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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus USA300_FPR3757
locus tag: SAUSA300_RS03065 [old locus tag: SAUSA300_0573 ]
pan locus tag^?: SAUPAN002372000
symbol: SAUSA300_RS03065
pan gene symbol^?: mvaD
synonym:
product: diphosphomevalonate decarboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SAUSA300_RS03065 [old locus tag: SAUSA300_0573 ]
symbol: SAUSA300_RS03065
product: diphosphomevalonate decarboxylase
replicon: chromosome
strand: +
coordinates: 647616..648599
length: 984
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_007793 (647616..648599) NCBI
BioCyc: SAUSA300_RS03065 BioCyc
MicrobesOnline: see SAUSA300_0573

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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961
TTGATTAAAAGTGGCAAAGCACGTGCACATACGAATATTGCACTTATAAAATATTGGGGT
AAAAAAGATGAAGCACTAATCATTCCAATGAATAATAGCATATCTGTTACATTAGAAAAA
TTTTACACTGAAACGAAAGTCACTTTTAACGACCAGTTAACACAGGATCAATTTTGGTTG
AATGGTGAAAAGGTTAGTGGCAAAGAATTAGAGAAAATTTCAAAATATATGGATATTGTC
AGAAATAGAGCTGGCATCGATTGGTATGCAGAAATTGAAAGCGACAATTTTGTACCAACA
GCAGCAGGGTTGGCTTCATCGGCAAGCGCATATGCAGCTTTAGCAGCAGCTTGTAATCAA
GCGCTAGACATGCAGCTGTCAGATAAGGATTTATCGAGATTGGCGCGAATTGGTTCGGGT
TCTGCGTCGCGTAGTATTTATGGTGGATTTGCAGAATGGGAAAAAGGGTATAGTGATGAG
ACGTCATATGCCGTTCCACTTGAATCGAATCATTTTGAAGATGACCTTGCCATGATATTT
GTTGTGATTAATCAACATTCTAAAAAGGTACCTAGTCGATATGGTATGTCATTGACACGA
AACACATCAAGGTTTTATCAATATTGGTTAGATCATATTGATGAAGATTTAGCTGAAGCA
AAAGCAGCGATTCAAGACAAAGATTTTAAACGCCTTGGTGAAGTAATTGAAGAAAATGGT
TTGCGTATGCATGCCACGAATCTAGGATCAACACCGCCGTTCACATATCTTGTGCAAGAA
AGTTATGATGTCATGGCGCTTGTTCACGAATGCCGAGAAGCGGGGTATCCGTGTTATTTT
ACAATGGATGCGGGACCTAATGTGAAAATACTTGTAGAAAAGAAAAACAAGCAACAGATT
ATAGATAAATTATTAACACAGTTTGATAATAACCAAATTATTGATAGTGACATTATTGCC
ACAGGAATTGAAATAATTGAGTAA
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984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SAUSA300_RS03065 [old locus tag: SAUSA300_0573 ]
symbol: SAUSA300_RS03065
description: diphosphomevalonate decarboxylase
length: 327
theoretical pI: 4.83909
theoretical MW: 36822.3
GRAVY: -0.34893

⊟Function[edit | edit source]

reaction:
EC 4.1.1.33? ExPASy
Diphosphomevalonate decarboxylase ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO₂
TIGRFAM:
Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 398.5)
and 5 more
Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 45.1)
Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 25.2)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 16.9)
Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 16.2)
Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 16)
TheSEED: see SAUSA300_0573
PFAM:
S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 31.9)
no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 30.2)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004285
- TAT(Tat/SPI): 0.000159
- LIPO(Sec/SPII): 0.000933
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446519989 NCBI
RefSeq: WP_000597335 NCBI
UniProt: see SAUSA300_0573

⊟Protein sequence[edit | edit source]

MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTETKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAAGLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDETSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLAEAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGYPCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

data available for COL, NCTC8325, Newman

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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