From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1140 [new locus tag: SAUSA300_RS06170 ]
  • pan locus tag?: SAUPAN003539000
  • symbol: lytN
  • pan gene symbol?: lytN
  • synonym:
  • product: cell wall hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1140 [new locus tag: SAUSA300_RS06170 ]
  • symbol: lytN
  • product: cell wall hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1249122..1250273
  • length: 1152
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTTGTATATTATTGTAAGGAGTGTTTCATCATGAATAAACAACAAAGTAAAGTACGC
    TATTCAATTAGAAAAGTTAGTATTGGAATTTTGTCAATTTCAATAGGTATGTTTTTGGCA
    TTGGGTATGTCGAACAAAGCATATGCAGATGAAATTGATAAATCTAAAGATTTTACAAGA
    GGGTATGAGCAAAATGTATTCGCGAAATCAGAGTTAAATGCTAATAAAAATACGACAAAA
    GACAAAATAAAAAATGAAGGTGCTGTTAAAACATCGGACACAAGTTTAAAGTTAGACAAC
    AAATCAGCAATTTCAAACGGAAATGAAATTAATCAAGATATAAAGATTTCAAATACTCCG
    AAAAACTCAAGCCAAGGTAACAATCTAGTTATTAATAACAATGAACTTACTAAAGAAATT
    AAAATTGCAAACTTGGAAGCTCAAAATTCTAATCAGAAGAAAACGAATAAAGTTACTAAT
    AATTACTTTGGTTACTACAGTTTTAGAGAAGCTCCAAAAACACAAATCTATACTGTAAAA
    AAAGGAGACACACTTAGTGCTATAGCATTAAAATACAAAACTACAGTTTCAAATATTCAA
    AATACAAATAATATAGCAAATCCTAATTTAATATTTATTGGTCAAAAATTAAAAGTGCCA
    ATGACACCATTAGTAGAACCAAAACCAAAAACAGTGTCTTCAAATAATAAAAGTAATAGT
    AATAGCAGTACATTAAATTATTTGAAAACATTAGAGAATAGAGGATGGGATTTCGACGGT
    AGTTATGGATGGCAATGTTTCGATTTAGTTAATGTATATTGGAATCATCTTTATGGTCAT
    GGATTAAAAGGATATGGAGCTAAAGATATACCATATGCAAATAATTTTAATAGTGAAGCT
    AAAATTTATCACAACACACCAACTTTCAAAGCTGAACCTGGGGACTTAGTGGTTTTTAGT
    GGAAGATTTGGTGGAGGATATGGTCATACAGCTATTGTCTTAAATGGTGATTATGATGGA
    AAATTAATGAAGTTCCAAAGTTTAGATCAAAACTGGAATAATGGTGGATGGCGTAAAGCA
    GAGGTTGCACATAAAGTTGTTCATAATTATGAAAATGATATGATTTTTATTAGACCATTT
    AAAAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1152

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1140 [new locus tag: SAUSA300_RS06170 ]
  • symbol: LytN
  • description: cell wall hydrolase
  • length: 383
  • theoretical pI: 9.97271
  • theoretical MW: 43182.5
  • GRAVY: -0.655352

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 40.3)
    Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 36.5)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 20)
  • TheSEED  :
    • Putative cell wall hydrolase
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 66)
    and 2 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 46.4)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 20.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: NKAYADEI
  • SignalP: Signal peptide SP(Sec/SPI) length 49 aa
    • SP(Sec/SPI): 0.954742
    • TAT(Tat/SPI): 0.021173
    • LIPO(Sec/SPII): 0.001243
    • Cleavage Site: CS pos: 49-50. AYA-DE. Pr: 0.9741
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFVYYCKECFIMNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKSKDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQDIKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPKTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_1140&oldid=73426"