From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS03150 [old locus tag: SAUSA300_0587 ]
  • pan locus tag?: SAUPAN002444000
  • symbol: SAUSA300_RS03150
  • pan gene symbol?: —
  • synonym:
  • product: DUF443 domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS03150 [old locus tag: SAUSA300_0587 ]
  • symbol: SAUSA300_RS03150
  • product: DUF443 domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 660995..661630
  • length: 636
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGCTATGTGAATCTAAAGTTATCAACAAAAATCCTAAGTATAGAGTTATTAAATATGGT
    GATGAATATTTAATGATTGATTTAGTAAGTACCTGGTTAACTTTATTTCTTCCTATGATT
    AATTGGTTAATTCCAAAAAAATATGTCAAAATCAGTAAAAAAGAATTTGACGATTTAAAC
    ATTGTCAAACCTGTTAAAAATAAAGCTTTTTGGCCAGTTGCAGGTAGTACTATTTTGTTC
    GGAGTTACGTTTAGAAAATATATTCCTTCACTTAATATTCAATTAGAGAAAAACATGGTG
    ATTGTAATATGTTGTGCAATATTTCTGGGTGTTTTAATACTTTTTTTATTTCTGAATCGT
    AAGCTAAGGTTGGAAATTTATAATAATAACTCTAGTAAAGGGAAAATAATTTTATTTCCT
    TCATTAAAAAACTTTTGTTTCACAATATTTTATTATTTTTTATTTGGCGGTCTTTCAATA
    ATGGCTCTAAGTATGTTATTAACTTTAAATCCTCAAAATATAATAGGCTTTATTGGTTGG
    TTGGTAATGACTGCAGGTTTCTTTCTGTTAAACATGTCATCGATTATTGACAAAAAAATT
    TATGTATTATCTAAAACTAACACGGTGGAAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    636

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS03150 [old locus tag: SAUSA300_0587 ]
  • symbol: SAUSA300_RS03150
  • description: DUF443 domain-containing protein
  • length: 211
  • theoretical pI: 10.2473
  • theoretical MW: 24428.5
  • GRAVY: 0.535545

⊟Function[edit | edit source]

  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 174.8)
  • TheSEED: see SAUSA300_0587
  • PFAM:
    no clan defined DUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 223.4)
    and 3 more
    DUF5357; Family of unknown function (DUF5357) (PF17310; HMM-score: 16.2)
    LtrA; Bacterial low temperature requirement A protein (LtrA) (PF06772; HMM-score: 14.1)
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003941
    • TAT(Tat/SPI): 0.00019
    • LIPO(Sec/SPII): 0.000681
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLIPKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIVICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSIMALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS03150&oldid=92992"