PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00760 [old locus tag: SAUSA300_0145 ]
• pan locus tag^?: SAUPAN000957000
• symbol: SAUSA300_RS00760
• pan gene symbol^?: phnD
• synonym:
• product: phosphonate ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00760 [old locus tag: SAUSA300_0145 ]
• symbol: SAUSA300_RS00760
• product: phosphonate ABC transporter substrate-binding protein
• replicon: chromosome
• strand: -
• coordinates: 163824..164780
• length: 957
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (163824..164780) NCBI
• BioCyc: SAUSA300_RS00760 BioCyc
• MicrobesOnline: see SAUSA300_0145

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGAAAAATTTTAAGTGTTTATTTGTATTAATGTTAGCAGTCATTGTTTTTGCAGCAGCA
  TGTGGAAACTCAAGTTCTTTAGATAATCAAAAGAACGCTAGTAATGATTCGGATTCTAAA
  TCAGGAGGATACAAACCTAAAGAATTAACCGTTCAATTTGTACCTTCGCAAAATGCTGGA
  ACATTAGAAGCTAAAGCAAAACCATTAGAAAAATTACTATCTAAAGAATTAGGGATTCCA
  GTTAAAGTGTCTGTATCAACTAACTACAATACAATTGTTGAAGCTATGAAGTCTAAAAAA
  GTTGATGTTGGTTTCTTACCACCAACGGCATACACATTAGCACATGATCAAAAAGCAGCT
  GATTTATTATTACAAGCACAACGTTTCGGTGTAAAAGAAGATGGTTCAGCAAGTAAAGAA
  CTTGTAGATAGTTATAAATCAGAAATTCTTGTTAAAAAAGACTCAAAAATTAAAAGCTTG
  AAAGATTTAAAAGGTAAGAAAATTGCCTTACAAGATGTAACATCAACTGCTGGATATACA
  TTCCCACTTGCGATGTTAAAAAACGAAGCAGGTATTAATGCAACTAAAGATATGAAAATT
  GTGAATGTTAAAGGTCATGACCAAGCAGTTATCTCATTATTAAATGGAGATGTAGATGCT
  GCGGCTGTATTTAACGATGCACGTAATACTGTGAAAAAAGACCAACCAAATGTATTTAAA
  GACACACGAATTTTAAAATTAACACAAGCTATTCCGAATGACACAATTTCTGTAAGACCA
  GATATGGATAAAGATTTTCAAGAAAAATTGAAAAAAGCTTTTATAGACATTGCTAAATCA
  AAAGAAGGTCACAAAATTATTAGCGAAGTTTATTCACATGAAGGATACACAGAAACGAAA
  GATTCAAATTTCGACATTGTAAGAGAGTACGAAAAATTAGTTAAAGATATGAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  957
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00760 [old locus tag: SAUSA300_0145 ]
• symbol: SAUSA300_RS00760
• description: phosphonate ABC transporter substrate-binding protein
• length: 318
• theoretical pI: 9.66321
• theoretical MW: 35000
• GRAVY: -0.393082

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein (TIGR01098; HMM-score: 257.2)
  and 6 more
  phosphonate ABC transporter, periplasmic phosphonate binding protein (TIGR03431; HMM-score: 173.6)
  putative selenate ABC transporter periplasmic binding protein (TIGR04553; HMM-score: 76.2)
  Metabolism Transport and binding proteins Other ABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 54.3)
  taurine ABC transporter, periplasmic binding protein (TIGR01729; HMM-score: 46.2)
  Metabolism Transport and binding proteins Unknown substrate TRAP transporter solute receptor, TAXI family (TIGR02122; HMM-score: 37.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 22.2)
• TheSEED: see SAUSA300_0145
• PFAM:
  PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 241.7)
  and 6 more
  NMT1; NMT1/THI5 like (PF09084; HMM-score: 48.4)
  SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 44.1)
  NMT1_2; NMT1-like family (PF13379; HMM-score: 24.1)
  Mycoplasma_p37; High affinity transport system protein p37 (PF06646; HMM-score: 23.8)
  NMT1_3; NMT1-like family (PF16868; HMM-score: 21.3)
  Hexosaminidase (CL0546) Glyco_hydro_20b; Glycosyl hydrolase family 20, domain 2 (PF02838; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.001797
  • TAT(Tat/SPI): 0.000106
  • LIPO(Sec/SPII): 0.997879
  • Cleavage Site: CS pos: 20-21. AAA-CG. Pr: 0.9982
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446710326 NCBI
• RefSeq: WP_000787672 NCBI
• UniProt: see SAUSA300_0145

⊟Protein sequence

• MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications