PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00140 [old locus tag: SAUSA300_0027 ]
• pan locus tag^?: SAUPAN000041000
• symbol: SAUSA300_RS00140
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00140 [old locus tag: SAUSA300_0027 ]
• symbol: SAUSA300_RS00140
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 34513..35769
• length: 1257
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (34513..35769) NCBI
• BioCyc: SAUSA300_RS00140 BioCyc
• MicrobesOnline: see SAUSA300_0027

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATAGACAACTTTATGCAGGTCCTTAAATTAATTAAAGAGAAACGTACCAATAATGTA
  GTTAAAAAATCTGATTGGGATAAAGGTGATCTATATAAAACTTTAGTCCATGATAAGTTA
  CCCAAGCAGTTAAAAGTGCATATAAAAGAAGATAAATATTCAGTTGTAGGGAAGGTTGCT
  ACTGGGAACTATAGTAAAGTTCCTTGGATTTCAATATATGATGAGAATATAACAAAAGAA
  ACAAAGGATGGATATTATTTGGTATATCTTTTTCATCCGGAAGGAGAAGGCATATACTTA
  TCTTTGAATCAAGGATGGTCAAAGATAAGTGATATGTTTCCGCGGGATAAAAATGCTGCA
  AAACAAAGAGCATTAACTTTATCTTCCGAACTCAATAAATATATTACATCAAATGAATTT
  AATACTGGAAGATTTTATTACGCAGAAAATAAAGATTCATCTTATGATTTAAAAAATGAT
  TATCCATCAGGATATTCTCATGGATCAATAAGATTCAAATATTATGATTTGAATGAAGGA
  TTCACAGAAGAAGATATGCTAGAGGATTTAAAGAAATTTTTAGAACTATTTAATGAATTA
  GCTTCAAAAGTTACAAAAACATCCTATGATAGCTTGGTCAATAGCATAGACGAAATACAG
  GAAGACAGCGAAATTGAAGAAATTAGAACAGCACAAAAAGATAAGACACTCAAGGAAGTG
  GAAGCACCTAAAGGAATAATTCCAAAATATAAAAAAGGTGTATCAAAGACTACTAAAAAT
  GATTCAGAAATTGAAAAATCAAATAAAGAGAATAAATTAACCGGTAAAGTTGGAGAAAAA
  TTAGCGCTAAATTACTTTAATGAGCTAATTGATAATAAAATAGACGAAGATAAGAAAGAA
  CAGTTTAGGAATATTTTAAATGATAATCCAGGCTCTCAACACGGTCATGGCTATGATTTA
  GTAGCTTTTGATCCAACAAATACAGATAAAGCTGTAGAAAAATTTATTGAAATTAAAACA
  TCTACATCTTCTAGTATTGAGGAACCATTTTTTATGTCGCTAAATGAAATGTTTGCTATG
  AAAGAATATAAGCAGAAATATTTAATATTAAGAATATTTAATGTTTCCGGTAAAGAACCA
  CAATTTTATTTTATAGATCCATATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTC
  ATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGA
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  1257
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00140 [old locus tag: SAUSA300_0027 ]
• symbol: SAUSA300_RS00140
• description: hypothetical protein
• length: 418
• theoretical pI: 5.32851
• theoretical MW: 48642.4
• GRAVY: -0.775359

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_0027
• PFAM:
  no clan defined MrcB_N; MrcB-like, N-terminal domain (PF12102; HMM-score: 166)
  and 6 more
  PDDEXK (CL0236) NOV_C; Protein NO VEIN, C-terminal (PF13020; HMM-score: 63.9)
  Hybrid (CL0105) RPOC_hybrid; DNA-directed RNA polymerase subunit beta', hybrid domain (PF21668; HMM-score: 14.2)
  no clan defined DUF6985; Domain of unknown function (DUF6985) (PF22481; HMM-score: 12.2)
  SHCBP_N; SHCBP N-terminal domain (PF23762; HMM-score: 11.2)
  OVT1; Major antigen, helical domain (PF24423; HMM-score: 10.3)
  G1_gp67_C; Phage G1 gp67, C-terminal domain (PF20881; HMM-score: 7.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7908
  • Cytoplasmic Membrane Score: 0.0104
  • Cell wall & surface Score: 0.0035
  • Extracellular Score: 0.1953
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012861
  • TAT(Tat/SPI): 0.000426
  • LIPO(Sec/SPII): 0.00161
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446488816 NCBI
• RefSeq: WP_000566670 NCBI
• UniProt: see SAUSA300_0027

⊟Protein sequence

• MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQLKVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSLNQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKNDYPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDEIQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGKVGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEKFIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYSEFKDVDDLIDKVFNVEAIQYKVFGEK

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications