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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0411 [new locus tag: SAUSA300_RS02205 ]
  • pan locus tag?:
  • symbol: SAUSA300_0411
  • pan gene symbol?:
  • synonym:
  • product: tandem lipoprotein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0411 [new locus tag: SAUSA300_RS02205 ]
  • symbol: SAUSA300_0411
  • product: tandem lipoprotein
  • replicon: chromosome
  • strand: +
  • coordinates: 464429..465229
  • length: 801
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCGATATCTAAAAAAGCTTGCATGGTTCATAAGTGTTATTATTTTGGGCATTTTTATA
    ATAGGTTGTGATAGTTCAAGCGATACTGGGGAAAAAGCAAAAGAAGATTCAAAGGAAGAA
    CAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGAC
    TTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGG
    ATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATG
    ATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATA
    TATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAAAACAATAAA
    ATTATTTTATTAGACAAGGTGGAAGATCAAAAACTTAAAGAAAGAATAGAAAACTTTAAA
    TTTTTCGGACAATATGCCGATTTCAAGAGTTTGAAAAGTTACAACAATGGCGACGTTTCA
    ATTAATAGTAATGTTCCAAGTTATGACGCGAAATTTAAAATGAGTAATAAAGATGAAAAT
    GTTAAGCAATTAAGAAGTCGTTATAACATTCCTACTGAAAAAGCTCCAATATTAAAAATG
    CATATTGATGGGGACTTAAAAGGCAGTTCCGTTGGATATAAAAAGTTAGAAATAGACTTT
    TCAAAAGAAGAAAATAGCGAATTATCAGTAGTCGATTCATTAAATTTTCAGCCTGCCAAA
    AAAAATAAAGATGATGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    801

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0411 [new locus tag: SAUSA300_RS02205 ]
  • symbol: SAUSA300_0411
  • description: tandem lipoprotein
  • length: 266
  • theoretical pI: 8.27828
  • theoretical MW: 30916.8
  • GRAVY: -0.844737

Function[edit | edit source]

  • TIGRFAM:
    Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 386.8)
  • TheSEED  :
    • Tandem lipoprotein within Pathogenicity island
  • PFAM:
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 368.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: IFIIGCD
  • SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.000581
    • TAT(Tat/SPI): 0.000045
    • LIPO(Sec/SPII): 0.999221
    • Cleavage Site: CS pos: 22-23. IIG-CD. Pr: 0.9996
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKKSFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMILNLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFKFFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPILKMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]