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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2580 [new locus tag: SACOL_RS13515 ]
  • pan locus tag?: SAUPAN006229000
  • symbol: SACOL2580
  • pan gene symbol?: crtO
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2580 [new locus tag: SACOL_RS13515 ]
  • symbol: SACOL2580
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2643873..2644361
  • length: 489
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAAAAATATATTAAAACAGCATTTTTTTGTAGTATGTATTGGTTAATTGTTCAACTA
    AATATAGCAAATTTAGGTACAAGAATTCCTGATAAGTATTTTCGTCAGAAGTACATAATA
    TTTAAATCATTTAACTTTGAGAAGCATGGAAAATTTTGGAACAAATGGTTTTACGTAAGA
    AAATGGAAACATAAGATTTTAGATGGTCATCAGCTTAATCAAAATATATATGATCAGCGT
    CATTTAATGACAATCAATACTGATGAAATTGAAAAAATGATTATAGAGACAAAGAGGGCA
    GAGTTGATTCATTGGATATCGATACTTCCAGTCATCATATTCAATAAAGGCCCTCGTTTA
    GTAAAGTATATAAATATTTTCTATGCAATGATAGCTAATGTTCCAATCATTATTGTGCAA
    CGCTATAATCGACCGAGATTAACGCAGTTACTACGCATATTAAAACGAAGAGGTGAACGT
    CATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    489

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2580 [new locus tag: SACOL_RS13515 ]
  • symbol: SACOL2580
  • description: hypothetical protein
  • length: 162
  • theoretical pI: 10.8573
  • theoretical MW: 19958.6
  • GRAVY: -0.288272

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • Glycosyl-4,4'-diaponeurosporenoate acyltransferase precursor (EC 2.3.1.-)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  Glycosyl-4,4'-diaponeurosporenoate acyltransferase precursor (EC 2.3.1.-)
  • PFAM:
    no clan defined Peptidase_S80; Bacteriophage T4-like capsid assembly protein (Gp20) (PF07230; HMM-score: 15.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.5
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.224674
    • TAT(Tat/SPI): 0.001771
    • LIPO(Sec/SPII): 0.081329
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSFNFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAELIHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGERHD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Signal peptide containing [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [2] [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  3. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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