PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2598 [new locus tag: SAUSA300_RS14445 ]
• pan locus tag^?: SAUPAN006409000
• symbol: cap1A
• pan gene symbol^?: cap1A
• synonym:
• product: capsular polysaccharide biosynthesis protein Cap1A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2598 [new locus tag: SAUSA300_RS14445 ]
• symbol: cap1A
• product: capsular polysaccharide biosynthesis protein Cap1A
• replicon: chromosome
• strand: -
• coordinates: 2824377..2825039
• length: 663
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915133 NCBI
• RefSeq: YP_495232 NCBI
• BioCyc: see SAUSA300_RS14445
• MicrobesOnline: 1294113 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAGAAAAGTTTGATTTAGTAAAACTATTAAATATTCTAAAGAAGAATATTAAATTA
  TTGCTTATTTTACCGGCAATATGTCTTGTAGTAAGTGCGGCATTAACCTTTTTTGTTATG
  CCTGACAAATATACTGCTTCTACTCAAATATTAGTGAACATGAAAAAGTCCTCAAGTGAT
  TTAGCTTTCCAAAATGTTCAAAGTAGTTTGCAGTCTGTTAATACATATACGGAAATTATC
  AAAAGTCCTAGAATTCTAGATAAAGTATCTAGAGAATTTGATGGTCAGTATTCAACAGCT
  GAGTTGAATTCATTTTTAAAAGTAACCAATCAAACAAACTCTCAAATTATTACTGTATCA
  GTTACCACTGGGAATAAATCTGAATCTGACAAAATTGTTAATAAAATATCTAAAGTTTTT
  GCTCATGACATGCCTAAAATTATGAGTGTTGATAATGTTACGATACTTTCCTCAGCACAT
  GATAATGCTGTAAAAGTATCTCCAATAGTATCTGTCAACTTAGTTATCAGCATTATTGTT
  GGTATTGTTTTAGCAATATTGATTATTTTCTTAAAAGAATTATTAGATAAGCGTATTAAG
  ACAGAAGAAGATGTTGAATCGCAACTAGGATTACCTATTTTAGGTTCAATACAAAAATTT
  TAA
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  663
  

⊟Protein

⊟General

• locus tag: SAUSA300_2598 [new locus tag: SAUSA300_RS14445 ]
• symbol: Cap1A
• description: capsular polysaccharide biosynthesis protein Cap1A
• length: 220
• theoretical pI: 9.8166
• theoretical MW: 24339.5
• GRAVY: 0.299091

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polysaccharide export protein, MPA1 family (TIGR01006; HMM-score: 240.8)
  and 4 more
  chain length determinant protein EpsF (TIGR03017; HMM-score: 55.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 41.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide chain length determinant protein, PEP-CTERM locus subfamily (TIGR03007; HMM-score: 29.4)
  NDMA-dependent methanol dehydrogenase (TIGR04266; EC 1.1.99.37; HMM-score: 15.4)
• TheSEED  :
  • Tyrosine-protein kinase transmembrane modulator EpsC
  Cell Wall and Capsule Capsular and extracellular polysacchrides Exopolysaccharide Biosynthesis  Tyrosine-protein kinase transmembrane modulator EpsC
  and 1 more
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Tyrosine-protein kinase transmembrane modulator EpsC
• PFAM:
  no clan defined Wzz; Chain length determinant protein (PF02706; HMM-score: 82.6)
  and 7 more
  GNVR; G-rich domain on putative tyrosine kinase (PF13807; HMM-score: 20.1)
  Amnionless; Amnionless (PF14828; HMM-score: 13.9)
  DUF2975; Protein of unknown function (DUF2975) (PF11188; HMM-score: 13.7)
  CD34_antigen; CD34/Podocalyxin family (PF06365; HMM-score: 12.6)
  VSA (CL0656) RIFIN; Rifin (PF02009; HMM-score: 11.1)
  FUSC (CL0307) FUSC; Fusaric acid resistance protein family (PF04632; HMM-score: 8.8)
  no clan defined DUF5362; Family of unknown function (DUF5362) (PF17319; HMM-score: 8.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.154438
  • TAT(Tat/SPI): 0.003094
  • LIPO(Sec/SPII): 0.028865
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87160242 NCBI
• RefSeq: YP_495232 NCBI
• UniProt: A0A0H2XE46 UniProt

⊟Protein sequence

• MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKYTASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAELNSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAHDNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQKF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cap1C < cap1B < cap1A

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications