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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS07950 [old locus tag: SAUSA300_1456 ]
- pan locus tag?: SAUPAN004042000
- symbol: SAUSA300_RS07950
- pan gene symbol?: malA
- synonym:
- product: alpha-glucosidase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS07950 [old locus tag: SAUSA300_1456 ]
- symbol: SAUSA300_RS07950
- product: alpha-glucosidase
- replicon: chromosome
- strand: -
- coordinates: 1609651..1611300
- length: 1650
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1609651..1611300) NCBI
- BioCyc: SAUSA300_RS07950 BioCyc
- MicrobesOnline: see SAUSA300_1456
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1621ATGAATAAGCAATGGTGGAAAGAAGCAGTAGCATATCAAGTATATCCAAGAAGTTTTAAT
GATAGTAATCACGATGGTATTGGGGATTTACCTGGAATGATTGATAAATTGGACTACTTA
AAAGATTTCGGTATCGATGTCATTTGGCTCAGTCCAATGTTTAAATCACCTAATGATGAC
AATGGTTATGATATTAGTGACTACCAAGAGATTATGGATGAATTTGGAACGATGGAAGAC
TTTGATCGTTTATTAAAAGGTGTTCATGATAGAGGCATGAAGCTTATTTTAGATTTAGTT
GTAAATCATACATCTGATGAACATCCTTGGTTTATAGAATCCAAATCTAGTAAAGACAAT
CCCAAACGTGATTGGTACATTTGGCAAGATCCAAAGCCAGATGGCTCTGAACCTAACAAC
TGGGAAAGTATATTTAATGGATCTACATGGGAATATGATGCTAATACTGAGCAATATTAT
TTCCATTTATTCAGTAAAAAACAACCTGATTTGAATTGGGGTAATCCGGAAGTTAGAGAT
GCTGTATTTGAAATGATGAACTGGTGGTTTGATAAAGGCATTGATGGATTTAGAGTAGAT
GCAATTACGCATATTAAGAAGACGTTTGAAGCGGGTGACTTACCTGTACCTGAGGGTAAA
ACATATGCCCCAGCATTTGATGTAGATATGAATCAGCCAGGTATACAAACTTGGTTACAA
GAGATGAAAGATCGCTCATTAAGTAAGTATGACATTATGACTGTTGGTGAAGCGAATGGT
GTAAGCCCTGATGATGCTGATGACTGGGTCGGGGAAGAAAATGGTAAATTTAATATGATA
TTCCAATTTGAACATTTGGGACTGTGGAATAGTGGTGATTCTCACTTTGATGTAAATTCG
TATAAATCTGTATTAAATAGATGGCAAAAACAACTTGAAAATAAAGGTTGGAATGCGTTG
TTTATTGAAAATCACGACCAACCACGACGTGTATCGACGTGGGGTGACGATGACAAGTAT
TGGTATGAATCAGCAACAAGTCATGCAGCTGTTTATTTCTTGCAACAAGGTACGCCATTC
ATTTATCAAGGTCAAGAAATTGGTATGACGAATTATCCATTTGAAAGTATTGAAACGTTT
AACGATGTTGCTGTTAAAAATGACTATCAAATAGTGAAAGCTCAAGGTGGAGATGTAGAC
GCTTTACTTGCGAAATATAAAGATGAGAACCGAGATAATTCTCGCACACCAATGCAATGG
GATGATACGTTAAATGGAGGATTTACAAATGGTGAACCGTGGTTCCCAGTGAATCCGAAT
TATAAAACTATCAATGTTGCACAACAATTAGAAGATGAGCATTCAGTATTACAATTTTAT
AAAGATTTAATTCAATTAAGAAAGTCTAATGATGTATACGTATATGGTCAATTTGATTTA
GTAGATGCTGAAAATTCACAAGTTTTTGCGTACACGAGAACATTAAATGAAAAGCAAGTT
CTTATTGTAGGTAATCTTACTAACCACGAAGCAGAATTAACTGTACCATTTGATTTAAGC
CATGGAGAAGTAAAGCTATTTAATTATGATGCCAAAGTTAATTTAAAACAGTTACGTCCA
TATGAAGCATGTGTTATCGAACTAAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS07950 [old locus tag: SAUSA300_1456 ]
- symbol: SAUSA300_RS07950
- description: alpha-glucosidase
- length: 549
- theoretical pI: 4.30515
- theoretical MW: 63905.2
- GRAVY: -0.736066
⊟Function[edit | edit source]
- reaction: EC 3.2.1.1? ExPASyAlpha-amylase Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose unitsEC 3.2.1.20? ExPASyAlpha-glucosidase Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
- TIGRFAM: alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 597.7)and 9 moreEnergy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 346.7)Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 66.1)Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 62.9)Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 29.2)Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 25.7)pullulanase, extracellular (TIGR02102; HMM-score: 24.4)pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 22.2)Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme (TIGR01531; HMM-score: 12.8)Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 12.4)
- TheSEED: see SAUSA300_1456
- PFAM: Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 380.6)and 4 moreGHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 34.7)Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 17.8)GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.5)DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007678
- TAT(Tat/SPI): 0.000178
- LIPO(Sec/SPII): 0.000843
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446964655 NCBI
- RefSeq: WP_001041911 NCBI
- UniProt: see SAUSA300_1456
⊟Protein sequence[edit | edit source]
- MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFGIDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVNHTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYYFHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPEGKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGKFNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTWGDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIVKAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQLEDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTNHEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA, MalR see SAUSA300_1456
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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