PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS01180 [old locus tag: SAUSA300_0224 ]
• pan locus tag^?: SAUPAN001088000
• symbol: SAUSA300_RS01180
• pan gene symbol^?: coa
• synonym:
• product: coagulase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS01180 [old locus tag: SAUSA300_0224 ]
• symbol: SAUSA300_RS01180
• product: coagulase
• replicon: chromosome
• strand: +
• coordinates: 266403..268232
• length: 1830
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (266403..268232) NCBI
• BioCyc: SAUSA300_RS01180 BioCyc
• MicrobesOnline: see SAUSA300_0224

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAAAGCAAATAATTTCGCTAGGCGCATTAGCAGTTGCATCTAGCTTATTTACATGG
  GATAACAAAGCAGATGCGATAGTAACAAAGGATTATAGTGGGAAATCACAAGTTAATGCT
  GGGAGTAAAAATGGGACATTAATAGATAGCAGATATTTAAATTCAGCTCTATATTATTTG
  GAAGACTATATAATTTATGCTATAGGATTAACTAATAAATATGAATATGGAGATAATATT
  TATAAAGAAGCTAAAGATAGGTTGTTGGAAAAGGTATTAAGGGAAGATCAATATCTTTTG
  GAGAGAAAGAAATCTCAATATGAAGATTATAAACAATGGTATGCAAATTATAAAAAAGAA
  AATCCTCGTACAGATTTAAAAATGGCTAATTTTCATAAATATAATTTAGAAGAACTTTCG
  ATGAAAGAATACAATGAACTACAGGATGCATTAAAGAGAGCACTGGATGATTTTCACAGA
  GAAGTTAAAGATATTAAGGATAAGAATTCAGACTTGAAAACTTTTAATGCAGCAGAAGAA
  GATAAAGCAACTAAGGAAGTATACGATCTCGTATCTGAAATTGATACATTAGTTGTATCA
  TATTATGGTGATAAGGATTATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTA
  ATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACGTATTAAAAAAGAAATG
  ATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCG
  AAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAA
  CCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCT
  TGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAA
  GAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATAACCAACAAGAGGTTAAA
  ACTACGGCTGGTAAAGCTGAAGAAACAACACAACCAGTTGCACAACCATTAGTTAAAATT
  CCACAGGGCACAATTACAGGTGAAATTGTAAAAGGTCCGGAATATCCAACGATGGAAAAT
  AAAACGGTACAAGGTGAAATCGTTCAAGGTCCCGATTTTCTAACAATGGAACAAAGCGGC
  CCATCATTAAGCAATAATTATACAAACCCACCGTTAACGAACCCTATTTTAGAAGGTCTT
  GAAGGTAGCTCATCTAAACTTGAAATAAAACCACAAGGTACTGAATCAACGTTAAAAGGT
  ACTCAAGGAGAATCAAGTGATATTGAAGTTAAACCTCAAGCAACTGAAACAACAGAAGCT
  TCTCAATATGGTCCGAGACCGCAATTTAACAAAACACCTAAATATGTTAAATATAGAGAT
  GCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGA
  TTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTA
  TCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACA
  ACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAA
  ACAAATGCATACAACGTAACAACACATGCAAACGGTCAAGTGTCATACGGAGCTCGCCCG
  ACATACAAGAAGCCAAGTAAAACAAATGCATACAATGTAACAACACATGCAGATGGTACT
  GCGACATATGGGCCTAGAGTAACAAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS01180 [old locus tag: SAUSA300_0224 ]
• symbol: SAUSA300_RS01180
• description: coagulase
• length: 609
• theoretical pI: 8.58401
• theoretical MW: 68779.9
• GRAVY: -1.03957

⊟Function

• TIGRFAM:
  two transmembrane protein (TIGR04527; HMM-score: 16.9)
• TheSEED: see SAUSA300_0224
• PFAM:
  no clan defined Coagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 451.9)
  and 3 more
  Staphylcoagulse; Staphylocoagulase repeat (PF04022; HMM-score: 221.7)
  Ntox25; Bacterial toxin 25 (PF15530; HMM-score: 12.4)
  Zn_Beta_Ribbon (CL0167) DUF1451; Zinc-ribbon containing domain (PF07295; HMM-score: 9.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.982814
  • TAT(Tat/SPI): 0.001993
  • LIPO(Sec/SPII): 0.001993
  • Cleavage Site: CS pos: 26-27. ADA-IV. Pr: 0.9133
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446666727 NCBI
• RefSeq: WP_000744073 NCBI
• UniProt: see SAUSA300_0224

⊟Protein sequence

• MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKNGTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLERKKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHREVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKLDLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTNSDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVDNQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPDFLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SAUSA300_0224

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications