCGGCCAATTTTCTAATTTATTAATGGTATGT
    CATCCATTGTTATTTATGTTTTTTACTAAAATTTTCATCCAATCTTGGAAACAAACGCAT
    CGTTATGGTGTAGTTGAATGGAAAGGTCGTCAATATTCTATATCTAAAGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1137

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2578 [new locus tag: SACOL_RS13505 ]
  • symbol: SACOL2578
  • description: glycosyl transferase, group 2 family protein
  • length: 378
  • theoretical pI: 9.84953
  • theoretical MW: 42875.4
  • GRAVY: -0.028836

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.-?  ExPASy
  • TIGRFAM:
    hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 59.9)
    and 8 more
    Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 47.4)
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 46)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 42.4)
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 42.2)
    putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 30.3)
    hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 15.5)
    Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 13.1)
    glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 12.8)
  • TheSEED  :
    • 4,4'-diaponeurosporenoate glycosyltransferase (EC 2.4.1.-)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  4,4'-diaponeurosporenoate glycosyltransferase (EC 2.4.1.-)
  • PFAM:
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 61.2)
    and 6 more
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 44.5)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 37.2)
    NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 20.2)
    Glyco_transf_21; Glycosyl transferase family 21 (PF13506; HMM-score: 17.7)
    Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 16.9)
    2heme_cytochrom (CL0328) Ferric_reduct; Ferric reductase like transmembrane component (PF01794; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.012202
    • TAT(Tat/SPI): 0.006547
    • LIPO(Sec/SPII): 0.941396
    • Cleavage Site: CS pos: 20-21. SMA-CG. Pr: 0.9437
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISMKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTIIIPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGKWYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHITKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANRHIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPKIMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLLMVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [2] [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  3. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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