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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02385 [old locus tag: SAUSA300_0446 ]
  • pan locus tag?: SAUPAN002187000
  • symbol: SAUSA300_RS02385
  • pan gene symbol?: gltD
  • synonym:
  • product: glutamate synthase subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02385 [old locus tag: SAUSA300_0446 ]
  • symbol: SAUSA300_RS02385
  • product: glutamate synthase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 501919..503382
  • length: 1464
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTGAATTTAAAGGATTTATGAAGTATGACAAACAGTACTTAGGTGAATTATCACTG
    GTAGACCGTTTGAAGCATCATAAAGCATATCAACAACGATTTACTAAAGAAGATGCCTCT
    ATCCAAGGTGCACGATGTATGGATTGTGGAACGCCGTTTTGTCAAACCGGACAACAGTAT
    GGTAGGGAAACAATAGGTTGTCCAATTGGAAACTACATTCCTGAATGGAACGACTTAGTG
    TATCATCAAGATTTTAAAACTGCTTATGAACGCTTAAGCGAAACAAATAACTTTCCTGAC
    TTTACAGGGCGTGTATGTCCTGCACCATGCGAAAGTGCTTGTGTGATGAAGATTAATAGA
    GAATCGATTGCGATTAAAGGTATTGAACGCACAATTATTGATGAAGCTTTTGAAAATGGT
    TGGGTAGCGCCGAAAGTTCCGAGTCGCCGTAGAGATGAAAAAGTGGCAATCGTTGGAAGC
    GGTCCAGCAGGATTAGCTGCTGCTGAAGAACTTAATCTACTAGGATATCAAGTAACTATT
    TATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTGTGGTATTCCGAATATGAAACTT
    GATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATT
    AATGGTGTTGAAGTCGGCGTTGATATTGATAAAGCAACGTTAGAATCTGAGTATGATGCC
    ATTATATTATGTACTGGTGCACAAAAAGGTAGAGATTTACCTTTAGAAGGACGCATGGGT
    GATGGTATACATTTCGCTATGGATTATTTAACTGAACAAACGCAGTTGTTAAATGGAGAA
    ATTGATGATATAACAATAACTGCAAAAGATAAGAATGTCATTATCATTGGTGCTGGTGAT
    ACAGGGGCAGACTGTGTAGCGACAGCATTAAGAGAAAATTGTAAATCGATTGTTCAATTT
    AATAAATATACGAAATTGCCAGAAGCAATTACATTTACAGAAAATGCATCATGGCCTTTA
    GCAATGCCGGTGTTTAAAATGGACTATGCGCACCAAGAGTACGAAGCTAAGTTTGGTAAG
    GAACCACGTGCATATGGTGTTCAAACAATGCGTTACGATGTTGACGATAAAGGACACATA
    CGTGGTTTGTATACTCAAATTTTAGAGCAAGGCGAAAATGGTATGGTCATGAAAGAAGGA
    CCTGAAAGATTTTGGCCTGCTGACCTTGTATTATTATCAATCGGCTTCGAAGGTACAGAA
    CCAACAGTACCGAATGCTTTTAACATTAAAACGGATAGAAATCGAATCGTGGCGGATGAT
    ACAAACTATCAAACTAATAATGAAAAGGTATTTGCTGCTGGAGATGCTAGACGTGGTCAA
    AGTTTAGTTGTATGGGCAATTAAAGAAGGTAGAGGCGTAGCGAAAGCAGTAGATCAGTAT
    TTAGCTAGTAAAGTTTGTGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1464

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS02385 [old locus tag: SAUSA300_0446 ]
  • symbol: SAUSA300_RS02385
  • description: glutamate synthase subunit beta
  • length: 487
  • theoretical pI: 5.04053
  • theoretical MW: 54367.5
  • GRAVY: -0.378439

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.13?  ExPASy
    Glutamate synthase (NADPH) 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH
  • TIGRFAM:
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 607.8)
    and 35 more
    glutamate synthase, small subunit (TIGR01318; HMM-score: 282)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 221.2)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 160)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 58.8)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 46.2)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 44)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 38.8)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 34.2)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 30.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02028; HMM-score: 27.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 25.9)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 25.2)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 25)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 24.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 23.8)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 20.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 20.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 20)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 19.6)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 18.8)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 18.7)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 18.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 17.5)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 16.9)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 16.8)
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 13.3)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 13.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 12.7)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 12.7)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 12.2)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 12.1)
    lycopene cyclase family protein (TIGR01790; HMM-score: 11.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 11.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 11.7)
  • TheSEED: see SAUSA300_0446
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 102.2)
    4Fe-4S (CL0344) Fer4_20; Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster (PF14691; HMM-score: 83)
    and 23 more
    NADP_Rossmann (CL0063) DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 34.5)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 32)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 32)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 28.5)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 26.5)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 25.6)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 23.6)
    Thi4; Thi4 family (PF01946; HMM-score: 23.3)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 23.3)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 20.9)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.5)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 19.7)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 19.3)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 18.6)
    FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 17.2)
    YjeF_N; YjeF-related protein N-terminus (PF03853; HMM-score: 16.3)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 16.3)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 14)
    no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 13.6)
    NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 13.5)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 12.2)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 11.8)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.069056
    • TAT(Tat/SPI): 0.00348
    • LIPO(Sec/SPII): 0.003297
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGSGPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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