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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS00130 [old locus tag: SAUSA300_0025 ]
  • pan locus tag?: SAUPAN000037000
  • symbol: SAUSA300_RS00130
  • pan gene symbol?: adsA
  • synonym:
  • product: multifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'-nucleotidase/3'-nucleotidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS00130 [old locus tag: SAUSA300_0025 ]
  • symbol: SAUSA300_RS00130
  • product: multifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'-nucleotidase/3'-nucleotidase
  • replicon: chromosome
  • strand: +
  • coordinates: 31026..33344
  • length: 2319
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAAGCTTTATTACTTAAAACAAGTGTATGGCTCGTTTTGCTTTTTAGTGTAATGGGA
    TTATGGCAAGTCTCGAACGCGGCTGAGCAGCATACACCAATGAAAGCACATGCAGTAACA
    ACGATAGACAAAGCAACAACAGATAAGCAACAAGTACCGCCAACAAAGGAAGCGGCTCAT
    CATTCTGGCAAAGAAGCGGCAACCAACGTATCAGCATCAGCGCAGGGAACAGCTGATGAT
    ACAAACAGCAAAGTAACATCCAACGCACCATCTAACAAACCATCTACAGTAGTTTCAACA
    AAAGTAAACGAAACACGCGACGTAGATACACAACAAGCCTCAACACAAAAACCAACTCAC
    ACAGCAACGTTCAAATTATCAAATGCTAAAACAGCATCACTTTCACCACGAATGTTTGCT
    GCTAATGCACCACAAACAACAACACATAAAATATTACATACAAATGATATCCATGGCCGA
    CTAGCCGAAGAAAAAGGGCGTGTCATCGGTATGGCTAAATTAAAAACAGTAAAAGAACAA
    GAAAAGCCTGATTTAATGTTAGACGCAGGAGACGCCTTCCAAGGTTTACCACTTTCAAAC
    CAGTCTAAAGGTGAAGAAATGGCTAAAGCAATGAATGCAGTAGGTTATGATGCTATGGCA
    GTCGGTAACCATGAATTTGACTTTGGATACGATCAGTTGAAAAAGTTAGAGGGTATGTTA
    GACTTCCCGATGCTAAGTACTAACGTTTATAAAGATGGAAAACGCGCGTTTAAGCCTTCA
    ACGATTGTAACAAAAAATGGTATTCGTTATGGAATTATTGGTGTAACGACACCAGAAACA
    AAGACGAAAACAAGACCTGAAGGCATTAAAGGCGTTGAATTTAGAGATCCATTACAAAGT
    GTGACAGCGGAAATGATGCGTATTTATAAAGACGTAGATACATTTGTTGTTATATCACAT
    TTAGGAATTGATCCTTCAACACAAGAAACATGGCGTGGTGATTACTTAGTGAAACAATTA
    AGTCAAAATCCACAATTGAAGAAACGTATTACAGTTATTGATGGTCATTCACATACAGTA
    CTTCAAAATGGTCAAATTTATAACAATGATGCATTGGCACAAACAGGTACAGCACTTGCG
    AATATCGGTAAGATTACATTTAATTATCGCAATGGAGAGGTATCGAATATTAAACCGTCA
    TTGATTAATGTTAAAGACGTTGAAAATGTAACACCGAACAAAGCATTAGCTGAACAAATT
    AATCAAGCTGATCAAACATTTAGAGCACAAACTGCAGAGGTAATTATTCCAAACAATACC
    ATTGATTTCAAAGGAGAAAGAGATGACGTTAGAACGCGTGAAACAAATTTAGGAAACGCG
    ATTGCAGATGCTATGGAAGCGTATGGCGTTAAGAATTTCTCTAAAAAGACTGACTTTGCC
    GTGACAAATGGTGGAGGTATTCGTGCCTCTATCGCAAAAGGTAAGGTGACACGCTATGAT
    TTAATCTCAGTATTACCATTTGGAAATACGATTGCGCAAATTGATGTAAAAGGTTCAGAC
    GTCTGGACGGCTTTCGAACATAGTTTAGGCGCACCAACAACACAAAAGGACGGTAAGACA
    GTGTTAACAGCGAATGGCGGTTTACTACATATCTCTGATTCAATCCGTGTTTACTATGAT
    ATAAATAAACCGTCTGGCAAACGAATTAATGCTATTCAAATTTTAAATAAAGAGACAGGT
    AAGTTTGAAAATATTGATTTAAAACGTGTATATCACGTAACGATGAATGACTTCACAGCA
    TCAGGTGGCGACGGATATAGTATGTTCGGTGGTCCTAGAGAAGAAGGTATTTCATTAGAT
    CAAGTACTAGCAAGTTATTTAAAAACAGCTAACTTAGCTAAGTATGATACGACAGAACCA
    CAACGTATGTTATTAGGTAAACCAGCAGTAAGTGAACAACCAGCTAAAGGACAACAAGGT
    AGCAAAGGTAGTAAGTCTGGTAAAGATACACAACCAATTGGTGACGACAAAGTGATGGAT
    CCAGCGAAAAAACCAGCTCCAGGTAAAGTTGTATTGTTGCTAGCGCATAGAGGAACTGTT
    AGTAGCGGTACAGAAGGTTCTGGTCGCACAATAGAAGGAGCTACTGTATCAAGCAAGAGT
    GGGAAACAATTGGCTAGAATGTCAGTGCCTAAAGGTAGCGCGCATGAGAAACAGTTACCA
    AAAACTGGAACTAATCAAAGTTCAAGCCCAGAAGCGATGTTTGTATTATTAGCAGGTATA
    GGTTTAATCGCGACTGTACGACGTAGAAAAGCTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2319

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS00130 [old locus tag: SAUSA300_0025 ]
  • symbol: SAUSA300_RS00130
  • description: multifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'-nucleotidase/3'-nucleotidase
  • length: 772
  • theoretical pI: 10.0493
  • theoretical MW: 83424
  • GRAVY: -0.480959

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 134.4)
    Metabolism Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 134.4)
    and 6 more
    thiosulfohydrolase SoxB (TIGR04486; HMM-score: 91.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other 2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 52.7)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair exonuclease SbcCD, D subunit (TIGR00619; HMM-score: 13.1)
    aspartate-alanine antiporter (TIGR03802; HMM-score: 10.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking membrane protein insertase, YidC/Oxa1 family, N-terminal domain (TIGR03593; HMM-score: 8)
  • TheSEED: see SAUSA300_0025
  • PFAM:
    no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 141.4)
    and 3 more
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 43.6)
    no clan defined IES5; Ino80 complex subunit 5 (PF17335; HMM-score: 14.2)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.991407
    • TAT(Tat/SPI): 0.001132
    • LIPO(Sec/SPII): 0.004121
    • Cleavage Site: CS pos: 27-28. SNA-AE. Pr: 0.8858
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDKATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKVNETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGRLAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDAMAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVTTPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGDYLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNGEVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDVRTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFGNTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSGKRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLASYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPAKKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLPKTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Vilasack Thammavongsa, Justin W Kern, Dominique M Missiakas, Olaf Schneewind
Staphylococcus aureus synthesizes adenosine to escape host immune responses.
J Exp Med: 2009, 206(11);2417-27
[PubMed:19808256] [WorldCat.org] [DOI] (I p)Vilasack Thammavongsa, Olaf Schneewind, Dominique M Missiakas
Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA).
BMC Biochem: 2011, 12;56
[PubMed:22035583] [WorldCat.org] [DOI] (I e)Hwan Keun Kim, Vilasack Thammavongsa, Olaf Schneewind, Dominique Missiakas
Recurrent infections and immune evasion strategies of Staphylococcus aureus.
Curr Opin Microbiol: 2012, 15(1);92-9
[PubMed:22088393] [WorldCat.org] [DOI] (I p)

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