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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02285 [old locus tag: SAUSA300_0426 ]
  • pan locus tag?: SAUPAN002164000
  • symbol: SAUSA300_RS02285
  • pan gene symbol?: mpsB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02285 [old locus tag: SAUSA300_0426 ]
  • symbol: SAUSA300_RS02285
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 479079..481784
  • length: 2706
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACAACACAGTTAAATATCAATTCAGTCATTGAAAATGCGAAACGTGTTATTACACCA
    TTATCACCAATTTCGATTTTTGCAGCACGAAATCCATGGGAAGGATTAGAAGCGGATACG
    TTTGAAGATGTCGCAAAATGGTTACGTGATGTTCGAGATGTGGATATTTTCCCAAATAAA
    GCATTAATAGAAAGCGCTGTGGCACGTGGTGAATTAGATGAAAGTGTCTTTAATCAACTT
    GTTACTGATATGTTACTTGAACATCACTACAATATCCCGCAACACTACATCAATCTTTAT
    ATTGATAACATTAAAACATTAAAAGACGTACCTGCATCATATATGAATCATTCAAATGTT
    GATGTTGTTGCTGATCTACTATTAGAAAAATCAAAACGTGATATGGCTGAATCATATCAT
    CACTATGATGTACGTCCGATGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGC
    GAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCG
    AGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAA
    CATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCT
    GAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAGCT
    TATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCA
    CAACAGCATTACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTC
    GAACAATTGCTAGTTGGTGATGAGTTTAAGTCAGTCGCTAAAGATTGTGAAAGTAGATCG
    GAAAATTGGTTTAAGCAAACTGTTGCATCATGGTGTTACTACAGTGATATGCCTAGCGAT
    GTATTACTACAACATGACGTCAATGAAATTCAAACGTTTATTCATTTTGCAGCAACTATG
    AATAAAAATGTATTTAAAAATTTATGGCTAATTGCCTGGGAAATGACATACGAATCTCAG
    TTAAAACAAAAAATTAAAGCAGGTCATGAAAGTGTGGCGGGCGCATTAGATGTAAACCAA
    GTAAATGTCTCAGAAAATGATAACGCTAATCAGCCACATTCAGTATTGTTAAATGACACA
    CAAGCAGTTGATGAAAATAATAGCGAGCTAAATCAGATGGGCACATCAACGAAAGCGCAA
    ATTGCATTTTGTATAGATGTTCGTTCAGAACCATTTCGTAGACATATCGAAGCAGCAGGG
    CCCTTTGAAACGATTGGTATTGCAGGCTTCTTTGGATTACCTATTCAAAAAGATGCCGTA
    GACGAACAATTCAAACATGATTCATTACCTGTCATGGTACCGCCGGCATATCGCATTAAA
    GAATTTGCAGACCGCTACGATATGAATGTTTATCGACAACAGCAACAGACAATGTCATCG
    ATGTTTTACACATTTAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAA
    TTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGT
    CGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACG
    ATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAA
    ATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAGCATTTGCGCCGTTC
    GTTGTGTTAGCAGGTCATGCTAGTCATTCTCACAATAATCCACATCATGCATCACTTGAA
    TGTGGGGCTTGTGGTGGCGCATCAAGCGGTTTTAATGCTAAGTTATTAGCGATGATATGT
    AATCGTCCAAATGTCAGACAAGGATTAAAACAATCAGGTGTGTATATTCCAGAGACAACT
    GTTTTTGCGGTAGCAGAACATCATACGTCTACTGATACGTTGGCATGGGTATATGTGCCA
    GACACATTATCTTCTATTGCTTTAGATGCATATGAATCATTGAATGACGCGATGCCGATG
    ATTTCTGAACACGCGAATCGCGAACGTTTGGACAAACTGCCAACGATTGGTCGTGTGAAT
    CATCCAGTGGAAGAAGCGCAGCGGTTTGCGAGTGATTGGAGTGAGGTACGTCCAGAATGG
    GGATTGGCTAAAAATGCATCATTTATAATTGGACGACGCCAATTAACAAAAGGCATTGAT
    TTAGAAGGGCGGACATTTTTACACAATTATGATTGGCGTAAAGATAAAGATGGCACATTA
    TTAAATACCATCATTTCTGGTCCGGCACTTGTGGCACAATGGATTAATTTACAATATTAT
    GCGTCGACAGTTGCGCCGCATTTTTACGGAAGTGGGAATAAAGCGACACAAACCGTCACG
    TCAGGTGTTGGTGTCATGCAAGGTAATGCGAGTGATCTGATGTATGGCTTATCATGGCAA
    TCTGTTATGGCTGCTGATCGGACGATGTATCATTCGCCAATTCGTTTACTTGTCGTTATT
    CAGGCACCCGACTATGTTGTAGCAAGACTACTCGCGAATAATGAGCATTTCGCTAGGAAG
    GTGTCTAATCATTGGCTGCGTTTAATGAGCGTTAATGAGGAAGGGCGTTTTAAAAGTTGG
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2706

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS02285 [old locus tag: SAUSA300_0426 ]
  • symbol: SAUSA300_RS02285
  • description: hypothetical protein
  • length: 901
  • theoretical pI: 6.10957
  • theoretical MW: 102643
  • GRAVY: -0.330078

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, small subunit (TIGR02084; EC 4.2.1.33; HMM-score: 11.6)
  • TheSEED: see SAUSA300_0426
  • PFAM:
    no clan defined DUF2309; Uncharacterized protein conserved in bacteria (DUF2309) (PF10070; HMM-score: 848.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024395
    • TAT(Tat/SPI): 0.000633
    • LIPO(Sec/SPII): 0.002325
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDVAKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYIDNIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLSEQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPNDPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAIRLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFIHFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQPHSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGFFGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLMKNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFDRTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGACGGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDTLSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEWGLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQYYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRLLVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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