PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0573 [new locus tag: SAUSA300_RS03065 ]
• pan locus tag^?: SAUPAN002372000
• symbol: mvaD
• pan gene symbol^?: mvaD
• synonym:
• product: mevalonate diphosphate decarboxylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0573 [new locus tag: SAUSA300_RS03065 ]
• symbol: mvaD
• product: mevalonate diphosphate decarboxylase
• replicon: chromosome
• strand: +
• coordinates: 647616..648599
• length: 984
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915326 NCBI
• RefSeq: YP_493276 NCBI
• BioCyc: see SAUSA300_RS03065
• MicrobesOnline: 1292088 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  TTGATTAAAAGTGGCAAAGCACGTGCACATACGAATATTGCACTTATAAAATATTGGGGT
  AAAAAAGATGAAGCACTAATCATTCCAATGAATAATAGCATATCTGTTACATTAGAAAAA
  TTTTACACTGAAACGAAAGTCACTTTTAACGACCAGTTAACACAGGATCAATTTTGGTTG
  AATGGTGAAAAGGTTAGTGGCAAAGAATTAGAGAAAATTTCAAAATATATGGATATTGTC
  AGAAATAGAGCTGGCATCGATTGGTATGCAGAAATTGAAAGCGACAATTTTGTACCAACA
  GCAGCAGGGTTGGCTTCATCGGCAAGCGCATATGCAGCTTTAGCAGCAGCTTGTAATCAA
  GCGCTAGACATGCAGCTGTCAGATAAGGATTTATCGAGATTGGCGCGAATTGGTTCGGGT
  TCTGCGTCGCGTAGTATTTATGGTGGATTTGCAGAATGGGAAAAAGGGTATAGTGATGAG
  ACGTCATATGCCGTTCCACTTGAATCGAATCATTTTGAAGATGACCTTGCCATGATATTT
  GTTGTGATTAATCAACATTCTAAAAAGGTACCTAGTCGATATGGTATGTCATTGACACGA
  AACACATCAAGGTTTTATCAATATTGGTTAGATCATATTGATGAAGATTTAGCTGAAGCA
  AAAGCAGCGATTCAAGACAAAGATTTTAAACGCCTTGGTGAAGTAATTGAAGAAAATGGT
  TTGCGTATGCATGCCACGAATCTAGGATCAACACCGCCGTTCACATATCTTGTGCAAGAA
  AGTTATGATGTCATGGCGCTTGTTCACGAATGCCGAGAAGCGGGGTATCCGTGTTATTTT
  ACAATGGATGCGGGACCTAATGTGAAAATACTTGTAGAAAAGAAAAACAAGCAACAGATT
  ATAGATAAATTATTAACACAGTTTGATAATAACCAAATTATTGATAGTGACATTATTGCC
  ACAGGAATTGAAATAATTGAGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  984
  

⊟Protein

⊟General

• locus tag: SAUSA300_0573 [new locus tag: SAUSA300_RS03065 ]
• symbol: MvaD
• description: mevalonate diphosphate decarboxylase
• length: 327
• theoretical pI: 4.83909
• theoretical MW: 36822.3
• GRAVY: -0.34893

⊟Function

• reaction:
  EC 4.1.1.33?  ExPASy
  Diphosphomevalonate decarboxylase ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO₂
• TIGRFAM:
  Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 398.5)
  and 5 more
  Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 45.1)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 25.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 16.9)
  Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 16.2)
  Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 16)
• TheSEED  :
  • Diphosphomevalonate decarboxylase (EC 4.1.1.33)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Diphosphomevalonate decarboxylase (EC 4.1.1.33)
  and 1 more
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Diphosphomevalonate decarboxylase (EC 4.1.1.33)
• PFAM:
  S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 31.9)
  no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 30.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.67
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004285
  • TAT(Tat/SPI): 0.000159
  • LIPO(Sec/SPII): 0.000933
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162286 NCBI
• RefSeq: YP_493276 NCBI
• UniProt: A0A0H2XIV6 UniProt

⊟Protein sequence

• MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTETKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAAGLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDETSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLAEAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGYPCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: mvk > mvaD > SAUSA300_0574

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications