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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS06660 [old locus tag: SAUSA300_1229 ]
- pan locus tag?: SAUPAN003703000
- symbol: SAUSA300_RS06660
- pan gene symbol?: —
- synonym:
- product: HAD family phosphatase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS06660 [old locus tag: SAUSA300_1229 ]
- symbol: SAUSA300_RS06660
- product: HAD family phosphatase
- replicon: chromosome
- strand: +
- coordinates: 1346711..1347514
- length: 804
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1346711..1347514) NCBI
- BioCyc: SAUSA300_RS06660 BioCyc
- MicrobesOnline: see SAUSA300_1229
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGACAAATTATAAAGTTGTCGTTTTAGATATGGATGACACATTGCTAAATTCAGATAAT
GTGATATCAGAAGAAACTGCAAATTATTTAACAGCAATTCAAGATGAAGGTTATTATGTT
GTTCTAGCATCTGGTAGACCTACTGAAGGTATGATTCCAACTGCTAGAGATTTAAAATTA
CCTGAACATCATAGCTATATTATTAGTTATAACGGTAGTAAAACGATTAACATGACTAAT
GAAGAAGTAGAAGTAAGTAAATCGATTGGTAAGCAAGATTTCGATGAAATTGTAGATTAT
TGTCGAGATAGAGGCTTTTTCGTTCTTACATATCATGATGGTCAAATTATTTACGACAGC
GAACATGAGTATATGAATATTGAAGCAGAATTAACAGGTTTACCGATGAAACGTGTTGAT
GATATCAAAGCGTATATTCAAGGCGATGTACCCAAGGTCATGGGTGTAGATTATGTAGCG
AATATTACAGAAGCTAGAATTGATTTGAATGGTGTGTTCAATGATAATGTAGATGCTACG
ACAAGTAAGCCATTCTTCTTAGAATTTATGGCCAAAGACGTTTCAAAAGGTAATGCAATT
AAAGCGTTATGTCACAAATTGGGATATTCGGTGGATCAAGTCATTGCTTTTGGTGATAGT
ATGAATGATAAATCAATGTTTGAAGTCGCAGGTCTAGCTATTGCTATGGGGAATGCATCA
GATGAACTTAAGCAATATGCAAATGAAGTTACGTTGGATCATAATGAAAATGGTATTCCA
CATGCGCTCAAAAAATTGTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS06660 [old locus tag: SAUSA300_1229 ]
- symbol: SAUSA300_RS06660
- description: HAD family phosphatase
- length: 267
- theoretical pI: 4.32652
- theoretical MW: 29821.4
- GRAVY: -0.271536
⊟Function[edit | edit source]
- reaction: EC 3.8.1.-? ExPASy
- TIGRFAM: Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 186.2)and 23 morephosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 86.9)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 84.9)sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 81.5)mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 50.4)sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 34.9)Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 33.1)phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 33)sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 32.8)mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 32.3)Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 30.8)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 26.2)Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 23.9)Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 22.9)Energy metabolism Anaerobic phenylphosphate carboxylase, delta subunit (TIGR02726; HMM-score: 22.6)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 22.3)hemimethylated DNA binding domain (TIGR02097; HMM-score: 16.6)HAD phosphatase, family IIIC (TIGR01681; HMM-score: 16)Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 15.4)heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 14.7)HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 14.6)Cellular processes Adaptations to atypical conditions trehalose-phosphatase (TIGR00685; EC 3.1.3.12; HMM-score: 12.8)Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 11.5)Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 11.5)
- TheSEED: see SAUSA300_1229
- PFAM: HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 219.5)and 9 moreS6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 57)Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 27.8)HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 24.4)Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 19.8)DUF2608; Protein of unknown function (DUF2608) (PF11019; HMM-score: 18.3)HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 17.2)no clan defined YccV-like; Hemimethylated DNA-binding protein YccV like (PF08755; HMM-score: 15.9)HAD (CL0137) Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 14.5)no clan defined TMEM240; TMEM240 family (PF15207; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005564
- TAT(Tat/SPI): 0.000279
- LIPO(Sec/SPII): 0.000792
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446108458 NCBI
- RefSeq: WP_000186313 NCBI
- UniProt: see SAUSA300_1229
⊟Protein sequence[edit | edit source]
- MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLASGRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCRDRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVANITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFGDSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY see SAUSA300_1229
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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