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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00579
  • pan locus tag?: SAUPAN002373000
  • symbol: SAOUHSC_00579
  • pan gene symbol?: mvaK2
  • synonym:
  • product: phosphomevalonate kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00579
  • symbol: SAOUHSC_00579
  • product: phosphomevalonate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 581406..582482
  • length: 1077
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTCAGGTCAAAGCACCCGGAAAACTTTATATTGCTGGAGAATATGCTGTAACAGAA
    CCAGGATATAAATCTGTACTTATTGCGTTAGATCGTTTTGTAACTGCTACTATTGAAGAA
    GCAGACCAATATAAAGGTACCATTCATTCAAAAGCATTACATCATAACCCAGTTACATTT
    AGTAGAGATGAAGATAGTATTGTCATTTCAGATCCACATGCAGCAAAACAATTAAATTAT
    GTGGTCACAGCTATTGAAATATTTGAACAATACGCGAAAAGTTGCGATATAGCGATGAAG
    CATTTTCATCTGACTATTGATAGTAATTTAGATGATTCAAATGGTCATAAATATGGATTA
    GGTTCAAGTGCAGCAGTACTTGTGTCAGTTATAAAAGTATTAAATGAATTTTATGATATG
    AAGTTATCTAATTTATACATTTATAAACTAGCAGTGATTGCAAATATGAAGTTACAAAGT
    TTAAGTTCATGCGGAGATATTGCTGTGAGTGTATATAGTGGATGGCTAGCGTATAGTACT
    TTTGATCATGAATGGGTTAAGCATCAAATTGAAGATACTACGGTTGAAGAAGTTTTAATC
    AAAAACTGGCCTGGATTGCACATCGAACCATTACAAGCACCTGAAAATATGGAAGTACTT
    ATCGGTTGGACTGGCTCACCGGCGTCATCACCACACTTTGTTAGCGAAGTGAAACGTTTG
    AAATCAGATCCTTCATTTTACGGTGACTTCTTAGAAGATTCACATCGTTGTGTTGAAAAA
    CTTATTCATGCTTTTAAAACAAATAACATTAAAGGTGTGCAAAAGATGGTGCGTCAGAAT
    CGTACAATTATTCAACGTATGGATAAAGAAGCTACAGTTGATATAGAAACTGAAAAGCTA
    AAATATTTGTGTGATATTGCTGAAAAGTATCACGGCGCATCTAAAACATCAGGCGCTGGT
    GGTGGAGACTGTGGTATTACAATTATCAATAAAGATGTAGATAAAGAAAAAATTTATGAT
    GAATGGACAAAACATGGTATTAAACCATTAAAATTTAATATTTATCATGGGCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00579
  • symbol: SAOUHSC_00579
  • description: phosphomevalonate kinase
  • length: 358
  • theoretical pI: 6.50932
  • theoretical MW: 40216.6
  • GRAVY: -0.272626

Function[edit | edit source]

  • reaction:
    EC 2.7.4.2?  ExPASy
    Phosphomevalonate kinase ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate
  • TIGRFAM:
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 584.7)
    and 5 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 92.4)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01219; EC 2.7.4.2; HMM-score: 59.6)
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 22.1)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 13.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 12.8)
  • TheSEED:  
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Phosphomevalonate kinase (EC 2.7.4.2)
    and 2 more
    archaeal lipids bobik  Phosphomevalonate kinase (EC 2.7.4.2)
    Isoprenoid Biosynthesis  Phosphomevalonate kinase (EC 2.7.4.2)
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 48.6)
    no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 42.6)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004989
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.000758
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQYKGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHFHLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQSLSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENMEVLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKMVRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVDKEKIYDEWTKHGIKPLKFNIYHGQ

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [4] [5]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  5. 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]