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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS06170 [old locus tag: SAUSA300_1140 ]
  • pan locus tag?: SAUPAN003539000
  • symbol: SAUSA300_RS06170
  • pan gene symbol?: lytN
  • synonym:
  • product: cell wall hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS06170 [old locus tag: SAUSA300_1140 ]
  • symbol: SAUSA300_RS06170
  • product: cell wall hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1249155..1250273
  • length: 1119
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATAAACAACAAAGTAAAGTACGCTATTCAATTAGAAAAGTTAGTATTGGAATTTTG
    TCAATTTCAATAGGTATGTTTTTGGCATTGGGTATGTCGAACAAAGCATATGCAGATGAA
    ATTGATAAATCTAAAGATTTTACAAGAGGGTATGAGCAAAATGTATTCGCGAAATCAGAG
    TTAAATGCTAATAAAAATACGACAAAAGACAAAATAAAAAATGAAGGTGCTGTTAAAACA
    TCGGACACAAGTTTAAAGTTAGACAACAAATCAGCAATTTCAAACGGAAATGAAATTAAT
    CAAGATATAAAGATTTCAAATACTCCGAAAAACTCAAGCCAAGGTAACAATCTAGTTATT
    AATAACAATGAACTTACTAAAGAAATTAAAATTGCAAACTTGGAAGCTCAAAATTCTAAT
    CAGAAGAAAACGAATAAAGTTACTAATAATTACTTTGGTTACTACAGTTTTAGAGAAGCT
    CCAAAAACACAAATCTATACTGTAAAAAAAGGAGACACACTTAGTGCTATAGCATTAAAA
    TACAAAACTACAGTTTCAAATATTCAAAATACAAATAATATAGCAAATCCTAATTTAATA
    TTTATTGGTCAAAAATTAAAAGTGCCAATGACACCATTAGTAGAACCAAAACCAAAAACA
    GTGTCTTCAAATAATAAAAGTAATAGTAATAGCAGTACATTAAATTATTTGAAAACATTA
    GAGAATAGAGGATGGGATTTCGACGGTAGTTATGGATGGCAATGTTTCGATTTAGTTAAT
    GTATATTGGAATCATCTTTATGGTCATGGATTAAAAGGATATGGAGCTAAAGATATACCA
    TATGCAAATAATTTTAATAGTGAAGCTAAAATTTATCACAACACACCAACTTTCAAAGCT
    GAACCTGGGGACTTAGTGGTTTTTAGTGGAAGATTTGGTGGAGGATATGGTCATACAGCT
    ATTGTCTTAAATGGTGATTATGATGGAAAATTAATGAAGTTCCAAAGTTTAGATCAAAAC
    TGGAATAATGGTGGATGGCGTAAAGCAGAGGTTGCACATAAAGTTGTTCATAATTATGAA
    AATGATATGATTTTTATTAGACCATTTAAAAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS06170 [old locus tag: SAUSA300_1140 ]
  • symbol: SAUSA300_RS06170
  • description: cell wall hydrolase
  • length: 372
  • theoretical pI: 10.1016
  • theoretical MW: 41754.7
  • GRAVY: -0.704839

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 40.2)
    Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 36.6)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 20.1)
  • TheSEED: see SAUSA300_1140
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 66.1)
    and 2 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 46.4)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 20.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 38 aa
    • SP(Sec/SPI): 0.994665
    • TAT(Tat/SPI): 0.003738
    • LIPO(Sec/SPII): 0.000806
    • Cleavage Site: CS pos: 38-39. AYA-DE. Pr: 0.9957
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKSKDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQDIKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPKTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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