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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS00970 [old locus tag: SAUSA300_0185 ]
- pan locus tag?: SAUPAN001023000
- symbol: SAUSA300_RS00970
- pan gene symbol?: argJ
- synonym:
- product: bifunctional glutamate N-acetyltransferase/amino-acid N-acetyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS00970 [old locus tag: SAUSA300_0185 ]
- symbol: SAUSA300_RS00970
- product: bifunctional glutamate N-acetyltransferase/amino-acid N-acetyltransferase
- replicon: chromosome
- strand: -
- coordinates: 213404..214645
- length: 1242
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (213404..214645) NCBI
- BioCyc: SAUSA300_RS00970 BioCyc
- MicrobesOnline: see SAUSA300_0185
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGAAACATCAAGAAACGACATCACAACAATATAACTTTTCAATTATTAAACATGGCGAT
ATCAGTACACCTCAAGGCTTCACGGCTGGTGGTATGCACATCGGTTTACGCGCTAACAAA
AAAGACTTTGGATGGATTTACTCATCGTCTTTGGCAAGTGCAGCTGCCGTATATACTTTA
AATCAGTTTAAAGCTGCACCACTTATTGTCACTGAAGACACTTTACAAAAGTCTAAAGGA
AAATTACAAGCACTTGTTGTTAATTCAGCTAATGCAAATTCTTGTACCGGTCAACAAGGC
ATAGATGATGCACGACAAACACAAACATGGGTTGCTCAACAACTTCAAATACCATCTGAG
CATGTTGCTGTTGCTTCAACTGGGGTCATTGGTGAATATTTGCCTATGGATAAAATTAAG
ACTGGGACCGAACATATTAAGGATGCTAATTTTGCAACGCCAGGTGCGTTTAACGAGGCA
ATTTTAACAACTGATACCTGTACAAAACATATCGCTGTATCACTAAAAATCGATGGTAAA
ACCGTTACAATTGGTGGTAGCACCAAAGGTTCAGGTATGATTCACCCAAATATGGCTACC
ATGCTTGCTTTTATAACAACCGATGCATCGATTGAATCGAATACACTTCATCAATTATTA
AAATCTTCGACTGACCATACATTTAATATGATTACTGTTGATGGCGATACAAGTACAAAT
GACATGGTATTAGTCATGGCAAATCACCAAGTTGAACACCAAATACTTAGTCAAGACCAT
CCACAATGGGAAACATTTGTTGATGCATTCAATTTTGTCTGTACATTTTTAGCTAAAGCT
ATAGCCAGAGATGGCGAAGGCGCAACAAAGTTAATCAGCGTCAATGTGTCAGGCGCTAAA
TCAATATCAGATGCAAGAAAAATCGGTAAAACTATCGTAAGTTCAAATCTAGTAAAATCA
GCTATTTTTGGCGAAGATGCCAATTTTGGTCGAATCATTACAGCTATTGGCTACAGCGGA
TGTGAAATTGATCCTAACTGCACATATGTTCAACTGAACCAAATACCTGTCGTTGATAAA
GGTATGGCTGTACTATTTGATGAGCAAGCTATGTCGAATACATTAACTCATGAAAATGTC
ACAATTGACGTTCAGCTTGGTTTAGGTAACGCTGCAGCGACTGCATACGGTTGTGATTTA
TCCTATGATTATGTGCGTATCAACGCATCATATCGAACATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS00970 [old locus tag: SAUSA300_0185 ]
- symbol: SAUSA300_RS00970
- description: bifunctional glutamate N-acetyltransferase/amino-acid N-acetyltransferase
- length: 413
- theoretical pI: 6.18088
- theoretical MW: 44399.8
- GRAVY: -0.108232
⊟Function[edit | edit source]
- reaction: EC 2.3.1.1? ExPASyAmino-acid N-acetyltransferase Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamateEC 2.3.1.35? ExPASyGlutamate N-acetyltransferase N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
- TIGRFAM: Amino acid biosynthesis Glutamate family glutamate N-acetyltransferase/amino-acid acetyltransferase (TIGR00120; EC 2.3.1.1,2.3.1.35; HMM-score: 494.5)
- TheSEED: see SAUSA300_0185
- PFAM: DmpA_ArgJ (CL0635) ArgJ; ArgJ family (PF01960; HMM-score: 481.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.027663
- TAT(Tat/SPI): 0.010515
- LIPO(Sec/SPII): 0.002982
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446605265 NCBI
- RefSeq: WP_000682611 NCBI
- UniProt: see SAUSA300_0185
⊟Protein sequence[edit | edit source]
- MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFGWIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGIDDARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEAILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQLLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTFLAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIITAIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAAATAYGCDLSYDYVRINASYRT
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: ArgR see SAUSA300_0185
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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