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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2528 [new locus tag: SAUSA300_RS14025 ]
  • pan locus tag?: SAUPAN006272000
  • symbol: SAUSA300_2528
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2528 [new locus tag: SAUSA300_RS14025 ]
  • symbol: SAUSA300_2528
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2729533..2730255
  • length: 723
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATTAATGCTGAACCTATTATCAGTAAAATGAAAAATCAAAAAATTAATTATGACAAG
    GTGTTGAAGAAATTAATCGGGCAGTGGGAAAGAGAAGCAATCAGACCTAAAATCTTATTG
    CATAGTTGTTGTGCACCTTGTAGTACATATACATTAGAGTTTTTAACACAATATGCAGAC
    ATTGCAATTTATTTCGCGAATTCAAATATTCATCCGAAAAATGAGTACTTACGACGTGCT
    AAAGTGCAAGAACAATTTGTGGAAGATTTTAATCGCAAGACTGGTGCGAATGTGAAGTAT
    ATTGAAGCACCTTATGAACCGCATAAGTTTGTGAAAATGGTGAAGGATAAAGAATTAGCT
    GATGAAAAAGAAGGCGGCTTACGTTGTACCGCTTGTTTTGAAATGCGTTTGGATATTGTA
    GCGAAAGCAGCTGTAGAACATGGCTATGATTATTTTGGCAGTGCAATCACATTATCACCT
    AAAAAGAACGCACAATTAATCAATGAACTTGGTATGGATGTCCAAAAAATATACGATGTG
    AACTATTTGCCAAGTGATTTTAAGAAAAGTAAAGGTTATGAGCGTTCCATAGAAATGTGT
    AATGACTATAATATCTTTAGGCAGTGTTATTGTGGATGTGTCTTTGCAGCGATGCAACAA
    GGTATTGATTTTAAAACAGTTAATAAAGAAGCGAAAGCATTTTTAGAACAATATCCAGAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    723

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2528 [new locus tag: SAUSA300_RS14025 ]
  • symbol: SAUSA300_2528
  • description: hypothetical protein
  • length: 240
  • theoretical pI: 8.11805
  • theoretical MW: 27788.9
  • GRAVY: -0.46375

⊟Function[edit | edit source]

  • reaction:
    EC 1.17.99.6?  ExPASy
    Epoxyqueuosine reductase Queuosine34 in tRNA + acceptor + H2O = epoxyqueuosine34 in tRNA + reduced acceptor
  • TIGRFAM:
  • TheSEED  :
    • Epoxyqueuosine reductase (EC 1.17.99.6) QueH
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  Epoxyqueuosine reductase (EC 1.17.99.6) QueH
  • PFAM:
    no clan defined DUF208; Uncharacterized BCR, COG1636 (PF02677; HMM-score: 205.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.040551
    • TAT(Tat/SPI): 0.000493
    • LIPO(Sec/SPII): 0.017156
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCCAPCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIEAPYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSPKKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAMQQGIDFKTVNKEAKAFLEQYPD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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