PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS01510 [old locus tag: SAUSA300_0282 ]
• pan locus tag^?: SAUPAN001179000
• symbol: SAUSA300_RS01510
• pan gene symbol^?: essB
• synonym:
• product: protein EssB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS01510 [old locus tag: SAUSA300_0282 ]
• symbol: SAUSA300_RS01510
• product: protein EssB
• replicon: chromosome
• strand: +
• coordinates: 334612..335946
• length: 1335
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (334612..335946) NCBI
• BioCyc: SAUSA300_RS01510 BioCyc
• MicrobesOnline: see SAUSA300_0282

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
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  661
  721
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  841
  901
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  1021
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  1141
  1201
  1261
  1321
  
  ATGGTTAAAAATCATAACCCTAAAAATGAAATGCAAGATATGTTAACGCCTTTAGATGCT
  GAAGAAGCAGCTAAAACAAAATTACGCTTAGATATGAGAGAGATTCCTAAGTCTTCAATT
  AAACCAGAACATTTTCATTTAATGTACTTATTAGAACAACATTCTCCATATTTTATAGAT
  GCTGAATTAACTGAACTACGTGACAGTTTCCAAATACATTATGACATTAATGACAATCAT
  ACACCTTTTGATAATATTAAATCATTTACTAAAAATGAAAAATTACGTTACTTACTCAAT
  ATCAAAAATTTAGAAGAAGTAAATCGTACACGCTACACATTTGTGTTGGCACCAGATGAA
  TTATTTTTCACAAGAGATGGATTACCCATTGCTAAAACAAGAGGGTTACAAAATGTTGTT
  GATCCATTACCTGTGTCAGAAGCTGAATTTTTAACAAGATATAAAGCGCTGGTTATCTGT
  GCATTCAATGAGAAACAATCATTTGATGCTTTAGTTGAAGGAAACTTAGAACTACATAAA
  GGAACGCCATTTGAAACTAAAGTTATTGAAGCGGCAACGTTAGATTTACTAACGGCATTT
  TTAGATGAACAGTATCAGAAACAAGAACAAGATTATAGTCAAAATTATGCATATGTACGC
  AAAGTAGGACATACCGTTTTCAAATGGGTTGCTATCGGTATGACAACGTTAAGTGTTTTA
  TTAATTGCATTCTTAGCCTTTTTATATTTTTCAGTAATGAAGCATAATGAGCGCATTGAA
  AAAGGATACCAAGCATTTGTAAAGGATGATTATACGCAAGTACTAAATACGTATGATGAT
  TTAGATGGTAAAAAATTAGATAAAGAGGCACTTTACATTTATGCCAAAAGTTATATCCAA
  ACAAATAAACAAGGTTTAGAAAAAGATAAGAAAGAAAATTTACTTAATAATGTGACACCA
  AATTCAAACAAAGACTACTTATTATATTGGATGGAATTAGGACAAGGACATCTTGATGAA
  GCGATTAATATTGCCACTTATTTAGATGATAACGATATTACAAAGTTAGCGTTGATTAAT
  AAATTAAATGAGATTAAAAATAACGGAGATTTATCGAATGATAAACGTTCTGAAGAAACG
  AAAAAGTATAACGATAAATTGCAAGATATTTTAGACAAAGAAAAACAAGTTAAAGATGAA
  AAAGCGAAATCTGAAGAAGAGAAAGCAAAAGCGAAAGATGAGAAATTAAAGCAACAAGAA
  GAGAACGAAAAGAAACAAAAAGAACAAGCACAAAAAGATAAAGAAAAACGCCAAGAAGCT
  GAAAGAAAAAAATAG
  60
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⊟Protein

• 4ANN
  PDB

Protein Data Bank: 4ANN

⊟General

• locus tag: SAUSA300_RS01510 [old locus tag: SAUSA300_0282 ]
• symbol: SAUSA300_RS01510
• description: protein EssB
• length: 444
• theoretical pI: 5.74096
• theoretical MW: 52023.5
• GRAVY: -0.834009

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssB (TIGR03926; HMM-score: 452.3)
  and 2 more
  Cellular processes Cellular processes Pathogenesis protein TolA (TIGR02794; HMM-score: 4.5)
  Metabolism Transport and binding proteins Other protein TolA (TIGR02794; HMM-score: 4.5)
• TheSEED: see SAUSA300_0282
• PFAM:
  PKinase (CL0016) YukC; WXG100 protein secretion system (Wss), protein YukC (PF10140; HMM-score: 401.3)
  and 5 more
  no clan defined DUF2417; Region of unknown function (DUF2417) (PF10329; HMM-score: 12.5)
  FTCD_N; Formiminotransferase domain, N-terminal subdomain (PF07837; HMM-score: 9.1)
  Silic_transp; Silicon transporter (PF03842; HMM-score: 8.1)
  U79_P34; HSV U79 / HCMV P34 (PF03064; HMM-score: 5.7)
  DUF4175; Domain of unknown function (DUF4175) (PF13779; HMM-score: 4.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003941
  • TAT(Tat/SPI): 0.000206
  • LIPO(Sec/SPII): 0.000443
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446162483 NCBI
• RefSeq: WP_000240338 NCBI
• UniProt: see SAUSA300_0282

⊟Protein sequence

• MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEHFHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIKNLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVICAFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAYVRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLNTYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELGQGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILDKEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications