COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS02205 [old locus tag: SAUSA300_0411 ]
• pan locus tag^?:
• symbol: SAUSA300_RS02205
• pan gene symbol^?: —
• synonym:
• product: tandem-type lipoprotein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS02205 [old locus tag: SAUSA300_0411 ]
• symbol: SAUSA300_RS02205
• product: tandem-type lipoprotein
• replicon: chromosome
• strand: +
• coordinates: 464429..465229
• length: 801
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (464429..465229) NCBI
• BioCyc: SAUSA300_RS02205 BioCyc
• MicrobesOnline: see SAUSA300_0411

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCGATATCTAAAAAAGCTTGCATGGTTCATAAGTGTTATTATTTTGGGCATTTTTATA
  ATAGGTTGTGATAGTTCAAGCGATACTGGGGAAAAAGCAAAAGAAGATTCAAAGGAAGAA
  CAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGAC
  TTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGG
  ATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATG
  ATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATA
  TATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAAAACAATAAA
  ATTATTTTATTAGACAAGGTGGAAGATCAAAAACTTAAAGAAAGAATAGAAAACTTTAAA
  TTTTTCGGACAATATGCCGATTTCAAGAGTTTGAAAAGTTACAACAATGGCGACGTTTCA
  ATTAATAGTAATGTTCCAAGTTATGACGCGAAATTTAAAATGAGTAATAAAGATGAAAAT
  GTTAAGCAATTAAGAAGTCGTTATAACATTCCTACTGAAAAAGCTCCAATATTAAAAATG
  CATATTGATGGGGACTTAAAAGGCAGTTCCGTTGGATATAAAAAGTTAGAAATAGACTTT
  TCAAAAGAAGAAAATAGCGAATTATCAGTAGTCGATTCATTAAATTTTCAGCCTGCCAAA
  AAAAATAAAGATGATGAATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS02205 [old locus tag: SAUSA300_0411 ]
• symbol: SAUSA300_RS02205
• description: tandem-type lipoprotein
• length: 266
• theoretical pI: 8.27828
• theoretical MW: 30916.8
• GRAVY: -0.844737

⊟Function

• TIGRFAM:
  Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 386.8)
• TheSEED: see SAUSA300_0411
• PFAM:
  LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 368.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
  • SP(Sec/SPI): 0.000581
  • TAT(Tat/SPI): 0.000045
  • LIPO(Sec/SPII): 0.999221
  • Cleavage Site: CS pos: 22-23. IIG-CD. Pr: 0.9996
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 447192503 NCBI
• RefSeq: WP_001269759 NCBI
• UniProt: see SAUSA300_0411

⊟Protein sequence

• MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKKSFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMILNLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFKFFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPILKMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SAUSA300_0411

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications