PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00560 [old locus tag: SAUSA300_0108 ]
• pan locus tag^?: SAUPAN000902000
• symbol: SAUSA300_RS00560
• pan gene symbol^?: —
• synonym:
• product: oleate hydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00560 [old locus tag: SAUSA300_0108 ]
• symbol: SAUSA300_RS00560
• product: oleate hydratase
• replicon: chromosome
• strand: -
• coordinates: 120990..122765
• length: 1776
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (120990..122765) NCBI
• BioCyc: SAUSA300_RS00560 BioCyc
• MicrobesOnline: see SAUSA300_0108

⊟Phenotype

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⊟DNA sequence

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  ATGTATTACAGTTATGGAAATTATGAAGCATTTGCGCGCCCTAAAAAACCTGAAAATGTA
  GAAAACAAATCCGCTTACTTAATCGGATCTGGTCTAGCTTCACTTGCTGCAGCTTGTTTT
  TTAATAAGGGATGGTCAAATGGAAGGTTCGAAGATTCATATTTTAGAAGAGTTACCTAAA
  GCAGGTGGTAGTCTTGATGGTGAAAATATGCCTTTAAAAGGCTATGTTGTCCGCGGTGGT
  CGTGAAATGGAGAACCACTTTGAATGTTTGTGGGACTTATTCAGATCTATCCCTTCATTA
  GAAATCGATAACGCGTCTGTATTAGATGAGTTCTATTGGCTAAACAAAGAAGACCCTAAC
  TATTCTCGCTGTCGTGTTATTGAGAAACAGGGTCAACGTTTAGTCACAGACGGAGACTTC
  ACTTTGACTAAAACGGCGATTAAAGAAATTTTAGATTTATGCTTAACGAATGAAGAAGAT
  TTAGATGATGTCAAAATAACAGATGTATTTTCCGATGACTTCTTTAATTCAAACTTTTGG
  ATTTACTGGAAAACGATGTTTGCATTTGAACCGTGGCATTCTGCAATGGAAATGCGTCGC
  TATCTAATGCGATTCGTTCATCATATTAGTGGTCTCGCAGACTTTTCAGCTTTAAAATTC
  ACTAAATATAATCAATATGAATCTTTAGTATTACCTATGGTTGAATATTTAAAATCGCAT
  GGGGTTCAATTTGAATACGATGTAAAAGTCGAAGATATTAAAATAGATGTTACGACAAGT
  CAAAAAATTGCCCGAGAAATATTAATTGACCGTAATGGTAATGCAGAATCTATTAAACTG
  ACTATAAACGATCTTGTCTTTGTGACAAACGGTAGTATTACAGAAAGCTCTACTTATGGT
  GATAATGATACACCAGCGCCACCAACTGACGAATTAGGTGGTAGTTGGACACTATGGAAA
  AATTTAGCGCGACAAAGTCCTGAATTTGGTAATCCTGATAAGTTTTGCCAAAATATTCCT
  AAAAAAAGTTGGTTTGTTTCAGCAACTTCTACAACAAACAATAAAGAGATTATCGATACA
  ATAGAAAGTATTTGTAAACGTGACCCACTTGCAGGCAAAACAGTTACAGGCGGTATTATT
  ACAATCAATGATTCTGCATGGCAAATGAGTTTTACAATCAATCGTCAGCAACAGTTTAAA
  GACCAACCTGAAAATGAAATATCTACATGGATTTATGCCTTATATTCAGATGTAAACGGC
  GATTATATTAAAAAGCCAATTACAGAATGTAGTGGTAATGAAATATGCCAAGAATGGCTG
  TATCACTTAGGTGTATCAACTGACAAAATTGAAGACTTAGCAAAACATGCATCTAATACG
  ATTCCTGTTTATATGCCATATATCACATCTTATTTCATGACGCGTGCTATCGGCGACAGA
  CCTTTAGTCGTCCCGCATCAATCTCAGAACTTAGCATTTATTGGTAACTTTGCAGAAACA
  GAGCGAGACACTGTATTTACAACAGAATATTCGGTTCGTACTGCCATGGAAGCTGTTTAT
  CAATTACTAAATATAGATCGTGGTATTCCAGAAGTCATCAATAGTCCATTTGATCTTCGC
  GTCTTAATGGATGCCATATACGAACTGAATGACCACCAAGATTTGCGTGAGATTACTAAA
  GATTCGAAAATGCAAAAACTCGCATTAGCAGGATTCCTTAAAAAGATAAAAGGTACGTAC
  ATTGAGTCATTATTAAAAGAACACAAATTGTTATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS00560 [old locus tag: SAUSA300_0108 ]
• symbol: SAUSA300_RS00560
• description: oleate hydratase
• length: 591
• theoretical pI: 4.75677
• theoretical MW: 67648.2
• GRAVY: -0.371235

⊟Function

• TIGRFAM:
  9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15.7)
  squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 14.8)
• TheSEED: see SAUSA300_0108
• PFAM:
  NADP_Rossmann (CL0063) MCRA; MCRA family (PF06100; HMM-score: 746.4)
  and 2 more
  NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25)
  P-loop_NTPase (CL0023) AAA_15; AAA ATPase domain (PF13175; HMM-score: 15)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.031802
  • TAT(Tat/SPI): 0.005369
  • LIPO(Sec/SPII): 0.189365
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446213987 NCBI
• RefSeq: WP_000291842 NCBI
• UniProt: see SAUSA300_0108

⊟Protein sequence

• MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRDGQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAESIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDKFCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFTINRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKIEDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQKLALAGFLKKIKGTYIESLLKEHKLL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications