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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS03455 [old locus tag: SAUSA300_0644 ]
  • pan locus tag?: SAUPAN002537000
  • symbol: SAUSA300_RS03455
  • pan gene symbol?: graX
  • synonym:
  • product: NAD(P)-dependent oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS03455 [old locus tag: SAUSA300_0644 ]
  • symbol: SAUSA300_RS03455
  • product: NAD(P)-dependent oxidoreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 718079..719002
  • length: 924
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACCTAAAGTTTTATTAGCAGGTGGAACAGGATATATTGGTAAGTATTTAAGTGAA
    GTGATTGAAAATGATGCTGAACTTTTTGCTATATCAAAATATCCAGACAATAAAAAAACA
    GATGATGTTGAAATGACTTGGATTCAGTGTGATATATTTCATTACGAACAGGTTGTTGCA
    GCAATGAATCAAATAGATATTGCTGTATTCTTTATCGACCCAACAAAGAATTCTGCCAAA
    ATAACACAATCATCAGCAAGAGATTTAACATTAATCGCAGCAGATAATTTTGGTCGAGCA
    GCGGCTATCAATCAAGTAAAAAAAGTAATCTACATACCTGGGAGTCGTTATGATAATGAA
    ACAATTGAACGCCTAGGTGCATATGGTACACCTGTAGAAACAACAAATTTAGTTTTTAAA
    CGTTCTTTAGTTAATGTAGAATTACAAGTTTCAAAGTATGATGATGTTAGATCAACGATG
    AAGGTAGTTTTACCAAAGGGATGGACATTAAAGAACGTTGTAAACCATTTTATTGCATGG
    ATGGGTTACACTAAAGGAACTTTTGTGAAAACAGAAAAATCACATGATCAATTTAAGATA
    TATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGA
    ATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGG
    AAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTAT
    ATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATG
    ATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGA
    GAAAATATGAAATATACTAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS03455 [old locus tag: SAUSA300_0644 ]
  • symbol: SAUSA300_RS03455
  • description: NAD(P)-dependent oxidoreductase
  • length: 307
  • theoretical pI: 9.62557
  • theoretical MW: 35234.8
  • GRAVY: -0.14886

⊟Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 13)
  • TheSEED: see SAUSA300_0644
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 23.5)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 22.8)
    and 1 more
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 15.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016804
    • TAT(Tat/SPI): 0.00014
    • LIPO(Sec/SPII): 0.001705
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVEMTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAAINQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTMKVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETADGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPMQKMMIHGFEVDCRIKDFQSRLKSGENMKYTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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