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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS04590 [old locus tag: SAUSA300_0850 ]
- pan locus tag?: SAUPAN003048000
- symbol: SAUSA300_RS04590
- pan gene symbol?: mnhF1
- synonym:
- product: Na+/H+ antiporter subunit F
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS04590 [old locus tag: SAUSA300_0850 ]
- symbol: SAUSA300_RS04590
- product: Na+/H+ antiporter subunit F
- replicon: chromosome
- strand: -
- coordinates: 928664..928957
- length: 294
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (928664..928957) NCBI
- BioCyc: SAUSA300_RS04590 BioCyc
- MicrobesOnline: see SAUSA300_0850
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241ATGAATCATAATGTTATTATCGTTATTGCATTAATCATAGTTGTCATTTCTATGTTAGCT
ATGCTCATTCGCGTTGTGCTAGGCCCATCACTTGCCGATCGTGTTGTCGCATTAGATGCG
ATTGGTCTTCAATTAATGGCAGTTATAGCATTATTCAGTATTTTATTAAATATTAAATAC
ATGATTGTCGTTATTATGATGATTGGTATATTAGCTTTTTTAGGTACTGCAGTATTCTCT
AAATTTATGGACAAAGGTAAGGTGATTGAACATGATCAAAATCATACTGATTAG60
120
180
240
294
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS04590 [old locus tag: SAUSA300_0850 ]
- symbol: SAUSA300_RS04590
- description: Na+/H+ antiporter subunit F
- length: 97
- theoretical pI: 7.7971
- theoretical MW: 10616.1
- GRAVY: 1.3701
⊟Function[edit | edit source]
- reaction: EC 2.3.1.-? ExPASy
- TIGRFAM: Mobile and extrachromosomal element functions Plasmid functions entry exclusion protein TrbK (TIGR04361; HMM-score: 9.9)and 1 moreTIGR03943 family protein (TIGR03943; HMM-score: 6.5)
- TheSEED: see SAUSA300_0850
- PFAM: no clan defined MrpF_PhaF; Multiple resistance and pH regulation protein F (MrpF / PhaF) (PF04066; HMM-score: 49.9)and 2 moreABC-2 (CL0181) ABC2_membrane_5; ABC-2 family transporter protein (PF13346; HMM-score: 14.5)no clan defined Deltameth_res; Deltamethrin resistance (PF16020; HMM-score: 6.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 3
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.033588
- TAT(Tat/SPI): 0.000316
- LIPO(Sec/SPII): 0.021791
- predicted transmembrane helices (TMHMM): 3
⊟Accession numbers[edit | edit source]
- GI: 446939050 NCBI
- RefSeq: WP_001016306 NCBI
- UniProt: see SAUSA300_0850
⊟Protein sequence[edit | edit source]
- MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQLMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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