PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0574 [new locus tag: SAUSA300_RS03070 ]
• pan locus tag^?: SAUPAN002373000
• symbol: SAUSA300_0574
• pan gene symbol^?: mvaK2
• synonym:
• product: phosphomevalonate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0574 [new locus tag: SAUSA300_RS03070 ]
• symbol: SAUSA300_0574
• product: phosphomevalonate kinase
• replicon: chromosome
• strand: +
• coordinates: 648612..649688
• length: 1077
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913148 NCBI
• RefSeq: YP_493277 NCBI
• BioCyc: see SAUSA300_RS03070
• MicrobesOnline: 1292089 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  ATGATTCAGGTCAAAGCACCCGGAAAACTTTATATTGCTGGAGAATATGCTGTAACAGAA
  CCAGGATATAAATCTGTACTTATTGCGTTAGATCGTTTTGTAACTGCTACTATTGAAGAA
  ACAGACCAATATAAAGGTACCATTCATTCAAAAGCATTACATCATAACCCAGTTACATTT
  AGTAGAGATGAAGATAGTATTGTCATTTCAGATCCACATGCAGCAAAACAATTAAATTAT
  GTGGTCACAGCTATTGAAATATTTGAACAATACGCGAAAAGTTGCGATATAGCGATGAAG
  CATTTTCATCTGACTATTGATAGTAATTTAGATGATTCAAATGGTCATAAATATGGATTA
  GGTTCAAGTGCAGCAGTACTTGTGTCAGTTATAAAAGTATTAAATGAATTTTATGATATG
  AAGTTATCTAATTTATACATTTATAAACTAGCAGTGATTGCAAATATGAAGTTACAAAGT
  TTAAGTTCATGCGGAGATATTGCTGTGAGTGTATATAGTGGATGGCTAGCGTATAGTACT
  TTTGATCATGAATGGGTTAAGCATCAAATTGAAGATACTACGGTTGAAGAAGTTTTAATC
  AAAAACTGGCCTGGATTGCACATCGAACCATTACAAGCACCTGAAAATATGGAAGTACTT
  ATCGGTTGGACTGGCTCACCGGCGTCATCACCACACTTTGTTAGCGAAGTGAAACGTTTG
  AAATCAGATCCTTCATTTTACGGTGACTTCTTAGAAGATTCACATCGTTGTGTTGAAAAA
  CTTATTCATGCTTTTAAAACAAATAACATTAAAGGTGTGCAAAAGATGGTGCGTCAGAAT
  CGTACAATTATTCAACGTATGGATAAAGAAGCTACAGTTGATATAGAAACTGAAAAGCTA
  AAATATTTGTGTGATATTGCTGAAAAGTATCACGGCGCATCTAAAACATCAGGCGCTGGT
  GGTGGAGACTGTGGTATTACAATTATCAATAAAGATGTAGATAAAGAAAAAATTTATGAT
  GAATGGACAAAACATGGTATTAAACCATTAAAATTTAATATTTATCATGGGCAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0574 [new locus tag: SAUSA300_RS03070 ]
• symbol: SAUSA300_0574
• description: phosphomevalonate kinase
• length: 358
• theoretical pI: 6.50932
• theoretical MW: 40246.6
• GRAVY: -0.279609

⊟Function

• reaction:
  EC 2.7.4.2?  ExPASy
  Phosphomevalonate kinase ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate
• TIGRFAM:
  Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 584.8)
  and 5 more
  Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 92.3)
  Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01219; EC 2.7.4.2; HMM-score: 59.8)
  Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 22.3)
  Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 13.7)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 12.9)
• TheSEED  :
  • Phosphomevalonate kinase (EC 2.7.4.2)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Phosphomevalonate kinase (EC 2.7.4.2)
  and 1 more
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Phosphomevalonate kinase (EC 2.7.4.2)
• PFAM:
  S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 48.6)
  no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 42.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004935
  • TAT(Tat/SPI): 0.000312
  • LIPO(Sec/SPII): 0.000793
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160430 NCBI
• RefSeq: YP_493277 NCBI
• UniProt: A0A0H2XEZ9 UniProt

⊟Protein sequence

• MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQYKGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHFHLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQSLSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENMEVLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKMVRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVDKEKIYDEWTKHGIKPLKFNIYHGQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: mvk > mvaD > SAUSA300_0574

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications