From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07410 [old locus tag: SAUSA300_1359 ]
  • pan locus tag?: SAUPAN003930000
  • symbol: SAUSA300_RS07410
  • pan gene symbol?: hepT
  • synonym:
  • product: heptaprenyl diphosphate synthase subunit II

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07410 [old locus tag: SAUSA300_1359 ]
  • symbol: SAUSA300_RS07410
  • product: heptaprenyl diphosphate synthase subunit II
  • replicon: chromosome
  • strand: -
  • coordinates: 1526290..1527234
  • length: 945
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_007793 (1526290..1527234) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACAATGAAATTAAGAAAGTGGAACAACGACTTGAAAAGGCAATAAAAAGTAAAGAT
    TCTGTATTAGAACAGGCATCATTACACTTATTGTCTTCTGGTGGTAAAAGAGTACGCCCA
    GCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGATGAACAAACGTCTGAACAAACGTAT
    CAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCAACACTTGTTCATGATGACGTTATT
    GATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATATCAAAGAAATGGGATCAGACAACT
    GCTATTTTAACTGGGAATTTTTTATTGGCATTAGGACTTGAACACTTAATGGCCGTTAAA
    GATAATCGTGTACATCAATTGATATCTGAATCTATCGTTGATGTTTGTAGAGGGGAACTT
    TTCCAATTTCAAGACCAATTTAACAGTCAACAGACAATTATTAATTATTTACGACGTATC
    AATCGCAAAACAGCACTGTTAATTCAAATATCAACTGAAGTTGGTGCAATTACTTCTCAA
    TCTGATAAAGAGACTGTACGAAAATTGAAAATGATTGGTCATTATATAGGTATGAGCTTC
    CAAATCATTGATGATGTATTAGACTTCACAAGTACCGAAAAGAAATTAGGTAAGCCGGTC
    GGAAGTGATTTGCTTAATGGTCATATTACGTTACCGATTTTATTAGAAATGCGTAAAAAT
    CCAGACTTCAAATTGAAAATCGAACAGTTACGTCGTGATAGTGAACGCAAAGAATTTGAA
    GAATGTATCCAAATCATTAGAAAATCTGACAGCATCGATGAGGCTAAGGCAGTAAGTTCG
    AAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAGTTACCAGATGGACATCCGAAATCA
    CTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGAAACACGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

Protein[edit | edit source]

Protein Data Bank: 5H9D

General[edit | edit source]

  • locus tag: SAUSA300_RS07410 [old locus tag: SAUSA300_1359 ]
  • symbol: SAUSA300_RS07410
  • description: heptaprenyl diphosphate synthase subunit II
  • length: 314
  • theoretical pI: 9.79656
  • theoretical MW: 35584.9
  • GRAVY: -0.357325

Function[edit | edit source]

  • reaction:
    EC 2.5.1.30?  ExPASy
    Heptaprenyl diphosphate synthase (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 377.5)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.2)
  • TheSEED:
  • PFAM:
    Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 222.8)
    and 3 more
    HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 29.4)
    no clan defined Baculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007133
    • TAT(Tat/SPI): 0.001885
    • LIPO(Sec/SPII): 0.001011
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001817755 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]