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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1141 [new locus tag: SAUSA300_RS06175 ]
- pan locus tag?: SAUPAN003542000
- symbol: SAUSA300_1141
- pan gene symbol?: fmhC
- synonym:
- product: endopeptidase resistance protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1141 [new locus tag: SAUSA300_RS06175 ]
- symbol: SAUSA300_1141
- product: endopeptidase resistance protein
- replicon: chromosome
- strand: +
- coordinates: 1250301..1251545
- length: 1245
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3912983 NCBI
- RefSeq: YP_493838 NCBI
- BioCyc: see SAUSA300_RS06175
- MicrobesOnline: 1292656 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGAAATTTTCAACTTTAAGTGAAGAAGAATTTACCAACTACACCAAAAAGCACTTCAAA
CATTATACACAGTCTATAGAATTATATAATTATAGAAATAAAATAAATCATGAAGCACAT
ATTGTGGGAGTGAAGAATGATAAAAATGAAGTTATAGCTGCATGTTTATTAACAGAGGCA
CGAATTTTTAAATTCTACAAATATTTCTACTCTCATAGAGGTCCTTTACTTGATTATTTC
GATGCTAAATTAGTTTGTTACTTTTTTAAAGAATTATCTAAATTCATTTATAAAAATAGA
GGAGTATTTATTCTTGTTGATCCATATTTAATAGAGAATTTAAGAGATGCAAATGGTAGG
ATAATAAAGAATTATAATAATTCAGTGATAGTAAAGATGCTAGGGAAAATTGGGTATCTC
CATCAAGGTTATACAACAGGATATTCAAATAAAAGTCAAATTAGGTGGATTTCTGTATTG
GATTTAAAAGATAAAGATGAGAATCAACTTTTAAAAGAAATGGAATACCAAACTAGAAGA
AATATAAAAAAGACTATTGAGATTGGTGTTAAGGTTGAAGATTTATCTATTGAAGAAACA
AATCGATTTTATAAATTGTTTCAAATGGCTGAAGAAAAACATGGTTTTCATTTCATGAAT
GAAGATTATTTTAAACGAATGCAAGAAATATATAAAGATAAGGCAATGTTAAAGATAGCT
TGTATAAATCTTAATGAATATCAAGATAAATTAAAAATACAATTATTGAAAATCGAAAAT
GAAATGATGACTGTGAACAGAGCATTAAATGAAAATCCAAATTCTAAAAAAAATAAATCA
AAATTAAATCAGTTAAATATGCAATTATCTAGTATTAATAATAGAATTAGTAAAACCGAA
GAACTAATATTTGAAGATGGACCTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACT
GATGATGAAGTTTATTATCTATCAAGTGGATCAAATCCGAAATATAATCAGTATATGGGT
GCATATCATCTACAATGGCATATGATAAAATATGCAAAATCACATAATATTAATAGGTAT
AATTTTTATGGAATAACAGGCGTCTTTAGTAATGAGGCGGATGATTTTGGTGTTCAACAA
TTTAAAAAGGGTTTTAATGCACATGTTGAAGAATTAATTGGTGATTTCATCAAACCAGTA
AGACCAATTCTATATAAATTTGCAAAACTTATTTATAAGGTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1141 [new locus tag: SAUSA300_RS06175 ]
- symbol: SAUSA300_1141
- description: endopeptidase resistance protein
- length: 414
- theoretical pI: 9.38877
- theoretical MW: 49116.2
- GRAVY: -0.519807
⊟Function[edit | edit source]
- TIGRFAM: Unknown function General FemAB-related protein, PEP-CTERM system-associated (TIGR03019; HMM-score: 22.4)
- TheSEED :
- FmhC protein of FemAB family
- PFAM: Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 487.7)and 3 moreAcetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 24.6)P-loop_NTPase (CL0023) DUF3584; Protein of unknown function (DUF3584) (PF12128; HMM-score: 17.5)no clan defined LRRFIP; LRRFIP family (PF09738; HMM-score: 14.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.013322
- TAT(Tat/SPI): 0.000284
- LIPO(Sec/SPII): 0.002296
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGVKNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGVFILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
FmhC binds to FemB to alter assembly of the pentaglycine cross-bridge linking staphylococcal peptidoglycan. This change converts the fifth glycine of the pentaglycine cross-bridge into a serine providing resistance to lysostaphin and similar staphylococcal cell wall hydrolases. Incorporation of serine at position 5, however, does decrease resistance to beta lactam antibiotics.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
- ↑ Stephanie Willing, Emma Dyer, Olaf Schneewind, Dominique Missiakas
FmhA and FmhC of Staphylococcus aureus incorporate serine residues into peptidoglycan cross-bridges.
J Biol Chem: 2020, 295(39);13664-13676
[PubMed:32759309] [WorldCat.org] [DOI] (I p)