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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04950 [old locus tag: SAUSA300_0921 ]
  • pan locus tag?: SAUPAN003199000
  • symbol: SAUSA300_RS04950
  • pan gene symbol?: prfC
  • synonym:
  • product: peptide chain release factor 3

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04950 [old locus tag: SAUSA300_0921 ]
  • symbol: SAUSA300_RS04950
  • product: peptide chain release factor 3
  • replicon: chromosome
  • strand: +
  • coordinates: 1010345..1011907
  • length: 1563
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAACTTAAAGCAAGAAGTTGAGTCTAGAAAGACTTTTGCGATTATTTCACATCCCGAT
    GCAGGGAAAACAACGTTAACTGAAAAACTATTGTACTTCAGTGGTGCTATTCGTGAAGCG
    GGTACAGTTAAAGGGAAGAAGACTGGTAAATTTGCGACAAGTGACTGGATGAAAGTTGAA
    CAAGAGCGTGGTATTTCTGTAACTAGTTCAGTAATGCAATTTGATTACGATGATTATAAA
    ATCAATATCTTAGATACACCAGGACATGAAGACTTTTCAGAAGATACGTATAGAACATTA
    ATGGCAGTTGACAGTGCTGTCATGGTCATAGACTGTGCAAAAGGTATTGAACCACAAACA
    TTGAAGTTATTTAAAGTTTGTAAAATGCGTGGTATTCCAATCTTTACATTCATTAATAAA
    TTAGACCGAGTAGGTAAAGAACCATTTGAATTATTAGATGAAATCGAAGAGACATTAAAT
    ATTGAAACATACCCTATGAATTGGCCAATTGGTATGGGACAAAGTTTCTTTGGCATCATT
    GATAGAAAGTCTAAAACAATTGAACCATTTAGAGATGAAGAAAATATATTACATTTGAAT
    GATGATTTTGAGTTGGAAGAAGATCATGCAATTACAAATGATAGTGATTTTGAACAAGCG
    ATTGAAGAATTAATGTTGGTTGAAGAAGCGGGTGAAGCCTTTGATAATGACGCGCTGTTG
    AGTGGAGACTTAACACCTGTATTTTTCGGTTCAGCTTTAGCTAACTTTGGTGTACAAAAT
    TTCTTAAATGCATATGTTGATTTTGCGCCAATGCCAAATGCGAGACAAACAAAAGAAGAC
    GTTGAAGTAAGCCCGTTTGATGATTCATTTTCAGGATTTATCTTTAAAATTCAAGCCAAC
    ATGGACCCTAAACACCGTGATAGAATTGCCTTTATGCGTGTCGTTAGTGGTGCATTTGAA
    CGTGGTATGGATGTTACTTTGCAACGTACTAATAAAAAGCAAAAGATCACACGTTCAACG
    TCATTTATGGCAGACGATAAAGAAACTGTGAATCATGCTGTAGCAGGCGATATCATTGGA
    CTATATGATACTGGTAATTATCAAATTGGAGATACTTTAGTTGGTGGAAAACAAACCTAC
    AGTTTCCAAGATTTACCACAATTTACGCCAGAAATTTTTATGAAAGTTTCTGCTAAAAAC
    GTCATGAAACAGAAGCATTTCCATAAAGGTATTGAACAATTAGTACAAGAAGGTGCGATT
    CAATACTATAAAACATTACACACAAACCAAATTATTTTAGGTGCTGTTGGTCAGTTACAA
    TTTGAAGTTTTCGAACATAGAATGAAAAACGAATATAATGTTGATGTTGTTATGGAGCCA
    GTAGGCCGTAAAATTGCACGTTGGATTGAAAATGAAGACCAAATTACAGATAAGATGAAC
    ACATCAAGATCGATTTTAGTGAAAGATAGATATGACGATTTAGTATTCTTATTTGAAAAT
    GAATTTGCAACAAGATGGTTTGAAGAGAAATTCCCTGAAATTAAATTGTATAGTTTACTT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1563

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS04950 [old locus tag: SAUSA300_0921 ]
  • symbol: SAUSA300_RS04950
  • description: peptide chain release factor 3
  • length: 520
  • theoretical pI: 4.61861
  • theoretical MW: 59601.2
  • GRAVY: -0.390577

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 3 (TIGR00503; HMM-score: 647.7)
    and 18 more
    Genetic information processing Protein synthesis Translation factors translation elongation factor G (TIGR00484; HMM-score: 250.4)
    Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 123.4)
    Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 117.3)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 88.2)
    Genetic information processing Protein synthesis Translation factors translation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 66.5)
    Genetic information processing Protein synthesis Translation factors translation elongation factor Tu (TIGR00485; HMM-score: 61.2)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 55.5)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 48.7)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 45.8)
    Genetic information processing Protein synthesis Translation factors translation initiation factor aIF-2 (TIGR00491; HMM-score: 40.5)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 28.3)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 24.6)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 17.7)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 12.2)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 11.6)
  • TheSEED: see SAUSA300_0921
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 187.3)
    EF-G_C (CL0437) RF3_C; Class II release factor RF3, C-terminal domain (PF16658; HMM-score: 154.7)
    and 5 more
    EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 48.1)
    EF-G_C (CL0437) EFG_II; Elongation Factor G, domain II (PF14492; HMM-score: 36.3)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 30.3)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 17.9)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013097
    • TAT(Tat/SPI): 0.002964
    • LIPO(Sec/SPII): 0.001929
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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