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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1140 [new locus tag: SAUSA300_RS06170 ]
- pan locus tag?: SAUPAN003539000
- symbol: lytN
- pan gene symbol?: lytN
- synonym:
- product: cell wall hydrolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1140 [new locus tag: SAUSA300_RS06170 ]
- symbol: lytN
- product: cell wall hydrolase
- replicon: chromosome
- strand: +
- coordinates: 1249122..1250273
- length: 1152
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3912995 NCBI
- RefSeq: YP_493837 NCBI
- BioCyc: see SAUSA300_RS06170
- MicrobesOnline: 1292655 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGTTTGTATATTATTGTAAGGAGTGTTTCATCATGAATAAACAACAAAGTAAAGTACGC
TATTCAATTAGAAAAGTTAGTATTGGAATTTTGTCAATTTCAATAGGTATGTTTTTGGCA
TTGGGTATGTCGAACAAAGCATATGCAGATGAAATTGATAAATCTAAAGATTTTACAAGA
GGGTATGAGCAAAATGTATTCGCGAAATCAGAGTTAAATGCTAATAAAAATACGACAAAA
GACAAAATAAAAAATGAAGGTGCTGTTAAAACATCGGACACAAGTTTAAAGTTAGACAAC
AAATCAGCAATTTCAAACGGAAATGAAATTAATCAAGATATAAAGATTTCAAATACTCCG
AAAAACTCAAGCCAAGGTAACAATCTAGTTATTAATAACAATGAACTTACTAAAGAAATT
AAAATTGCAAACTTGGAAGCTCAAAATTCTAATCAGAAGAAAACGAATAAAGTTACTAAT
AATTACTTTGGTTACTACAGTTTTAGAGAAGCTCCAAAAACACAAATCTATACTGTAAAA
AAAGGAGACACACTTAGTGCTATAGCATTAAAATACAAAACTACAGTTTCAAATATTCAA
AATACAAATAATATAGCAAATCCTAATTTAATATTTATTGGTCAAAAATTAAAAGTGCCA
ATGACACCATTAGTAGAACCAAAACCAAAAACAGTGTCTTCAAATAATAAAAGTAATAGT
AATAGCAGTACATTAAATTATTTGAAAACATTAGAGAATAGAGGATGGGATTTCGACGGT
AGTTATGGATGGCAATGTTTCGATTTAGTTAATGTATATTGGAATCATCTTTATGGTCAT
GGATTAAAAGGATATGGAGCTAAAGATATACCATATGCAAATAATTTTAATAGTGAAGCT
AAAATTTATCACAACACACCAACTTTCAAAGCTGAACCTGGGGACTTAGTGGTTTTTAGT
GGAAGATTTGGTGGAGGATATGGTCATACAGCTATTGTCTTAAATGGTGATTATGATGGA
AAATTAATGAAGTTCCAAAGTTTAGATCAAAACTGGAATAATGGTGGATGGCGTAAAGCA
GAGGTTGCACATAAAGTTGTTCATAATTATGAAAATGATATGATTTTTATTAGACCATTT
AAAAAAGCATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1140 [new locus tag: SAUSA300_RS06170 ]
- symbol: LytN
- description: cell wall hydrolase
- length: 383
- theoretical pI: 9.97271
- theoretical MW: 43182.5
- GRAVY: -0.655352
⊟Function[edit | edit source]
- TIGRFAM: gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 40.3)Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 36.5)and 1 moreCellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 20)
- TheSEED :
- Putative cell wall hydrolase
- PFAM: LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 66)and 2 morePeptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 46.4)no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 20.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.32
- Cytoplasmic Membrane Score: 9.55
- Cellwall Score: 0.12
- Extracellular Score: 0.01
- Internal Helix: 1
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: NKAYADEI
- SignalP: Signal peptide SP(Sec/SPI) length 49 aa
- SP(Sec/SPI): 0.954742
- TAT(Tat/SPI): 0.021173
- LIPO(Sec/SPII): 0.001243
- Cleavage Site: CS pos: 49-50. AYA-DE. Pr: 0.9741
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MFVYYCKECFIMNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKSKDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQDIKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPKTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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