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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS03990 [old locus tag: SAUSA300_0740 ]
  • pan locus tag?: SAUPAN002670000
  • symbol: SAUSA300_RS03990
  • pan gene symbol?:
  • synonym:
  • product: hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS03990 [old locus tag: SAUSA300_0740 ]
  • symbol: SAUSA300_RS03990
  • product: hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 822793..823443
  • length: 651
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGTGTACATCAATATTTTAAAAGATTATCAGATATGGAAAGACTTATAAGATTACCT
    GGAAAATTTAAATATTTTGAACACAATGTTGCAGCACACTCCTTTAAAGTAACTAAAATT
    GCACAATATCTAGCAACAGTTGAAGAATATCATGGACGAAAGATTAATTGGAAAAGCTTA
    TATGAAAAAGCATTAAATCATGATTTCGCCGAAGTGTTTACTGGTGATATAAAAACACCT
    GTTAAATATGCGAGTAGTGAATTAAAAAAATTATTTTCGCAAGTTGAAGAAGAAATGGTA
    GAGACCTTTATTGAAGAAGAAATTCCATTACAATATAGAGATGTTTATAAGCAACGACTG
    CAAGAAGGTAAAGATGATTCATTAGAAGGCCAAATACTTTCAGTTGCTGATAAAATTGAT
    TTGCTTTATGAAACATTTGGAGAAATACAAAAACGTAATCCCGAAGAATTATTTTTCGAA
    ATTTATGAAATGAGTCTAGAAACAATTATTCAATTTGACCATTTAGCATCTGTACAAGAT
    TTTATTAATAATATCATTCCAGAAATGTTGACTGAAAACTTTATACCTAGAACAGAATTA
    AGAGAAACAACCATGAACATTTTAAATAAAAGAAAAGAGGAAAATGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    651

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS03990 [old locus tag: SAUSA300_0740 ]
  • symbol: SAUSA300_RS03990
  • description: hydrolase
  • length: 216
  • theoretical pI: 4.80408
  • theoretical MW: 25704
  • GRAVY: -0.559722

Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00295 family protein (TIGR00295; HMM-score: 16)
  • TheSEED: see SAUSA300_0740
  • PFAM:
    HD_PDEase (CL0237) HD_2; HD containing hydrolase-like enzyme (PF12917; HMM-score: 280)
    and 2 more
    HD_3; HD domain (PF13023; HMM-score: 34.1)
    HD; HD domain (PF01966; HMM-score: 19.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002732
    • TAT(Tat/SPI): 0.00025
    • LIPO(Sec/SPII): 0.000669
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYLATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVETFIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFEIYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]