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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS05820 [old locus tag: SAUSA300_1073 ]
  • pan locus tag?: SAUPAN003449000
  • symbol: SAUSA300_RS05820
  • pan gene symbol?: mraW
  • synonym:
  • product: ribosomal RNA small subunit methyltransferase H

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS05820 [old locus tag: SAUSA300_1073 ]
  • symbol: SAUSA300_RS05820
  • product: ribosomal RNA small subunit methyltransferase H
  • replicon: chromosome
  • strand: +
  • coordinates: 1172490..1173425
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAA
    AATGGTGTGTACATTGACTGTACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAAT
    CAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAGACCAAACTGCAATTGATAAT
    GCTAAAGAGGTATTAAAGGATCATTTGCATAAGGTGACTTTTGTTCATAGCAACTTCCGT
    GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGAC
    TTGGGTGTTTCAAGCCCACAACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGAC
    GCAACATTAGACATGCGTATGGACCAAACACAAGAACTAACAGCATATGAAATTGTTAAC
    AATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGCTATGGCGAGGAGAAATTTTCA
    AAACAGATAGCTCGAAGAATCGAAGCACATCGCGAACAACAACCAATAACAACAACATTA
    GAATTAGTTGACATTATAAAAGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACAT
    CCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAAACGATGAATTGTCAGCTTTT
    GAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGATGGCAGGATTTCGGTAATC
    ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGT
    CCAGAGGTACCAAGAGGATTACCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGT
    GTTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAGATGACAATAACAGAGCACGA
    AGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS05820 [old locus tag: SAUSA300_1073 ]
  • symbol: SAUSA300_RS05820
  • description: ribosomal RNA small subunit methyltransferase H
  • length: 311
  • theoretical pI: 5.97721
  • theoretical MW: 35681.3
  • GRAVY: -0.453055

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.199?  ExPASy
    16S rRNA (cytosine1402-N4)-methyltransferase S-adenosyl-L-methionine + cytosine1402 in 16S rRNA = S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(1402)-N(4))-methyltransferase (TIGR00006; EC 2.1.1.199; HMM-score: 409.7)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 15)
  • TheSEED: see SAUSA300_1073
  • PFAM:
    NADP_Rossmann (CL0063) Methyltransf_5; MraW methylase family (PF01795; HMM-score: 456.2)
    and 9 more
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 31.7)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 21.6)
    rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 21.1)
    Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 18.8)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 18.5)
    Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 16.2)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 15.2)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 14.7)
    GCD14; tRNA methyltransferase complex GCD14 subunit (PF08704; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003934
    • TAT(Tat/SPI): 0.000098
    • LIPO(Sec/SPII): 0.000233
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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