PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS03245 [old locus tag: SAUSA300_0604 ]
• pan locus tag^?: SAUPAN002483000
• symbol: SAUSA300_RS03245
• pan gene symbol^?: —
• synonym:
• product: alpha/beta hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS03245 [old locus tag: SAUSA300_0604 ]
• symbol: SAUSA300_RS03245
• product: alpha/beta hydrolase
• replicon: chromosome
• strand: +
• coordinates: 677467..678255
• length: 789
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (677467..678255) NCBI
• BioCyc: SAUSA300_RS03245 BioCyc
• MicrobesOnline: see SAUSA300_0604

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATAAAGTCACAATTAATCCTCAAATCCAATTAACTTATCAAATTGAAGGTAAAGGG
  GATCCTATAATATTACTTCATGGATTGGATGGTAATTTAGCTGGATTTGAAGATTTGCAA
  CATCAACTAGCATCATCATATAAAGTACTTACTTACGATTTAAGAGGTCATGGCAAGTCT
  TCTAAAAGTGAATCATACGATTTAAACGATCACGTTGAGGATTTAAAAATTCTAATGGAG
  AAGTTAAATATTCATGAGGCACATATTCTAGGACATGATTTAGGTGGGGTAGTTGCTAAG
  TTATTTACAGATAAATATGCTTATCGTGTAAAATCATTAACTACCATTGCATCGAAGAAA
  GATGACTTAATACACAGCTTTACTCAATTGTTAATACAATATCAAGATGATATAGCGGGT
  TTTAATAAGTCTGAAGCGTATATTCTTTTATTTTCTAAATTGTTTAGAAATCAAGAGAAG
  ACGATGAAATGGTATCAAAAACAAAGAATATATAGCATTAAGTCTGAGGATGATAGTGCG
  GTGGCAATTCGTTCATTAATTTTGCATAAAGATGAACCTATGTATTTAAAAAAACGTACA
  TGTGTACCTACTTTGTTAATTAATGGGGAACATGATCCTTTGATTAAAGATAAGAATCAT
  TTTAAATTGGAAGCGCATTTTTTAAATGTTACGAAAAAAATCTTCGAACATTCAGGACAT
  GCACCGCATATTGAAGAACCAGAAGCATTTATGAATTATTATTTAAAATTTTTAAAAAGC
  GTATCATAA
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  789
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS03245 [old locus tag: SAUSA300_0604 ]
• symbol: SAUSA300_RS03245
• description: alpha/beta hydrolase
• length: 262
• theoretical pI: 7.9427
• theoretical MW: 30215.5
• GRAVY: -0.41374

⊟Function

• reaction:
  EC 3.4.11.5?  ExPASy
  Prolyl aminopeptidase Release of N-terminal proline from a peptide
• TIGRFAM:
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 78.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 76.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 72.9)
  pyrimidine utilization protein D (TIGR03611; HMM-score: 68.1)
  and 6 more
  proline-specific peptidase (TIGR01250; HMM-score: 59.7)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 47.9)
  prolyl aminopeptidase (TIGR01249; EC 3.4.11.5; HMM-score: 35.3)
  2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 34)
  Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 27.1)
  Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 13.2)
• TheSEED: see SAUSA300_0604
• PFAM:
  AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 100.5)
  and 10 more
  Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 61.2)
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 54.5)
  PGAP1; PGAP1-like protein (PF07819; HMM-score: 20.9)
  FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 20.6)
  Ndr; Ndr family (PF03096; HMM-score: 17.8)
  DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 16.6)
  Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 16.4)
  Abhydrolase_4; TAP-like protein (PF08386; HMM-score: 14.1)
  Thioesterase; Thioesterase domain (PF00975; HMM-score: 12.4)
  Lipase_3; Lipase (class 3) (PF01764; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007481
  • TAT(Tat/SPI): 0.000325
  • LIPO(Sec/SPII): 0.000767
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446968165 NCBI
• RefSeq: WP_001045421 NCBI
• UniProt: see SAUSA300_0604

⊟Protein sequence

• MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLASSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLFTDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEKTMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDKNHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications