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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS01825 [old locus tag: SAUSA300_0344 ]
- pan locus tag?: SAUPAN001882000
- symbol: SAUSA300_RS01825
- pan gene symbol?: fepA
- synonym:
- product: EfeM/EfeO family lipoprotein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS01825 [old locus tag: SAUSA300_0344 ]
- symbol: SAUSA300_RS01825
- product: EfeM/EfeO family lipoprotein
- replicon: chromosome
- strand: +
- coordinates: 396024..396878
- length: 855
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (396024..396878) NCBI
- BioCyc: SAUSA300_RS01825 BioCyc
- MicrobesOnline: see SAUSA300_0344
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGAAAAAGTTAACAACGCTATTATTAGCATCAACGTTATTAATTGCTGCATGTGGGAAC
GACGATAGTAAGAAGGATGATTCAAAGACATCGAAAAAAGATGATGGTGTTAAAGCAGAA
TTAAAACAAGCAACAAAAGCATATGATAAATATACTGATGAACAGTTAAATGAATTTTTA
AAAGGTACAGAAAAATTTGTTAAAGCGATTGAAAATAATGATATGGCCCAAGCAAAAGCG
TTATATCCAAAAGTTCGTATGTATTATGAACGCTCTGAACCAGTTGCAGAAGCATTTGGA
GATTTAGATCCTAAAATTGATGCACGTCTTGCAGATATGAAAGAAGAGAAAAAGGAAAAA
GAATGGTCAGGATATCATAAGATTGAAAAAGCATTATACGAAGATAAGAAAATTGATGAT
GTGACTAAAAAAGATGCACAACAATTATTGAAAGATGCAAAAGAATTGCATGCCAAAGCT
GATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAA
GTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGAT
TTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAG
AAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTA
TTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCATTTGAAAAAGTATCGAAGAAA
GACCGTAAAGCATTTGCGGATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCT
GTGATTACTGAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS01825 [old locus tag: SAUSA300_0344 ]
- symbol: SAUSA300_RS01825
- description: EfeM/EfeO family lipoprotein
- length: 284
- theoretical pI: 5.23329
- theoretical MW: 32258.5
- GRAVY: -0.790493
⊟Function[edit | edit source]
- TIGRFAM: XXXCH domain (TIGR04358; HMM-score: 16.9)
- TheSEED: see SAUSA300_0344
- PFAM: no clan defined Peptidase_M75; Imelysin (PF09375; HMM-score: 125)and 4 moreLipase_bact_N; Bacterial virulence factor lipase N-terminal (PF12262; HMM-score: 16)PCYCGC; Protein of unknown function with PCYCGC motif (PF13798; HMM-score: 15.1)LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 11.8)Transporter (CL0375) Pannexin_like; Pannexin-like TM region of LRRC8 (PF12534; HMM-score: 11.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 4.6
- Cellwall Score: 2.48
- Extracellular Score: 2.92
- Internal Helices: 0
- LocateP:
- SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
- SP(Sec/SPI): 0.00048
- TAT(Tat/SPI): 0.000073
- LIPO(Sec/SPII): 0.999161
- Cleavage Site: CS pos: 17-18. IAA-CG. Pr: 0.9997
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446659847 NCBI
- RefSeq: WP_000737193 NCBI
- UniProt: see SAUSA300_0344
⊟Protein sequence[edit | edit source]
- MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQATKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDLDPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKADTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPILEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPLSKMAVITE
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: Fur* see SAUSA300_0344
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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