From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS06240 [old locus tag: SAUSA300_1152 ]
  • pan locus tag?: SAUPAN003559000
  • symbol: SAUSA300_RS06240
  • pan gene symbol?: frr
  • synonym:
  • product: ribosome-recycling factor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS06240 [old locus tag: SAUSA300_1152 ]
  • symbol: SAUSA300_RS06240
  • product: ribosome-recycling factor
  • replicon: chromosome
  • strand: +
  • coordinates: 1263474..1264028
  • length: 555
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGTGACATTATTAATGAAACTAAATCAAGAATGCAAAAATCAATCGAAAGCTTATCA
    CGTGAATTAGCTAACATCAGTGCAGGAAGAGCTAATTCAAATTTATTAAACGGCGTAACA
    GTTGATTACTATGGTGCACCAACACCTGTACAACAATTAGCAAGCATCAATGTTCCAGAA
    GCACGTTTACTTGTTATTTCTCCATACGACAAAACTTCTGTAGCTGACATCGAAAAAGCG
    ATAATAGCAGCTAACTTAGGTGTTAACCCAACAAGTGATGGTGAAGTGATACGTATTGCT
    GTACCTGCCTTAACAGAAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGT
    GAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAA
    GATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAG
    AAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGAT
    ATTATGTCAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    555

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS06240 [old locus tag: SAUSA300_1152 ]
  • symbol: SAUSA300_RS06240
  • description: ribosome-recycling factor
  • length: 184
  • theoretical pI: 4.7658
  • theoretical MW: 20352.8
  • GRAVY: -0.584783

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors ribosome recycling factor (TIGR00496; HMM-score: 241.4)
    and 2 more
    Metabolism Energy metabolism ATP-proton motive force interconversion V-type ATPase, G subunit (TIGR01147; EC 3.6.3.14; HMM-score: 16.8)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage tail tape measure protein, lambda family (TIGR01541; HMM-score: 11.9)
  • TheSEED: see SAUSA300_1152
  • PFAM:
    no clan defined RRF; Ribosome recycling factor (PF01765; HMM-score: 206.7)
    and 8 more
    ATP_synthase (CL0255) V-ATPase_G; Vacuolar (H+)-ATPase G subunit (PF03179; HMM-score: 16.6)
    no clan defined RRF_GI; Ribosome recycling factor (PF12614; HMM-score: 16.4)
    Fib_alpha; Fibrinogen alpha/beta chain family (PF08702; HMM-score: 13.4)
    Ribosomal_S6; Ribosomal protein S6 (PF01250; HMM-score: 12.4)
    DUF5082; Domain of unknown function (DUF5082) (PF16888; HMM-score: 10.2)
    YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 9.6)
    Transthyretin (CL0287) DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 9.2)
    S5 (CL0329) Topo-VIb_trans; Topoisomerase VI B subunit, transducer (PF09239; HMM-score: 8.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.041488
    • TAT(Tat/SPI): 0.019998
    • LIPO(Sec/SPII): 0.001671
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYYGAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVPALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQKATDNSIKEIDQMIADKEKDIMSV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS06240&oldid=62679"