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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS02970 [old locus tag: SAUSA300_0557 ]
- pan locus tag?: SAUPAN002349000
- symbol: SAUSA300_RS02970
- pan gene symbol?: —
- synonym:
- product: HAD family hydrolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS02970 [old locus tag: SAUSA300_0557 ]
- symbol: SAUSA300_RS02970
- product: HAD family hydrolase
- replicon: chromosome
- strand: +
- coordinates: 630185..630832
- length: 648
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (630185..630832) NCBI
- BioCyc: SAUSA300_RS02970 BioCyc
- MicrobesOnline: see SAUSA300_0557
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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601TTGAAGTTTGACAATTATATTTTTGATTTTGATGGTACGTTGGCAGACACGAAAAAATGT
GGTGAAGTAGCAACACAAAGTGCATTTAAAGCATGTGGCTTAACGGAACCATCATCTAAA
GAAATAACGCATTATATGGGAATACCTATTGAAGAATCATTTTTAAAATTAGCAGACCGA
CCATTAGATGAAGCAGCATTAGCAAAGTTAATCGATACATTTAGACATACATATCAATCT
ATTGAAAAGGACTATATTTATGAATTTGCGGGTATAACTGAAGCCATTACAAGTTTGTAT
AACCAAGGGAAAAAACTTTTCGTGGTGTCTAGTAAGAAGAGTGATGTATTAGAAAGAAAT
TTATCGGCTATTGGATTAAATCACTTGATTACCGAAGCTGTTGGATCCGATCAAGTAAGT
GCATATAAACCAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGC
CAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGT
ATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAAT
CCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS02970 [old locus tag: SAUSA300_0557 ]
- symbol: SAUSA300_RS02970
- description: HAD family hydrolase
- length: 215
- theoretical pI: 4.79367
- theoretical MW: 23866.6
- GRAVY: -0.26186
⊟Function[edit | edit source]
- TIGRFAM: Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 94)AHBA synthesis associated protein (TIGR01454; HMM-score: 77.9)and 35 moreHAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 69.3)phosphonatase-like hydrolase (TIGR03351; HMM-score: 66.4)beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 57)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 52.6)HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 48.3)haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 46.4)Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 44.6)Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 41.5)noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 38.6)Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 34.1)HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 33.7)Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 32)histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 29.9)Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 29.2)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 27.1)Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 26.1)HAD phosphatase, family IIIC (TIGR01681; HMM-score: 25.3)Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 24.2)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 24.1)phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 23.4)HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 23.2)pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 22.7)phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 19.8)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 19.2)FkbH domain (TIGR01686; HMM-score: 18.7)DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 18.2)Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 17.3)Central intermediary metabolism Other 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (TIGR01489; HMM-score: 16.9)HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 16)epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 14.6)phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14.2)Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 13.8)HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 13)Amino acid biosynthesis Histidine family histidinol-phosphatase (TIGR01261; EC 3.1.3.15; HMM-score: 12.5)sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 12.3)
- TheSEED: see SAUSA300_0557
- PFAM: HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 153.8)and 5 moreHydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 51.3)Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 49.6)HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 37.9)Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 29.5)PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 15.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.012731
- TAT(Tat/SPI): 0.000669
- LIPO(Sec/SPII): 0.010902
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446589751 NCBI
- RefSeq: WP_000667097 NCBI
- UniProt: see SAUSA300_0557
⊟Protein sequence[edit | edit source]
- MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITHYMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQGKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNSQQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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