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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04255 [old locus tag: SAUSA300_0788 ]
  • pan locus tag?: SAUPAN002818000
  • symbol: SAUSA300_RS04255
  • pan gene symbol?: ntrA
  • synonym:
  • product: nitroreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04255 [old locus tag: SAUSA300_0788 ]
  • symbol: SAUSA300_RS04255
  • product: nitroreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 874530..875069
  • length: 540
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGAATTACAACAAGCAATAGCTAATAGAAGAAGTGTGAAAAAATTTAAAAGAGATATG
    CACATAGATGACGCATTGCTATATCAAGCAATTGAGAAAGCTGCTGATGCTCCAAATCAC
    GGAATGAGGGAACCATGGAGAGTTGTGCATGTTCCGAAAGACAGATTAGGAGATATGAGT
    AAGGATATTTCTAAATTTGCATTTCCTAATGAATTAGATAAGCAACAATGTCATTATGAT
    GCAGTTACGAAACTAGGTGGCATGTTATTGCTTATTTTAAAAACAGATCCAAGACAACGT
    CAAAATGATGAAAACTACTTTGCATTTGGTGCATATGCACAAAATCTTATGTTGTTACTT
    TATGAAGCGGGAATAGGTACATGTTGGAAATCGCCATTATATATCTATGATCCTAAAGTA
    AGAAAAACACTTGGTATAAAGAAAGATGAAGTTCTTGCTGGATTCTTATATTTAACGGAT
    TTAGAAGGAGATATGCCTAAAGCACCACGTAAAAATAGAAACTTAATTACATTATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS04255 [old locus tag: SAUSA300_0788 ]
  • symbol: SAUSA300_RS04255
  • description: nitroreductase
  • length: 179
  • theoretical pI: 9.4401
  • theoretical MW: 20676.9
  • GRAVY: -0.496089

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other F420 biosynthesis protein FbiB, C-terminal domain (TIGR03553; HMM-score: 33)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5,6-dimethylbenzimidazole synthase (TIGR02476; EC 1.14.99.40; HMM-score: 32.7)
  • TheSEED: see SAUSA300_0788
  • PFAM:
    FMN-dep-NRtase (CL0529) Nitroreductase; Nitroreductase family (PF00881; HMM-score: 78.7)
    and 1 more
    TM1586_NiRdase; Putative TM nitroreductase (PF14512; HMM-score: 23.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00342
    • TAT(Tat/SPI): 0.000401
    • LIPO(Sec/SPII): 0.000649
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMREPWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQNDENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTDLEGDMPKAPRKNRNLITLY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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